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Optimizing pfuturbo DNA Polymerase Amplification Reactions with,,,Perfect Match PCR Enhancer


Improve PCR product yield and specificity for difficult amplifications

Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer

Frances Bai Holly Hogrefe
Stratagene

Certain PCR amplification systems produce multiple product bands or products of the wrong size due to nonspecific priming events, template secondary structures, or suboptimal PCR conditions. Stratagenes Perfect Match PCR enhancer *, improves the yield and specificity of problematic PCR reactions carried out with Taq DNA polymerase. We investigated the effects of Perfect Match PCR enhancer on amplifications performed with pfuturbo DNA polymerase,**, a robust high-fidelity PCR enzyme recently developed by Stratagene. Using PfuTurbo polymerase, the addition of the enhancer substantially improved the yield and specificity of suboptimal PCR systems; furthermore, PCR product yields and target-length capability were higher than for Taq plus Perfect Match enhancer reactions.

Stratagene is committed to developing innovative, high-performance PCR enzymes and PCR-enhancing agents to maximize product yield, specificity, and fidelity. Stratagene recently introduced PfuTurbo DNA polymerase, a special formulation of cloned Pfu DNA polymerase and a novel thermostable PCR-enhancing factor*** (the Turbo additive) that dramatically improves PCR product yields without altering DNA replication fidelity.1 PfuTurbo DNA polymerase is ideally suited for amplifying genomic DNA targets up to 10 kb and cloned targets up to 15 kb with the greatest accuracy possible.1 PCR comparisons have shown that PfuTurbo DNA polymerase amplifies longer targetsin higher yieldthan Pfu DNA polymerase (alone), Taq DNA polymerase, and other commercially availa
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