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Optimum FFPE RNA Isolation Kit

Total RNA Isolation from Formalin Fixed Paraffin Embedded (FFPE) Tissues


Phenol-free isolation of total RNA from FFPE tissues
Optimized for qRT-PCR

Optimum FFPE RNA Isolation Kit offers a simple and rapid protocol for phenol-free isolation of total RNA from formalin or paraformaldehyde fixed paraffin embedded (FFPE) tissues that is optimized for RT-PCR analysis. The kit is designed for use with small amounts of sample material, and the Micro Filter Cartridge allows RNA to be eluted in a concentrated form. The kit is also compatible with cells from LCM samples.


Why use FFPE tissues?
FFPE tissues are the most widely available specimens for retrospective clinical studies of disease mechanisms. These archived materials provide a valuable source of stable RNA for gene expression analysis, using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). As the use of PCR technology has become more prevalent in molecular testing, it has enhanced the clinical utility of FFPE tissues; e.g. retrospective analysis of archival tissue would enable the correlation of molecular findings with the response to treatment and the clinical outcome.


Why is RNA isolation from FFPE such a challenge?
The recovery of quality RNA from FFPE specimens can be quite challengi
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