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Optimizing electroporation parameters

Other important parameters for optimizing electroporation.
Pulse Length In addition to the field strength, a crucial factor for successful transfection is the pulse length. It is primarily dependent on the diameter of the cell: the larger the cell, the longer the pulse necessary for permeation of the membrane. Empirically, the ideal pulse lengths for electroporation have proven to be 40100 s at room temperature and 1540 s at 4C. To optimize the duration of the pulse, three different pulse lengths should be selected within the above-mentioned ranges.



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