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Optimizing electroporation parameters

Other important parameters for optimizing electroporation.
Field Strength The field strength (V/cm) of the electrical pulse used is an essential factor in determining the survival rate, as well as the transfection rate, of the cells used.

If the field strength of the pulse exceeds a specific threshhold (= critical external field strength), reversible permeation occurs in the cell membrane. This so-called permeation voltage is heavily dependent on the temperature at which electroporation takes place. The diagrams in Fig. 1 show the minimum values of the pulse voltage which have to be set in relation to the cell diameter and the temperature at which the electroporation is performed. The diameter of the cell is determined after the cells have been incubated in electroporation buffer for 1015 minutes (see

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