Navigation Links
Oragene and DNA sequencing with ABI PRISM

J. Chartier
DNA Genotek Inc., Ottawa, ON, Canada

DNA from Oragene is reliably sequenced using the ABI PRISM 377 DNA Sequencer.


Introduction
Blood leukocytes are the traditional source of DNA for molecular testing, but collection is invasive and specimens require infectious precautions. Buccal swabs are a less-invasive method of collecting DNA for sequencing applications such as HLA typing and prelingual deafness screening (ref. 1, 2) but they tend to have an appreciable failure rate (ref. 3). Oragene is a non-invasive DNA self-collection kit from saliva that provides significantly higher DNA yields than buccal swabs (ref. 4). The purpose of this study was to investigate the suitability of DNA from Oragene for DNA sequencing.


Materials and Methods
DNA collection
Saliva samples were collected from 5 donors. Collection and purification of DNA was carried out according to protocols supplied with the Oragene kit.

Primer design
PCR primers for the human Thymidylate Synthase (TYMS) gene were designed based on the publicly available DNA sequence (GenBank accession no. AP001178). The primers generate a 560-bp fragment. Table 1 shows the primer sequences.

DNA Sequencing
Oragene-purified DNA from each of the 5 samples was used as the template for PCR with the TS 560 primers. The PCR products were sequenced in both directions by Cortec DNA Service Laboratories (Kingston, ON) using an ABI PRISM 377 DNA Sequencer (Applied Biosystems) and the DYEnamic ET Dye Terminator Kit (Amersham Biosciences). Prior to sequencing, the PCR products
'"/>

Source:


Page: All 1 2

Related biology technology :

1. DNA Stability with Oragene
2. Oragene and Whole Genome Amplification with GenomiPhi
3. DNA Yield with Oragene
4. Oragene is compatible with TaqMan SNP genotyping
5. Serial Analysis of Gene Expression Using ABI PRISM DNA Sequencers and BigDye Terminators
6. Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
7. Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Oragene and DNA sequencing with ABI PRISM

(Date:10/22/2014)... (PRWEB) October 22, 2014 WriteResult, ... services – has been working with a team of ... New Haven Farms Community Farming Collaborative to provide electronic ... solution. The study, which started in May, aims ... that provides participants weekly exposure to and participation in ...
(Date:10/22/2014)... 2014 Physicians Choice Laboratory Services ... menu, which enables healthcare providers to individualize therapeutic ... evidence-based results, healthcare providers are better able to ... while minimizing risks for adverse drug reactions and ... the FDA [1] more than 2.2 million adverse ...
(Date:10/22/2014)... - iCo Therapeutics ("iCo" or "the Company") (TSX-V: ICO) ... Oral Amphotericin B program.  The company recently announced ... work involving samples from HIV/AIDS patients exposed to ... clinical studies and regulatory filings to move forward ... approximately $700,000 of funding and technological advice from ...
(Date:10/22/2014)... DIEGO and HONG KONG ... innovative rare disease therapeutics enterprise, announced today that rare ... the company as vice president, research. Dr. McKew brings ... including key leadership positions at the National Institutes of ... its acquisition by Wyeth). Dr. McKew will lead aTyr,s efforts ...
Breaking Biology Technology:myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 2myPROpad™ Topic of Panel Discussion at the Yale Food Systems Symposium 3PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 2PCLS Announces Expansion of Cardiovascular Pharmacogenetic Testing Menu 3iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 2iCo Therapeutics Announces Advancement of Oral Amphotericin B Program 3aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3
... - Gov. Jim Doyle, during visits to Green Bay and ... production of renewable energy. , ,In what was billed as ... effort to generate 25 percent of electricity and transportation fuel ... energy crisis," Doyle said at Virent Energy Systems ...
... - GE Healthcare , the $15 billion unit ... Translational Genomics Research Institute (TGen), a not-for-profit ... equipment for drug discovery and development. , ,The alliance ... accelerate research in translational genomics, a field that applies ...
... gas pump to the air-conditioning bill, middle class families in ... by the skyrocketing cost of energy. It's simply outrageous that ... car, and Washington continues to allow the oil companies to ... , ,America is facing an energy crisis. It's a crisis ...
Cached Biology Technology:Doyle launches 2025 alternative energy plan 2GE Healthcare partners with genomics institute 2Wisconsin can lead the way to energy independence 2Wisconsin can lead the way to energy independence 3
(Date:10/16/2014)... Respiratory Syncytial Virus causes severe respiratory tract infections ... each year. Scientists at VIB and Ghent University ... to counteract this common virus infection. , ... strategy that paves the way for the development ... a virus that causes suffering in numerous small ...
(Date:10/15/2014)... is a non-traditional biophysics textbook and it describes how ... journey of discovery into biological systems and provides an ... It is about how our genes make proteins that ... of cells in an organism. It quantifies the principles ... can be found on both large and small scales. ...
(Date:10/15/2014)... the 44th Rosenstiel Award for Distinguished Work in Biomedical ... the mechanisms of genomic instability and its implications for ... second alumnus to win the Rosenstiel Award; the first, ... Alt is the Charles A. Janeway Professor of Pediatrics ... an investigator at the Howard Hughes Medical Institute at ...
Breaking Biology News(10 mins):New perspectives for development of an RSV vaccine 2New book about life as seen from physics 2Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2
... VIEW and LA JOLLA, Calif. Oct. 3, 2011 ... genome sequencing company, and the not-for-profit Scripps Health system ... the Wellderly Study, a landmark scientific research initiative aimed ... Under the collaboration, Complete Genomics will sequence, at its ...
... Playing street football two or three times a week could ... led by the Universities of Exeter and Copenhagen, out today ... the fitness of homeless people, a group with typically poor ... much lower-than-average life expectancy, usually as a result of cardiovascular ...
... type of cancer among women in the United States, ... is key in the treatment of breast cancer and ... arrived at University Hospitals (UH) Case Medical Center ... of the breast. "Tomosynthesis offers women the ...
Cached Biology News:Scripps and Complete Genomics to collaborate on genetic research study on healthy aging 2Scripps and Complete Genomics to collaborate on genetic research study on healthy aging 3Could soccer give homeless men a health kick? 2New technology offers the next generation of mammography 2
... (100+kb), fosmid (40kb), and large plasmid insert ... othe BAC vector. On command 20-50 fold ... recombinant DNA purification. Available with blue-white screening ... for the highest insert stability. Kits include ...
Mouse Exodus 2 - 12kDa...
TGF-beta 2 MAb (Clone 8607)...
Abin-2 (N-16)...
Biology Products: