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Optimizing pfuturbo DNA Polymerase Amplification Reactions with,,,Perfect Match PCR Enhancer

ing a third, unrelated amplification system designed to synthesize a 120-bp fragment from human genomic DNA. In the absence of Perfect Match enhancer, both PfuTurbo DNA polymerase and Taq DNA polymerase produce multiple undesired side products. Adding Perfect Match enhancer significantly decreases the synthesis of larger, undesired side products by both enzymes. Unlike amplifications of long complex targets (Figure 1 and Figure 2), PfuTurbo and Taq DNA polymerases synthesized similiar yields of the relatively short amplicon.

Conclusions

Perfect Match enhancer provides a quick, convenient solution for reducing amplification of undesired PCR side products. Use Perfect Match PCR enhancer to improve the yield and specificity of difficult PCR amplification systems employing both PfuTurbo DNA polymerase and Taq DNA polymerase. The distinct PCR-enhancing activities of Perfect Match enhancer and the Turbo additive work in concert with Pfu DNA polymerase to provide high product yields with minimal PCR artifacts and replication errors. Perfect Match PCR enhancer may be purchased separately or as part of Stratagenes Opti-Prime PCR optimization kit. This kit consists of a set of buffers and PCR-enhancing agents (including formamide, DMSO, and Perfect Match PCR enhancer) for use in an easy, two-step protocol to optimize difficult PCR amplification reactions.6

REFERENCES

  1. Hogrefe, H. et al. (1997) Strategies 10: 93-96.

  2. Hogrefe, H., Bai, F., and Cline, J. (1998) Strategies 11: 36-37.

  3. Nielson, K. and Mathur, E.J. (1990) Strategies 3: 17-22.

  4. Lundberg, K.S., Mathur, E.J. (1991) Strategies 4: 4-5.

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