Navigation Links
Optimize Transfection of siRNAs for RNAi

aximizing the performance of siRNA in tissue culture. We discuss the use of chemical transfection agents and offer suggestions on how to optimize critical transfection parameters.


Important Parameters in siRNA Transfection Experiments
Cells vary greatly with respect to their capacity to be transfected. Hence, different transfection reagents and cell culture conditions need to be tested for each cell type. What works for one cell type won't necessarily work for another. Some cells perform best when transfected in serum supplemented media while others require serum free conditions for maximal transfection efficiency. Healthy cells transfect better than poorly maintained cells. Routinely subculturing cells before they become overcrowded or unhealthy will minimize instability in continuous cell lines from experiment to experiment. Since cells may gradually change in culture, using cells within a defined passage number and maintaining strict protocols, including parameters for intervals between plating and transfecting cells, will improve experimental reproducibility.

Cell confluency. An important factor for obtaining a high transfection efficiency is cell density at the time of transfection. For most adherent cells, the optimal confluency for transfection is 30-70%. Suboptimal cell density (too low or too high) can result in poor uptake of the siRNA:transfection agent complexes and insufficient silencing of the gene of interest.

Choice of transfection agent. The overall tran
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. Eagle Eye Optical Filter Optimized for Imaging Silver-Stained Gels
3. Optimized Dengue RT-PCR
4. Rapamycin Optimized in 100 Liter Fermentor Using BioCommand Control Strategys
5. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
6. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
7. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
8. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
9. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
10. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
11. Mycoplasma Contamination Reduces the Effect of Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Optimize Transfection siRNAs for RNAi

(Date:9/18/2014)... 18, 2014  MEI Pharma, Inc. (Nasdaq: MEIP ... novel therapies for cancer, announced today that Daniel P. ... at BioCentury,s NewsMakers in the Biotech Industry conference ... from the Millennium Broadway Hotel & Conference Center in ... presentation can be accessed at www.meipharma.com . A replay ...
(Date:9/18/2014)... ST. LOUIS , Sept. 18, 2014 /PRNewswire/ ... ) announced today it has entered into a ... Michigan (U-M) Medical School,s Vector Core. Under the ... Sigma CRISPR technology, experimental design consultation, and dedicated ... poised to accelerate gene editing-based research at the ...
(Date:9/18/2014)... Now a team led by an engineer at Michigan ... that will let scientists slash the cost of one ... Syringe pumps are used to dispatch precise amounts of ... a reaction. They can also cost hundreds or even ... of Michigan Tech students published the library of free ...
(Date:9/18/2014)... The quest for the ultimate memory device for computing ... at The City College of New York led by ... exhibit both magnetic and ferroelectric properties. , Combining both ... can occur between them and for the devices that ... Combining these two properties in a single material, however, ...
Breaking Biology Technology:MEI Pharma To Present At BioCentury's NewsMakers Conference 2MEI Pharma To Present At BioCentury's NewsMakers Conference 3Sigma-Aldrich, University of Michigan Medical School Establish CRISPR Core Lab Partnership 2Sigma-Aldrich, University of Michigan Medical School Establish CRISPR Core Lab Partnership 3Sigma-Aldrich, University of Michigan Medical School Establish CRISPR Core Lab Partnership 4Doing science just got cheaper -- and faster 2Oxides discovered by CCNY team could advance memory devices 2
... few months, 2004 delivered some promising advances in Wisconsin technology. ... has set in motion public initiatives to help entrepreneurs and ... Technology Network just turned two. To celebrate, here are some ... leading CIOs ,Wisconsins CIOs continue to push IT forward ...
... realm of content management systems (CMS) providers, Northwoods ... for clients. Forget the gearhead techno-speak. The Milwaukee area ... issues through plain talk and better Web design. , ... Web, said Pat Bieser, founder and CEO of the ...
... agencies have backed a new organization, the Wisconsin Angel ... , ,Wisconsin excels in researching and developing cutting-edge technology ... first and most critical stage, said Governor ... , ,The Wisconsin Technology Council , which advises ...
Cached Biology Technology:2004 in review: Editors picks 22004 in review: Editors picks 3Untangling the Web: Northwoods Software in profile 2Untangling the Web: Northwoods Software in profile 3Untangling the Web: Northwoods Software in profile 4Untangling the Web: Northwoods Software in profile 5
(Date:9/17/2014)... has received a $260,000 grant from the Andrew W. ... the Human in the Age of the Life Sciences." ... events aimed at exploring two compelling themes in the ... are exploring the boundaries of our self-definition as a ... involves the increasingly influential hypothesis that we are living ...
(Date:9/17/2014)... EC-FP5 four-year project, delivering its first release in 2004. ... in several EC projects, as a part of the ... BON), to increase the general awareness of the work ... dissemination of the Fauna Europaea results, the ... e-Publishing tools to prepare data papers for all 56 ...
(Date:9/17/2014)... cleverly, some lessons on how to create the features ... director of the Optical Technology Center at Montana State ... nanoscale, we,ll discover them." , Some of those lessons ... conference called "The Nature of Light: Light in Nature" ... of Arizona College of Optical Sciences. The conference was ...
Breaking Biology News(10 mins):Mellon Foundation awards grant for major project in the humanities and sciences 2Contributions on Fauna Europaea: Data papers as innovative model on expert involvement 2Nature's designs inspire research into new light-based technologies 2Nature's designs inspire research into new light-based technologies 3
... of birds reproduce not by rearing their own young, but ... Known as brood parasitism, this habit has been most thoroughly ... the nests of martins and swallows in Europe are rarely ... the Hainan Normal University in China and his colleagues suggests ...
... research team has identified seven new gene loci linked to obesity. ... cause extreme obesity are similar to those that cause milder forms ... people were included in the study of the links between genes ... Nature Genetics . The aim of the study was to ...
... 2013, Cleveland: A compound abundant in red meat and added ... to promote atherosclerosis or the hardening or clogging of ... this week in the journal Nature Medicine . ... digestive tract metabolize the compound carnitine, turning it into trimethylamine-N-oxide ...
Cached Biology News:Birds find ways to avoid raising cuckoos' young 2Genes behind obesity mapped in large-scale study 2Cleveland Clinic researchers discover new link between heart disease and red meat 2Cleveland Clinic researchers discover new link between heart disease and red meat 3
When added to either StemSep CD4+ or CD8+ T Cell Enrichment cocktails, human HLA-DR TAC is designed to deplete human HLA-DR from samples to isolate resting CD4+ or CD8+ T cells....
When added to either StemSep CD4+ or CD8+ T Cell Enrichment cocktails, human CD69 TAC is designed to deplete human CD69 from samples to isolate resting CD4+ or CD8+ T cells....
This kit is designed to select cells labeled with phycoerythrin- (PE-) conjugated antibodies. Kit includes reagents for labeling 3 x 10 9 cells and EasySep magnet....
No-Weigh Dithiothreitol (DTT) ,7.7 mg DTT/Tube x 48 tubes...
Biology Products: