Navigation Links
Optimum FFPE RNA Isolation Kit

respectively.


How can you increase your chances of success with FFPE?
Although the Optimum FFPE Isolation Kit is a robust protocol, there are other uncontrollable factors that can impact the overall quality and yield of RNA isolated from FFPE tissues. Below are a few recommendations to address several key factors.

1) Upstream Tissue Procurement and Tissue Specimen Preparation If possible, tissues should be fixed within one hour of surgical resection. Extensive degradation of RNA can occur before completion of the fixation process. The optimal fixation time is 12-24 hours, using neutral-buffered formalin or paraformaldehyde. Fixed tissues should be thoroughly dehydrated prior to embedding.

2) Tissue Type, Size, and Amount Being Used for RNA Isolation The recommended tissue thickness is 10-20 m (Table 1). The number of sections used is determined by the tissue type (which impacts cell density) and surface area (recommended size: 50-300 mm2). Excess starting material can cause filter clogging, resulting in poor yield.

3) Excessive Amount of Paraffin Used for Embedding Tissues Two times xylene treatment at room temperature should be sufficient for complete deparaffinization. If desired, a more rigorous 37-55C treatment can be done for up to 30 minutes. After the xylene deparaffinization, it is crucial that the 100% ethanol is completely removed and the pellets are d
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. High-Throughput Isolation of Total RNA
3. Simple Isolation of RNA from Tissue and Cultured Cells
4. Fast Isolation of Total RNA from Small Numbers of Cells
5. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
6. Isolation of Microorganisms
7. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
8. Mouse Tail Genomic DNA Isolation Protocol(1)
9. Genomic DNA Isolation Protocol(1,2,3)
10. Basic Plasmid DNA Isolation Protocol
11. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Optimum FFPE RNA Isolation Kit

