Navigation Links
Optimize Transfection of siRNAs for RNAi

by the corresponding siRNA, but not by the negative control siRNA. In contrast, little to no mRNA reduction was observed in several of the cell types when suboptimal media conditions were used. For the remainder of the study, COS-7 cells were transfected in serum supplemented media using siPORT Amine Agent.

Figure 1. Effect of Serum on siRNA Transfection. The indicated cells types were transfected with GAPDH siRNA or negative control siRNA using either siPORT Amine or siPORT Lipid Transfection Agent (Ambion) in cell culture media supplemented with (+) or without () serum (10%) for 4 hr. 48 hours after transfection GAPDH mRNA levels were assessed by Northern analysis.


Effect of cell density on transfection of siRNA. To test whether cell density at the time of transfection affects siRNA-mediated mRNA reduction, COS-7 cells were plated in a 24 well dish at different plating densities 24 hours prior to transfection (Figure 2). Transfections were performed with either 10 nM GAPDH siRNA or with a negative control (scrambled) siRNA using 4 l of siPORT Amine Agent. After 48 hr mRNA expression was evaluated by real-time PCR. Reduction in gene expression varied
significantly at different cell plating densities. GAPDH siRNA-mediated reduction in mRNA was maximal at relatively low plating densities between 2.5 and 5 x 104 cells per well; little to no mRNA reduction was observed at higher plating densities (Figure 2). To minimize decreased cell growth associated with very low plating densities, an optimal cell plating density of 3 x 104 cells per well wa
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
3. Optimized Dengue RT-PCR
4. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Optimize Transfection siRNAs for RNAi

(Date:7/30/2015)... , July 30, 2015 AACC ... to the 2015 AACC Annual Meeting & Clinical ... from July 26–30. The meeting showcased revolutionary advancements ... the ability of healthcare providers to diagnose patients ... effective medical treatment. As of ...
(Date:7/30/2015)... VANCOUVER, Washington , July 30, 2015 ... "Biosimulation Market, by Product (Software, Molecular Modeling, PB/PK, PKPD, ... (Drug Discovery & Development, Preclinical, Clinical Trial), by End ... the Biosimulation Market is expected to reach USD 2,107.99 ... at a CAGR of 15.29%. Browse ...
(Date:7/30/2015)... globe, ISN improves the efficiency and effectiveness of contractor management systems. ISN,s online contractor ... in over 80 countries. ... ... Over 60,000 Worldwide Customers In More Than 80 Countries ... Dallas, TX , ISN has additional offices in Calgary , ...
(Date:7/30/2015)... ... 30, 2015 , ... As part of its 2015 growth plan and as a follow up ... business units, Whitehouse Laboratories is pleased to announce that it has begun construction on ... of Excellence and will be strictly dedicated to basic USP 51, USP 61, and USP ...
Breaking Biology Technology:Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 2Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 3Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 4Biosimulation Market Worth 2,107.99 Million USD by 2020 2Biosimulation Market Worth 2,107.99 Million USD by 2020 3Biosimulation Market Worth 2,107.99 Million USD by 2020 4ISN Reaches Over 60,000 Worldwide Customers 2ISN Reaches Over 60,000 Worldwide Customers 3Whitehouse Labs Formally Announces Plans to Open a Microbiological Laboratory 2
... VIENNA, Va., June 4 In response to ... is providing an,update on the construction of its ... built out, will comprise 73,000 square,feet and will ... product. This dedicated facility will produce the Multikine ...
... Research, MELVILLE, N.Y., June 4 ... University,s Feinberg School of,Medicine announced the opening ... a partnership that brings improved research,capabilities to ... development. One of only three such centers ...
... (OTC Bulletin Board: BSTC.OB) today announced that it,sold ... at $13 per,share with aggregate proceeds to BioSpecifics ... group on May 30, 2008. The shares were ... premium over the market price., BioSpecifics Technologies ...
Cached Biology Technology:CEL-SCI CORPORATION Manufacturing Facility for Pivotal Phase III Cancer Trial Scheduled to Be Completed During 2008 Third Quarter 2CEL-SCI CORPORATION Manufacturing Facility for Pivotal Phase III Cancer Trial Scheduled to Be Completed During 2008 Third Quarter 3Nikon Instruments Opens New Imaging Center at Northwestern University 2Nikon Instruments Opens New Imaging Center at Northwestern University 3
(Date:7/30/2015)... MOUNTAIN VIEW, Calif. , July 30, 2015 ... products and services for gene function analysis and ... CRISPR Guide RNA (sgRNA) Knockout Library targeting all ... to specifically and permanently "knock out" a gene,s ... library provides a high throughput screening tool so ...
(Date:7/23/2015)... July 23, 2015 Aware, Inc. (NASDAQ: AWRE ... reported financial results for its second quarter ended June 30, ... 2015 was $4.5 million, a decrease of 33% compared to ... in the second quarter of 2015 was $0.3 million, or ... $0.04 per diluted share, in the same period a year ...
(Date:7/21/2015)... Connecticut , July 21, 2015  NXT-ID, ... "Company"), a biometric authentication company focused on the ... Wocket® smart wallet, announces that it has filed ... System and Method. This invention ... not only authorizes an account, but also the ...
Breaking Biology News(10 mins):CELLECTA, INC. Announces Launch of Human Whole Genome CRISPR Knockout Library 2Aware, Inc. Reports Second Quarter 2015 Financial Results 2Aware, Inc. Reports Second Quarter 2015 Financial Results 3Aware, Inc. Reports Second Quarter 2015 Financial Results 4Aware, Inc. Reports Second Quarter 2015 Financial Results 5Aware, Inc. Reports Second Quarter 2015 Financial Results 6NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 2NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 3NXT-ID Patents Personalized Tokenized Payments for Next Generation Secure Payment Technology 4
... pair of proteins that work in concert to restart the ... The Hawley Lab has described the dual actions ... awakens the long-dormant egg, allowing for production of a mature ... 4, 2007 issue of PLoS Biology. The long ...
... from the John Innes Centre have proven that by ... and delivering it with an existing antibiotic they can ... transfer company PBL, the scientists have launched a spin-out ... The DNA sequence acts as a decoy, disrupting gene ...
... severe weather and climate change joined forces to study ... severe storms in the future and discovered a dramatic ... the United States. The Purdue University-led team used ... formation of severe thunderstorms - those that produce flooding, ...
Cached Biology News:Stowers Institute's Hawley Lab identifies factors responsible for restart of meiotic cycle 2Decoy makes sitting duck of superbugs 2Research forecasts increased chances for stormy weather 2Research forecasts increased chances for stormy weather 3Research forecasts increased chances for stormy weather 4Research forecasts increased chances for stormy weather 5
Monoclonal mouse antibody for immunihistology, clone AD12, Isotype IgG 1, purified, specific for rat pyruvatekinase type L, no cross-reactivities with other pyruvate kinase isoenzymes have been obser...
For testing the AQUA technique on your MS instruments....
A robust formulation of Taq Polymerase supplied with a 10X colored reaction buffer which allows Direct Gel-Loading...
... Dinitro-5,5'-dithiodibenzoic acid , DTNB , Ellman's reagent ... , *CAS-No.: 69-78-3 *Storage: RT, ... a reactive grouping not present in the substrate, ... which will react directly with the product to ...
Biology Products: