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Optimizing electroporation parameters

Other important parameters for optimizing electroporation.
Influence of temperature

The temperature has a direct effect on the permeation voltage of the cell membrane (see field strength) as well as on the regeneration of the membrane following electroporation.

a) Influence of the temperature on the permeation voltage of the cell membrane:
Since the permeation voltage at 4C is twice that at room temperature (1), it is essential to take the temperature into account when determining the optimal field strength of the pulse. During electroporation at 4C, the necessary field strength of the pulse is nearly twice as high as those values for room temperature. However, mammalian cells are usually electroporated at room temperature.

b) Influence of the temperature on the regeneration of the cell membrane:
Incubating cells following electroporation
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