(Date:10/22/2014)... 2014 Shimadzu Scientific Instruments introduces its ... for the pain management and clinical markets. The software ... review by allowing users to highlight and review results ... QuantAnalytics allows analysts to filter results by group or ... by eliminating the need to sift through analytes of ...
(Date:10/22/2014)... 22, 2014 Currently offering a ... & D Weighing, Rice Lake, and Ohaus, Pipette.com ... . The Sartorius CPA Semi-Micro Balance is ideal ... precise, and user-friendly laboratory balance. , Sartorius is ... their laboratory balances are well known for their ...
(Date:10/22/2014)... According to new market research report ... Integration, Deployment & Support), by Service Model (SaaS, ... Others (Hybrid & Community)) - Global Forecast to ... Cloud Professional Services Market into various sub-segments with ... also identifies the drivers and restraints for this ...
(Date:10/22/2014)... 22, 2014 Nuvilex, Inc. (OTCQB: NVLX) – ... people worldwide are living with diabetes, with  that number ... The global market for diabetes treatments is approximately $500 ... worldwide died from pancreatic cancer.  Pancreatic cancer is the ... in the United States , and ...
Breaking Biology Technology:Shimadzu Releases QuantAnalytics Open Access LCMS Software Package for Clinical Applications 2Pipette.com Launches the Addition of Sartorius CPA Semi-Micro Balance to Their Product Portfolio with Special Promotional Pricing 2Pipette.com Launches the Addition of Sartorius CPA Semi-Micro Balance to Their Product Portfolio with Special Promotional Pricing 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 2Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 3Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 4Cloud Professional Services Market by Public, Private, Hybrid & Community Expected to Reach $34.41 Billion by 2019 - New Report by MarketsandMarkets 5Nuvilex Brief Analyst Report: Thinking Outside the Box by BrokerBank Securities, Inc. 2
... issued on 03/24/09 to NexGen Biomedical, Inc. founder Mark ... of treating lung, brain, pancreatic, breast and colon cancers ... overexpression (also called EGFR or c-ErbB-1). The patent ... / S-Phase cytotoxic protocols achieve only a fraction of ...
... HUNTINGTON BEACH, California and AMSTERDAM, The Netherlands, March ... diagnostics,today announced that the Company is scheduled to ... Biotech Industry" Conference on Thursday,April 2, 2009. Presented ... has built a reputation as a major biotech ...
... SUNNYVALE, Calif. March 24 Cepheid (Nasdaq: ... revolutionize the speed of diagnosis of Mycobacterium tuberculosis ... for the disease.The new test technology, developed in partnership ... University of Medicine and Dentistry of New Jersey ...
Cached Biology Technology:Patent for Making Combination Chemotherapy Work Better 2Cepheid Announces New Diagnostic Technology to Aid World Efforts in Halting the Spread of Mycobacterium Tuberculosis (TB) 2Cepheid Announces New Diagnostic Technology to Aid World Efforts in Halting the Spread of Mycobacterium Tuberculosis (TB) 3Cepheid Announces New Diagnostic Technology to Aid World Efforts in Halting the Spread of Mycobacterium Tuberculosis (TB) 4Cepheid Announces New Diagnostic Technology to Aid World Efforts in Halting the Spread of Mycobacterium Tuberculosis (TB) 5Cepheid Announces New Diagnostic Technology to Aid World Efforts in Halting the Spread of Mycobacterium Tuberculosis (TB) 6
(Date:10/22/2014)... -- Aware, Inc. (NASDAQ: AWRE ), a leading ... for its third quarter ended September 30, 2014. ... an increase of 40% compared to $4.3 million in the ... of 2014 was $4.1 million compared to $1.0 million in ... current three month period was primarily due to: i) a ...
(Date:10/18/2014)... a group of 2,000 patients referred for evaluation ... potential molecular diagnosis for 25 percent, including detection ... new mutations contributing to disease, according to a ... is being released to coincide with the American ... sequencing analyzes the exons or coding regions of ...
(Date:10/17/2014)... . Why do ... to reproduce? And why are there two sexes anyway? These ... of the research journal Molecular Human Reproduction published ... Bielefeld University Bielefeld has compiled this special issue on sperm ... to copulate with several males in quick succession – chimpanzees ...
Breaking Biology News(10 mins):Aware, Inc. Reports Third Quarter 2014 Financial Results 2Aware, Inc. Reports Third Quarter 2014 Financial Results 3Aware, Inc. Reports Third Quarter 2014 Financial Results 4Aware, Inc. Reports Third Quarter 2014 Financial Results 5Aware, Inc. Reports Third Quarter 2014 Financial Results 6Aware, Inc. Reports Third Quarter 2014 Financial Results 7Whole-exome sequencing shows potential as diagnostic tool 2Sperm wars 2
... recently discovered compound from the aminopyridine class not only has ... all strains of malaria, but might also be able to ... to a research collaboration involving the Medicines for Malaria Venture ... Centre (H3-D) at the University of Cape Town, South Africa. ...
... cells perform an array of essential functions, including cell ... and out of cells. They are critical targets for ... as disease biomarkers early warning beacons announcing the ... study of the binding properties of membrane proteins is ...
... Disruptions to the circadian rhythm can affect the growth of ... diabetes, obesity, and cancer, according to a new study from ... is regulated by a "clock" that reacts to both incoming ... in the scientific journal Cell Reports , it is ...
Cached Biology News:African research identifies strong candidate for possible single-dose malaria cure 2African research identifies strong candidate for possible single-dose malaria cure 3A new look at proteins in living cells 2A new look at proteins in living cells 3A new look at proteins in living cells 4
Best pricing for 100mg-1.0g scales. Phosphopeptides are our specialty....
... 2D is an automated, two-dimensional fractionation system ... Part of Beckman Coulters ProteomeLab family ... in just hours. The system effectively ... cell lysates, addressing a key challenge in ...
... The Rapid-Screen cDNA Library Panels were designed ... three sets of PCRs to identify the ... is performed in a 96-well "Master Plate," ... 5,000 clones. Having identified the positive well(s) ...
... HORIBA Jobin Yvons Protein 295 ex ... a protein sample in only a few ... the amino acids tyrosine and tryptophan, the ... powerful NanoLED-17, a pulsed diode emitting 295 ...
Biology Products: