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Optimizing electroporation parameters

TD vAlign=top align=middle>Eppendorf Isoosmolar Electroporation Buffer (PI) (no.: 4308 070.510) Sterile bidistilled water Fill up to 1000 ml Fill up to 1000 ml KCl 25 mM 25 mM KH2PO4 0.3 mM 0.3 mM K2HPO4 0.85 mM 0.85 mM myo-Inositol* ad 90 mOsmol/kg ad 280 mOsmol/kg pH value 7.2 0.1 7.2 0.1 Conductivity at 25C 3.5 mS/cm 10% 3.5 mS/cm 10% Shelf-life 3 years 3 years Table 1: Eppendorf Electroporation Buffer components

*The purity of myo-Inositol may vary greatly from
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