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Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler

(1993). Target selection and optimization of amplification reaction. In: Diagnostic molecular microbiology, 88-102. Eds.: David H. Persing, Thomas F. Smith, Fred C. Tenover, Thomas J. White, ASM.

(3) Hayashi, K. (1994). Manipulation of DNA by PCR. In: PCR - the Polymerase chain reaction, 3-14. Eds.: K.B. Mullis, F. Ferre & R.A. Gibbs, Birkhuser.

(4) Innis, M.A. & Gelfand, DH (1990). Optimization of PCRs. In: PCR Protocols: A guide to methods and applications, 3-11, Academic press.

(5) Charlieu, J.-P. (1994). PCR as a technique used daily in molecular biology. In: PCR Technology, current innovations, 1-4. Eds.: G. Hugh & A.M. Griffin, CRC press.

(6) McConlogue, L., Brow, M.A.D. & Innis, M.A.(1988). Structure independant DNA amplification. Nucleic Acids Res. 16: 9869.

(7) Rolfs, A., Schuller, I., Finckh, U. & Weber-Rolfs, I. (1992). PCR principles and reaction components, 1-18. In: PCR: Clinical diagnostics and research, Springer.

(8) Don, R.H. et al. (1991). "Touch down" PCR to circumvent spurious priming during gene ampification. Nucleic Acids Res. 19: 4008.

(9) Hosta, F. (1991). Enhancement of specifity and yield of PCR. United States Biochemical Corp. Editorial comments Vol. 18, No.3, 1-5.

(10) Cheng, S., Chang, S.-Y., Gravitt, P. & Respess R. (1994). Long PCR. Nature 369: 684-685.

(11) DAquila, R.T., Bechtel, L.J., Videler, J.A., Eron, J.J., Gorczycy, P. & Kaplan, J.C. (1991). Maximizing sensitivity and specificity of PCR by preamplification heating. Nucleic Acids Res. 19: 3749.

(12) Rolfs, A., Schuller, I., Finckh, U. & Weber-Rolfs, I. (1992). Physical features of thermocyclers and their influence on the efficiency
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