Navigation Links
Optimizing electroporation parameters

Other important parameters for optimizing electroporation.
Influence of the DNA/RNA The transfection efficiency of electroporation can be affected by the concentration, the purity and the size of the molecules used.

a) Influence of nucleic acid concentration:
With the optimal electroporation parameters (osmolarity, voltage, pulse length), the quality of the results obtained with plasmid concentrations between 5 g/ml and 20 g/ml is usually satisfactory. The efficiency of the transfection may be raised by increasing the nucleic acid concentration, but only within a limited concentration range. Tests with various different cell lines have shown that only in very few cases (e.g. when large plasmids were used) do plasmid concentrations in excess of 20 g/ml lead to an increase in the transfection rate (see Fig. 1.).


'"/>

Source:


Page: All 1 2

Related biology technology :

1. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
2. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
3. Optimizing electroporation parameters
4. Optimizing Büchi® Rotary Evaporator Applications
5. Optimizing electroporation parameters
6. Optimizing Büchi® Rotary Evaporator Applications
7. Optimizing Bchi Rotary Evaporator Applications
8. Optimizing electroporation parameters
9. Optimizing electroporation parameters
10. Optimizing electroporation parameters
11. Optimizing electroporation parameters
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Optimizing electroporation parameters

(Date:7/10/2014)... June 17, 2014 Using microscopic polymer light ... gases, researchers at MIT,s Quantum Photonics Laboratory have ... in the parts-per-billion range. Optical sensors are ideal ... high signal-to-noise ratio, compact, lightweight nature, and immunity ... sensors had been developed before, the MIT team ...
(Date:7/10/2014)... extremely challenging because there are many genetic variants that ... Now investigators reporting in the July 3rd issue of ... describe a strategy that may help reveal how such ... environmental exposures to affect the development of the nervous ... predispose individuals to schizophrenia. , The work takes ...
(Date:7/10/2014)... our laptops and electric vehicles could store more energy ... help of a sponge-like silicon material. , Researchers ... used in one of the battery,s electrodes, as silicon ... of graphite. A paper describing the material,s performance as ... Nature Communications . , "Silicon has long been sought ...
(Date:7/10/2014)... shelled out for crude 3-D glasses, polarized glasses, ... These basic devices, used to trick the brain ... be rendered obsolete with the introduction of new ... , TAU doctoral students Yuval Yifat, Michal Eitan, ... based on nanoantennas that could be used for ...
Breaking Biology Technology:Swell new sensors 2New strategy could uncover genes at the root of psychiatric illnesses 2Silicon sponge improves lithium-ion battery performance 2Silicon sponge improves lithium-ion battery performance 3Projecting a 3-dimensional future 2Projecting a 3-dimensional future 3
... Trubion Pharmaceuticals, Inc. (Nasdaq: TRBN ) ... SCORPION(TM) multispecific therapeutic technology at the 100th Annual ... (AACR) in Denver. The data presented demonstrated the ... SCORPION molecule, its differentiation from monospecific approaches, and ...
... demonstrates company,s commitment to sustainabilityLAS VEGAS, April 22 ... commitment to protecting tomorrow today through its sustainability ... Region for Bayer CropScience, presented Tom Lovelace, United ... million endowment over the next four years to ...
... Cohen to be inducted into National Academy of Sciences for ... Sir Philip Cohen of Scotland,s University of Dundee ... (NAS) during its 146th annual meeting on Saturday, April 25 ... in original scientific research. Sir Philip is one of only ...
Cached Biology Technology:Trubion Presents Preclinical Data on Its SCORPION(TM) Multispecific Therapeutics at the 2009 American Association of Cancer Research Annual Meeting 2Trubion Presents Preclinical Data on Its SCORPION(TM) Multispecific Therapeutics at the 2009 American Association of Cancer Research Annual Meeting 3Trubion Presents Preclinical Data on Its SCORPION(TM) Multispecific Therapeutics at the 2009 American Association of Cancer Research Annual Meeting 4Bayer CropScience Donates $1.1 Million to United Fresh Research & Education Foundation to Create the Center for Global Produce Sustainability 2Bayer CropScience Donates $1.1 Million to United Fresh Research & Education Foundation to Create the Center for Global Produce Sustainability 3Bayer CropScience Donates $1.1 Million to United Fresh Research & Education Foundation to Create the Center for Global Produce Sustainability 4United States Recognizes Leading Scottish Scientist for Disease Research 2
(Date:7/10/2014)... In Santa Fe, Albuquerque, and other major cities in ... watered with treated municipal wastewater rather than precious potable ... more than 40% of all golf courses receive treated ... courses. , Additionally, golf courses and homeowners alike ... nutrient in fertilizer is nitrate. A New Mexico State ...
(Date:7/10/2014)... 2014  The American Academy of Forensic Sciences ... and Technology (NIST) and the Department of Justice ... forensic science expert members to the Forensic Science ... of the NIST,s Organization of Scientific Area Committees ... appointments made to the new FSSB that has ...
(Date:7/10/2014)... 2014  Pomerantz LLP has filed a class ... the "Company")(NYSE-MKT: PVCT) and certain of its officers.  ... Court, Middle District of Tennessee ... class consisting of all persons or entities who ... 17, 2013 and May 22, 2014, both dates ...
Breaking Biology News(10 mins):'Tailored' water -- the latest in lawn care 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 2Ten Members of the American Academy of Forensic Sciences Appointed to Forensic Science Standards Board (FSSB) 3SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 2SHAREHOLDER ALERT: Pomerantz Law Firm Announces the Filing of a Class Action against Provectus Biopharmaceuticals, Inc. and Certain Officers - PVCT 3
... cell phones have dramatically changed the way we share the ... friends and family. Now, the imaging sensor chips that form ... the California Institute of Technology (Caltech) transform the way cell ... "smart" petri dish. Dubbed ePetri, the device is ...
... D.C.-- The world,s largest celebration of science and ... return to Washington, D.C., April 28-29, 2012. For ... will participate with hands-on experiments. At Carnegie,s booth ... a real diamond produced by our high pressure ...
... Ore. At the end of the last Ice Age, ... scientists have long hypothesized that the source was CO 2 ... study using detailed radiocarbon dating of foraminifera found in a ... the Northeast Pacific was not an important reservoir of carbon ...
Cached Biology News:Caltech engineers build smart petri dish 2Caltech engineers build smart petri dish 3Carnegie partners with USA Science & Engineering Festival for second year 2Carnegie partners with USA Science & Engineering Festival for second year 3Rising CO2 levels at end of Ice Age not tied to Pacific Ocean 2Rising CO2 levels at end of Ice Age not tied to Pacific Ocean 3
casein kinase 2, alpha 1 polypeptide, transcript variant 2, mRNA (cDNA clone MGC:15232 IMAGE:4121882), complete cds...
...
UV LAMP 300 NM 8W T5, 1 EA. Category: Nucleid Acid Detection Systems....
MOUSE ANTI S. MUTANS PEPTIDOGLYCAN...
Biology Products: