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Optimizing electroporation parameters

at which the cell membrane is permeated. The minimum pulse voltage is dependent on the cell diameter following incubation in electroporation buffer, as well as on the temperature, and on the gap width of the cuvettes. The values shown can be used to determine the ideal pulse voltage to be set on the device.

Figure 2. Cells swell up under hypoosmolar conditions. In this state, electroporation can be performed under milder pulse conditions.

Example:

Cell type: Suspension cells

Cell diameter in electroporation medium: ~ 20 m

Gap width of cuvette: 2 mm

Temperature in the cuvette: Room temperature

Minimum pulse voltage according to diagrams (Fig. 1): ~ 130 V

Series of experiments for optimizing pulse voltage: 130 V / 260 V / 390 V



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