Navigation Links
Optimum FFPE RNA Isolation Kit

respectively.


How can you increase your chances of success with FFPE?
Although the Optimum FFPE Isolation Kit is a robust protocol, there are other uncontrollable factors that can impact the overall quality and yield of RNA isolated from FFPE tissues. Below are a few recommendations to address several key factors.

1) Upstream Tissue Procurement and Tissue Specimen Preparation If possible, tissues should be fixed within one hour of surgical resection. Extensive degradation of RNA can occur before completion of the fixation process. The optimal fixation time is 12-24 hours, using neutral-buffered formalin or paraformaldehyde. Fixed tissues should be thoroughly dehydrated prior to embedding.

2) Tissue Type, Size, and Amount Being Used for RNA Isolation The recommended tissue thickness is 10-20 m (Table 1). The number of sections used is determined by the tissue type (which impacts cell density) and surface area (recommended size: 50-300 mm2). Excess starting material can cause filter clogging, resulting in poor yield.

3) Excessive Amount of Paraffin Used for Embedding Tissues Two times xylene treatment at room temperature should be sufficient for complete deparaffinization. If desired, a more rigorous 37-55C treatment can be done for up to 30 minutes. After the xylene deparaffinization, it is crucial that the 100% ethanol is completely removed and the pellets are d
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Optimum FFPE RNA Isolation Kit

(Date:9/23/2014)... France (PRWEB) September 23, 2014 ... of laboratory personnel since 1968 and Burdinola, supplier of ... of a new filtered fume hood. , ... laboratory fume hood, Burdinola will now offer an innovative ... a wide range of chemicals, best-in-class detection technology, and ...
(Date:9/23/2014)... , Sept. 23, 2014 Research ... "Proteomics Markets for Research and IVD Applications ... report to their offering. ... proteomics applications, both in their research use and ... including the study of the structure and function ...
(Date:9/23/2014)... 2014 For the fifth time since 2006, ... of the fastest-growing companies. , “We’re so proud that ... by Inc. Magazine,” ExakTime CEO Tony Pappas said. , ExakTime ... a three-year growth rate of 61 percent from 2010 to ... more than 40 percent. , Pappas cited strong performance in ...
(Date:9/23/2014)... Jose, California (PRWEB) September 23, 2014 ... Agricultural biotechnology is gaining steady popularity the world ... plant breeding techniques. Growth in the marketplace is ... Modified (GM) crops in developing countries. Several sub-Saharan ... of under-nourished population, have already implemented genetically engineered ...
Breaking Biology Technology:Erlab Partners with Burdinola to Deliver an Innovative Filtered Fume Hood 2Proteomics Markets for Research and IVD Applications (Mass Spectrometry, Chromatography, Microarrays, Electrophoresis, Immunoassays, Other Technologies) 2Proteomics Markets for Research and IVD Applications (Mass Spectrometry, Chromatography, Microarrays, Electrophoresis, Immunoassays, Other Technologies) 3Proteomics Markets for Research and IVD Applications (Mass Spectrometry, Chromatography, Microarrays, Electrophoresis, Immunoassays, Other Technologies) 4ExakTime Makes Inc. 5000 List for the Fifth Time 2Rising Focus on Food Security Spurs Growth in the Global Agricultural Biotechnology Market, According to New Report by Global Industry Analysts, Inc. 2Rising Focus on Food Security Spurs Growth in the Global Agricultural Biotechnology Market, According to New Report by Global Industry Analysts, Inc. 3Rising Focus on Food Security Spurs Growth in the Global Agricultural Biotechnology Market, According to New Report by Global Industry Analysts, Inc. 4
... a material in which an electric field can control the ... effect discovered by the Rutgers group can be extended to ... bits in ultra high-density data storage. The research appears in ... The researchers found the effect by studying the magnetic properties ...
... ROCKVILLE, Md., Aug. 22 A research team co-led by ... Institute for Genome Sciences (IGS), University of Maryland School of ... bean ( Ricinus communis ) genome in Nature Biotechnology ... IGS were co-lead authors on the paper describing the 4.5X ...
... Trial Confirms Clinical Efficacy and Safety of Radioembolisation Using... -- BRUSSELS, August 19, 2010 ... ... --> ... s = ,; var first_result; // Results are keyed by longUrl, so we need to grab ...
Cached Biology Technology:Castor Bean Genome Published by Research Team Including Scientists from the Venter Institute 2Castor Bean Genome Published by Research Team Including Scientists from the Venter Institute 3Phase III Randomised Controlled Trial Confirms Clinical Efficacy and Safety of Radioembolisation Using 90Y-resin Microspheres for Patients With Inoperable Colorectal Cancer Liver Metastases That Have Failed Chemotherapy 2Phase III Randomised Controlled Trial Confirms Clinical Efficacy and Safety of Radioembolisation Using 90Y-resin Microspheres for Patients With Inoperable Colorectal Cancer Liver Metastases That Have Failed Chemotherapy 3Phase III Randomised Controlled Trial Confirms Clinical Efficacy and Safety of Radioembolisation Using 90Y-resin Microspheres for Patients With Inoperable Colorectal Cancer Liver Metastases That Have Failed Chemotherapy 4Phase III Randomised Controlled Trial Confirms Clinical Efficacy and Safety of Radioembolisation Using 90Y-resin Microspheres for Patients With Inoperable Colorectal Cancer Liver Metastases That Have Failed Chemotherapy 5Phase III Randomised Controlled Trial Confirms Clinical Efficacy and Safety of Radioembolisation Using 90Y-resin Microspheres for Patients With Inoperable Colorectal Cancer Liver Metastases That Have Failed Chemotherapy 6Phase III Randomised Controlled Trial Confirms Clinical Efficacy and Safety of Radioembolisation Using 90Y-resin Microspheres for Patients With Inoperable Colorectal Cancer Liver Metastases That Have Failed Chemotherapy 7
(Date:9/23/2014)... has secured $21 million in a Series C Financing. ... Company,s cancer immunotherapy pipeline and advance its lead programs ... round, with participation from Majuven and all existing major ... Venture Capital. , Eureka is engaged in the ... the functions of a T cell receptor, a key ...
(Date:9/23/2014)... majority of drugs used to treat asthma today are the ... are urgently needed to treat this chronic respiratory disease, which ... to wheeze, cough, and find it difficult at best to ... is tough: asthma is a patient-specific disease, so what works ... animal models traditionally used to test new drug candidates often ...
(Date:9/23/2014)... infant chimpanzees from their mothers are well documented, little ... of early life experience. In a year-long study, scientists ... that those who were removed from their mothers early ... performers are likely to show behavioral and social deficiencies ... in the open-access journal PeerJ , was led ...
Breaking Biology News(10 mins):Airway muscle-on-a-chip mimics asthma 2Airway muscle-on-a-chip mimics asthma 3Chimpanzees raised as pets or performers suffer long-term effects on their behavior 2
... days after Hurricane Sandy hit, NJIT Professor Michel Boufadel was ... impact of the storm on the New Jersey shoreline. ... take a team of eight researchers to the beaches of ... five students is still fanning out over the shoreline ...
... in Spanish . U.S. Department of ... against sand flies, which are major pests in Afghanistan, Africa ... vectors of Leishmania parasites that cause leishmaniasis, a devastating disease ... who are bitten by infected sand flies do not know ...
... way foot and mouth disease will be controlled in ... emerging poultry viruses, Two new research projects have ... most devastating livestock and poultry viruses. The two projects, ... (BBSRC), aim to provide novel solutions to combatting the ...
Cached Biology News:NJIT civil engineer receives NSF grant to study storm's impact on Jersey Shore 2Protecting US troops against sand flies 2£12 million funding to tackle devastating livestock and poultry viruses 2£12 million funding to tackle devastating livestock and poultry viruses 3£12 million funding to tackle devastating livestock and poultry viruses 4
Several Pichia pastoris strains are available to allow you to optimize expression and recovery of your protein of interest. Table 1 provides information to help you choose the appropriate strain....
Standard 2" or 3" Plastic can be ordered with 81 or 100 cell dividers. Mini 2" Plastics Boxes come standard with 25 cell dividers. Plastic boxes can also be ordered with corresponding freezer racks...
Request Info...
5-bromouridine 5'-triphosphate (BrUTP) *10 mM in TE buffer*...
Biology Products: