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Organize Your Data from the Big Blue Mutation Assay

dicates the active section. Just click on the tabs and flip through the recipe cards from left to right as information is entered. Location in the data hierarchy is constantly displayed in the guide boxes at the bottom of the screen. These guide boxes are automatically updated as new information is entered. In addition to monitoring hierarchical position, they can also be used to quickly jump to other areas in the data structure by making a selection from the dropdown list in each box. In this way, the accuracy of the data entered lower in the hierarchy can be checked.

The MutaTrak electronic notebook can be used with both the established lacI plaque color-screening method2 and the l Select-cII mutation assay kit3. Once the screening method is chosen, the corresponding fields specific to the particular assay are presented. On the Screening page, mutant information is entered. After DNA sequence information is available, the specific mutation(s) identified for each mutant can be cataloged and categorized in detail (Figure 2).

Figure 2

Reporting

When data entry is complete, a range of reporting tools is available: Using the Mutant Frequency Report, data are collated from separate plating experiments, and mutant frequencies are calculated. The report compares mutant frequencies from two treatment groups. Mutational events are assessed using the Mutational Spectrum Report, which tabulates mutational spectra based on the occurrence of each mutation type. Specialized computations are also performed, such as calculating the percentage of mutations at CpG sites and correcting for possible clonal effects. In addition to the standard re
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