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Optimizing electroporation parameters

Other important parameters for optimizing electroporation.
Adjustment of the electroporation buffer A precondition for successful electroporation with the Multiporator is the special buffer system with low electrical conductivity.

The best possible transfection results are obtained by using the original buffers from Eppendorf, which have been tested for sterility as well as for the absence of mycoplasma, endotoxins and pyrogens, and which have also under-gone a thorough cytotoxicity test.

Users may also make up this buffer themselves (see Table 1).

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