Navigation Links
Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

yeast are transformed with M10 and M17 tungsten or 1.0 mm gold microparticles (Table 3). This reduced transformation efficiency is most likely due to the lower velocity of the microparticles at longer travel distances.

Microparticle size also has a significant effect on transformation efficiency, probably for different reasons, however. In the case of cauliflower stem sections, when the optimum particle accelerator parameters were used, the 1.6 mm gold and M17 tungsten (median diameter 1.1 mm) microparticles produced more GUS-expressing cells than did the smaller 1.0 mm gold and M10 tungsten (median diameter 0.7 mm) microparticles. Furthermore, using the 1.6 mm gold microparticles, significant GUS expression was obtained when the cauliflower sections were bombarded at level 4. This may be attributable to the larger size of the 1.6 mm gold microparticles compared to the other microparticles assayed; the velocity of the larger microparticles is less affected by the travel distance. On the other hand, in the case of yeast transformation, using optimum particle accelerator parameters, the M17 tungsten microparticles were slightly better than the 1.0 mm gold and M10 tungsten microparticles in producing ura+ transformants. However, almost no transformants were found when the 1.6 mm gold microparticles were used. This effect is most likely the result of the larger 1.6 mm gold microparticles causing significant damage to the yeast cells (average diameter 6 mm) upon penetration. Because of the heterologous size distribution of the tungsten microparticles, their biological effect is due to a subset of small diameter microparticles.

The final parameter which should be verified in optimizing B
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17

Related biology technology :

1. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
2. PCR Optimization
3. PCR Optimization Kit
4. The Flexible matriXarray Chip System: A New Perspective for Probe Optimization in Gene-Expression Profiling
5. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
6. The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
7. Simple Approaches for Optimization of RT-PCR
8. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
9. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
10. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
11. Comparison of Performance Characteristics of Different Biolistic Devices
Post Your Comments:
(Date:8/30/2014)... 2014 The Global and China ... in-depth study on the current state of the ... , The report provides a basic overview of ... structure. Global market analysis and Chinese domestic market ... developments, trends and competitive landscape of the market. ...
(Date:8/29/2014)... (PRWEB) August 29, 2014 Pittcon is ... MacDonald, and colleague Koichiro Matsuda, Horiba Scientific, have organized ... September 5, 2014. JASIS , Asia’s largest analytical ... 2014, in Makuhari Messe, Japan. , The four ... for Bioanalysis from Single Molecules to Single Cells” will ...
(Date:8/29/2014)... (PRWEB) August 29, 2014 A major ... the biotech start-up dense realm of Boston-Cambridge, is gaining ... able investors. James Sherley, the Director of Boston’s Adult ... the local and national visibility of his company an ... 2013. , In addition to a social media marketing ...
(Date:8/28/2014)... Lawrence, MA (PRWEB) August 28, 2014 “This ... single test to screen from 0 to 150 ppb,” said ... test is a significant benefit to plant owners and USDA-GIPSA ... corn and other commodities. Testing can take place in a ... Not only can the plant test the feed and grain ...
Breaking Biology Technology:Electrical Insulating Varnish Market World, China Regional Analysis & 2020 Forecasts Available at DeepResearchReports.com 2Electrical Insulating Varnish Market World, China Regional Analysis & 2020 Forecasts Available at DeepResearchReports.com 3Fifth Annual U.S. Symposium at JASIS Organized by Pittcon’s Program Chairman Hub MacDonald 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 3The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 4
... nation combats an,alarming rise in chronic diseases, the ... a new report on the next,generation of medicines ... patients., The report found that America,s pharmaceutical ... Michigan - are,testing a record 633 new biotech ...
... ... Bioprocess Industry, ELKTON, Md., ... -- an online interactive design tool that,provides step-by-step instructions for ... STA-PURE(SM) Bioprocess Configurator is unique to the,bioprocess industry. Available at ...
... on Track, SAN MATEO, Calif., Sept. 24 ... company focused on developing and,commercializing novel pain management ... to the European Medicines Agency,s (EMEA) Day 120,questions ... in peripheral neuropathic pain., NeurogesX, MAA for ...
Cached Biology Technology:New Report on 633 Biotechnology Medicines Under Development Released in Michigan 2New Report on 633 Biotechnology Medicines Under Development Released in Michigan 3Gore's Legacy of Innovation Extends From Products to Processes 2Gore's Legacy of Innovation Extends From Products to Processes 3NeurogesX Responds to EMEA's Day 120 Questions 2NeurogesX Responds to EMEA's Day 120 Questions 3
(Date:8/29/2014)... Some species of marine phytoplankton, such as the prolific ... vitamin B1 (thiamine), researchers have discovered. The finding contradicts ... many other eukaryotic microbes depend on scarce supplies of ... really different way to think about the ocean," says ... The ISME Journal paper with CIFAR fellows ...
(Date:8/29/2014)... of the Tibetan plateau -- the largest topographic anomaly ... both its profound effect on climate and its reflection ... GSA Bulletin , Katharine Huntington and colleagues employ a ... modern and fossil snail shells to investigate the uplift ... , Views range widely on the timing of surface ...
(Date:8/28/2014)... have identified a handful of bacterial culprits that may ... and ulcerative colitis, using patients, own intestinal immune responses ... 28 in the journal Cell . , Trillions ... plays a critical role in the development and progression ... number of bacterial species affect a person,s susceptibility to ...
Breaking Biology News(10 mins):Not all phytoplankton in the ocean need to take their vitamins 2Not all phytoplankton in the ocean need to take their vitamins 3Snails tell of the rise and fall of the Tibetan Plateau 2Snails tell of the rise and fall of the Tibetan Plateau 3Snails tell of the rise and fall of the Tibetan Plateau 4Snails tell of the rise and fall of the Tibetan Plateau 5Snails tell of the rise and fall of the Tibetan Plateau 6Snails tell of the rise and fall of the Tibetan Plateau 7Yale study identifies possible bacterial drivers of IBD 2
... coral bleaching prediction system indicates that there will be ... the event will probably not be severe. NOAA issued ... the 11th International Coral Reef Symposium in Ft. Lauderdale, ... a risk of widespread bleaching in the Northwestern Hawaiian ...
... Mass., July 10, 2008 -- The National Institutes of ... to Williams College Assistant Professor of Biology Lara D. ... or CMT. CMT is the most common inherited ... 2,500 individuals. CMT usually shows dominant inheritance and symptoms ...
... a million electrical signals pulsing through your body. At the ... the ion channel. A new study by researchers from ... a meter in ion channels. This movement is critical to ... close in response to changes in voltage across the membrane. ...
Cached Biology News:New NOAA coral bleaching prediction system calls for low level of bleaching in Caribbean this year 2Researchers catch ion channels in their opening act 2Researchers catch ion channels in their opening act 3
Human RXR beta/NR2B2 MAb (Clone H7341) Keywords: RXRbeta, RXRbeta, Retinoid X Receptor beta...
PSMD1 Antibody...
Terrific Broth, 1 kg. Mixture of casein peptone, yeast extract, K2HPO4, and KH2PO4.Used at 47 g/l for E. coli growth with higher cell densities. Category: Nucleotides & Enzymes & Biochemicals, Ultrap...
... 1200L sets the new standard for ... incorporating the latest technologies and providing ... analytical needs. The Atmospheric Pressure ... Quadrupole Analyzer provide superior selectivity and ...
Biology Products: