Navigation Links
Optimized Dengue RT-PCR

le lane 9 is water (template negative) control. Discussion

Lanciotti et al. (1992) designed the primers using prototype dengue virus strains Den-1 (Hawaii), Den-2 (New Guinea C), Den-3 (H-87) and Den-4 (H-241). In addition, they also included 33 virus isolates from different geographical regions to test the efficiency of their designed primers. According to their paper, only a single PCR product of 511 bp in length was amplified. In our study we also detected, in addition to the target PCR product, the presence of another PCR product measuring at 190 bp. In the beginning, we had assumed that this extra band was a non-specific PCR product.

However, by experimenting at various different annealing temperatures ranging from 56.4C to 72C in our PCR by using Eppendorf's Mastercycler gradient, we found that the 190 bp product persisted in all the different annealing temperatures wheras the target PCR product (511 bp) was not detected at 72C.This could indicate that the primers used by Lanciotti et al. (1992) annealed at least at 2 sites (repeat sequence?) for this particular strain (Vietnamese B98). We hope to sequence this strain to identify the genotype.

Reference

Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV (1992). Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. Journal of Clinical Microbiology, 30(3) 545-551


'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
3. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
4. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
5. Brilliant Core Reagent Kits for QPCR and QRT-PCR
6. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
7. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
8. Performance Comparisons of Commercial RT-PCR Systems
9. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
10. RT-PCR Primers for the Study of Apoptosis
11. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Optimized Dengue PCR

(Date:4/27/2015)... 27, 2015   Aratana Therapeutics, Inc . ... focused on the licensing, development and commercialization of ... commentary on clinical study data involving ADXS31-164 (AT-014) ... at the American Association for Cancer Research (AACR) ... novel Lm -LLO immunotherapy developed by Advaxis, ...
(Date:4/27/2015)... HEIDELBERG , Germany , April ... need in treatment of millions of cancer patients   ... a patent in Europe entitled ... Health,s discovery that EPHB4 is a novel receptor for Erythropoietin ... stimulates the production of red blood cells and is ...
(Date:4/27/2015)... 27, 2015 tru Shrimp™ Systems announced ... Texas A&M University for the exclusive worldwide rights to ... raceway systems. The license agreement for the patented technology, ... to countries such as China, Taiwan, France, Germany, Italy, ... “We have had inquires from all over the world ...
(Date:4/27/2015)... , April 27, 2015 Elsevier ... products and services, and Qatar University Research Office   have ... Awards. The awards ceremony, held last week at ... , was attended by more than 40 guests representing ... were presented by Professor Sheikha Abdulla Al-Misnad , President ...
Breaking Biology Technology:Aratana Therapeutics Comments on ADXS31-164 (AT-014) Study Data Presented During Poster Session at the American Association for Cancer Research (AACR) Annual Meeting 2015 2Molecular Health Gains Patent for Novel Receptor Utilizing Computational Biomarker Discovery Technology 2tru Shrimp™ Systems Acquires Global Patent for Tidal Basin™ Shrimp Production 2Elsevier Announces the Winners of Qatar University Scientists 2015 Scopus Awards 2Elsevier Announces the Winners of Qatar University Scientists 2015 Scopus Awards 3Elsevier Announces the Winners of Qatar University Scientists 2015 Scopus Awards 4Elsevier Announces the Winners of Qatar University Scientists 2015 Scopus Awards 5
... Nov. 24 Boston Scientific,Corporation (NYSE: BSX ) ... which is being held December 2 - 3 in New ... will make a 30-minute presentation on,Tuesday, December 2, beginning at ... A live webcast of Mr. Tobin,s remarks will be ...
... CLAREMONT, Calif., Nov. 24 Keck Graduate Institute,s (KGI) Biotechnology ... educational event on Monday, Dec. 8, 2008. , ... (TCA), a network of individual investors who fund early-stage, technology ... of the public interested in becoming angel investors. The ...
... Omrix,s Biopharmaceutical Expertise Complements ETHICON,s Industry-Leading Surgery Portfolio ... N.J. and NEW YORK, Nov. 24 Johnson & ... Inc. (Nasdaq: OMRI ), a fully integrated ... products, today announced a definitive agreement whereby Omrix will ...
Cached Biology Technology:KGI Entrepreneur Program Hosts Angel Investing Educational Meeting 2Johnson & Johnson Announces Definitive Agreement to Acquire Omrix 2Johnson & Johnson Announces Definitive Agreement to Acquire Omrix 3Johnson & Johnson Announces Definitive Agreement to Acquire Omrix 4Johnson & Johnson Announces Definitive Agreement to Acquire Omrix 5
(Date:4/20/2015)... Calif. , April 20, 2015 Huntington ... Gabriel Valley to implant a new miniaturized, wireless monitoring ... System is the first and only FDA-approved heart failure ... hospital admissions when used by physicians to manage heart ... sensor that is implanted in the pulmonary artery (PA) ...
(Date:4/14/2015)... 2015  HYPR Corp. today announced it has ... Alliance tm , an industry consortium transforming online ... share technology and collaborate to deliver open specifications ... secure and private, and easier to use. ... protects sensitive user information and eliminates the dependency ...
(Date:4/13/2015)... 13, 2015  higi, a leading cloud-based consumer engagement ... fully engage with their communities around health and wellness, ... and secure API.  ... accessible, affordable, and convenient vehicle to receive validated health ... The API will allow higi,s trusted partners, ...
Breaking Biology News(10 mins):Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 2Huntington Memorial Hospital Becomes First in the San Gabriel Valley to Offer Patients a New Heart Failure Monitoring Solution 3HYPR Corp. Joins the FIDO Alliance 2HYPR Corp. Joins the FIDO Alliance 3higi Launches Seamless Access to HIPAA-Compliant Health Outcomes and Activity Data 2higi Launches Seamless Access to HIPAA-Compliant Health Outcomes and Activity Data 3
... The Scripps Research Institute (TSRI) have found an easier way to ... new method allows scientists to add a marker to certain cells, ... from a larger cell population. The technique, which is ... Chemie International Edition , makes use of the tight binding of ...
... (RQR), Palais des congrs de Montral, Montral, Qubec, Canada, 22-26 ... 2013 -- The Society for the Study of Reproduction ... the 46th SSR Annual Meeting to be held in Montral, ... Meetings page ( http://www.ssr.org/Meetings.shtml ) for details; information will be ...
... New Rochelle, NY, January 8, 2013The world,s leading zebrafish ... promote science education. Scientific papers, many authored by students ... curricula and educational tools, a collection of abstracts (including ... from the recent Zebrafish in Education Workshops ...
Cached Biology News:Chemists devise inexpensive, benchtop method for marking and selecting cells 2Chemists devise inexpensive, benchtop method for marking and selecting cells 3Surprising teaching tool in K-12 science education -- Zebrafish research 2
HiTrap NHS-activated HP, 1 x 5 ml. Category: Chromatography Systems & Accessories, Systems....
Human Serpin A8/AGT Affinity Purified Polyclonal Ab Keywords: Ang II, AngII, Hypertension, Salt Homeostasis, Pre-eclampsia...
Human CXCL14/BRAK Affinity Purified Polyclonal Ab...
Human/Mouse G9a/EHMT2 MAb (Clone A8620A) Keywords: Eu-HMTase2...
Biology Products: