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Optimizing electroporation parameters

width="20%">120 240 50 50 100 100 210 60 40 80 80 160 80 30 60 60 120 Table 1. Minimum pulse voltages at which the cell membranes may be permeated, in relation to the diameter of the cell after a 1015 min incubation in the electroporation buffer, the electroporation temperature and the gap width of the cuvette. Note: Depending on the cell line, the optimal pulse voltage for the electroporation experiment may be between two and five times higher. Figure 1. Minimum pulse voltage
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