Navigation Links
Optimizing electroporation parameters

Other important parameters for optimizing electroporation.
Influence of temperature

The temperature has a direct effect on the permeation voltage of the cell membrane (see field strength) as well as on the regeneration of the membrane following electroporation.

a) Influence of the temperature on the permeation voltage of the cell membrane:
Since the permeation voltage at 4C is twice that at room temperature (1), it is essential to take the temperature into account when determining the optimal field strength of the pulse. During electroporation at 4C, the necessary field strength of the pulse is nearly twice as high as those values for room temperature. However, mammalian cells are usually electroporated at room temperature.

b) Influence of the temperature on the regeneration of the cell membrane:
Incubating cells following electroporation
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
2. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
3. Optimizing electroporation parameters
4. Optimizing Büchi® Rotary Evaporator Applications
5. Optimizing electroporation parameters
6. Optimizing Büchi® Rotary Evaporator Applications
7. Optimizing Bchi Rotary Evaporator Applications
8. Optimizing electroporation parameters
9. Optimizing electroporation parameters
10. Optimizing electroporation parameters
11. Optimizing electroporation parameters
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Optimizing electroporation parameters

(Date:1/22/2015)... (PRWEB) January 22, 2015 Pipette.com has ... to their comprehensive portfolio of Eppendorf products. , ... to purchase a Centrifuge 5424/5424 R and receive the ... either Eppendorf Research plus or Eppendorf Reference 2 ...
(Date:1/22/2015)... (PRWEB) January 22, 2015 Selexis SA, ... mammalian Research Cell Banks (RCBs) used for drug discovery ... Cell Banks will include Next-Generation Sequencing (NGS) ... architecture de-risks biologic manufacturing by ensuring the integrity of ...
(Date:1/22/2015)... , Jan. 22. 2015  Varian Medical Systems (NYSE: ... been honored for its commitment to sustainability with inclusion on ... is the highest ranked healthcare equipment company among the Corporate ... the World Economic Forum at Davos, Switzerland ...
(Date:12/24/2014)...  United Therapeutics Corporation (NASDAQ: UTHR ) announced ... has submitted a pre-market approval application to the U.S. ... Medtronic,s SynchroMed ® II implantable drug infusion system ... Therapeutics, Remodulin ® (treprostinil) Injection delivered intravenously to ...
Breaking Biology Technology:Eppendorf Announces Promotional Bundle Which Includes: Eppendorf Centrifuge 5424, 3-Pack of Pipettes, and Tips - Available Now at Pipette.com 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2Varian Honored Among World's 100 Most Sustainable Corporations 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 3United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 4
... , The Isopure Buccal Cell DNA Kit is a simple, ... genomic DNA for downstream applications , such as ... collection formats using SPRI magnetic bead technology , ... , Buccal Kit effectively and consistently delivers high ...
... Agencourts RNAClean kit provides a simple, flexible and ... products generated in common enzymatic , RT and ... , gene expression analysis experiments and is easily ... eliminates the need for vacuum filtration or , ...
... Although the <A , ... Labeling Kits were developed for labeling siRNA, they can , ... can be used for this application. See "<A , ... Introduced dsRNA in Cells " for experimintal data using dsRNA , ...
Cached Biology Technology:Isopure - Buccal cell DNA isolation 2RNAClean - RNA & cDNA in vitro Reaction Purification 2Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit 2Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit 3Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit 4
(Date:12/17/2014)... SM announced today its completion of a Good ... current U.S. Food and Drug Administration (FDA) regulations. This ... smart phone application trials utilizing the highest principles for ... determined to improve global healthcare access, quality, and delivery ...
(Date:12/11/2014)... Markets , http://photos.prnewswire.com/prnh/20130307/600769 ... addition of the "Biometrics Market in the APAC ... One major trend emerging in this market is advances ... important to upgrade biometric solutions to the latest standard ...
(Date:12/10/2014)... 9, 2014  Valencell, a leader in performance biometric data ... demand from its licensees for highly accurate, clinically validated ... solely coming from fitness and health sectors, but first ... wearable is only as useful as the biometric data ...
Breaking Biology News(10 mins):HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2Wearable Technology Products Demand Highly Accurate Biometric Technology 2
... feeds on a native tree from the western Pacific island of ... are out of reach for its only local natural enemy," said ... ecologist Thomas Marler to study the relationship between the native cycad ... alien insect pest called cycad aulacaspis scale (CAS). The pest arrived ...
... comparative analysis to date of bacterial communities inhabiting the ... into why some individuals become colonized with pathogens while ... in mBio the online open-access journal published by the ... are important sites of pathogen colonization, yet the microbiota ...
... that increasing certain proteins in the blood vessels of ... The study provides new avenues for research that may ... demonstrates that cytochrome P450 plays an important role in ... enormous public health concern," said Darryl Zeldin, M.D., acting ...
Cached Biology News:Cycad pest uses small size to hide from predators 2Study examines, compares bacteria in the nose and throat 2NIH-supported study finds novel pathway may open doors for new blood pressure treatments 2
EDG-1 Western blot control Source: Cell Lysate Application: Western Blotting Control...
...
Kit containing positive control DNA all parts of the HIV-1 genome, HIV-1 negative control DNA, Primers SK38, SK39, SK19...
Request Info...
Biology Products: