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Optimized Imaging of Protein Gels Stained with Coomassie,,,Brilliant Blue Dye

ce of white ABS plastic. This technique of capturing an image takes advantage of the overhead white-light illumination provided inside the cabinet of the Eagle Eye II still video system and can be used by researchers who either do not have a white-light box or do not want to replace the UV transilluminator with the white-light box prior to imaging a gel stained with Coomassie blue dye.

Figure 1A

Figure 1B

Figure 1C

Figure 1A illustrates the comparison of optical filters. Compared to the standard ethidium bromide-optimized optical filter (Figure 1A), the Coomassie blue bandpass filter enhances contrast and improves sensitivity in images of Coomassie blue-stained protein gels (Figure 1B). Sensitivity was further increased when the gel was placed on top of a piece of white ABS plastic, and the Coomassie blue-optimized filter was used in conjunction with overhead white-light illumination (Figure 1C). However, this method also has the potential of increasing nonspecific background. When the ethidium bromide filter was used in conjunction with overhead white-light illumination and a piece of white plastic, sensitivity was increased when compared to the ethidium bromide filter used alone (data not shown). However, the combination of the Coomassie blue bandpass filter, overhead white-light illumination and a piece of white ABS plastic gave the most sensitive results.

Conclusions

The Coomassie blue bandpass filter expands the line of optical filters for the Eagle Eye II sti
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