Navigation Links
Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

s. An example of this type of experiment, in which the macrocarrier travel distance was varied along with the helium pressure and target distance, is shown in Table 4. Analysis of the three variables indicates that the maximum number of transformants is obtained with the sample at level 2 and the helium pressure between 900 and 1,500 psi. The macrocarrier travel distance had little effect on either increasing or decreasing the transformation efficiency. Similar results were found when the gap width was varied as a function of the helium pressure and target distance; neither increasing nor decreasing the gap width affected the number of transformants produced (results not shown). These results were further verified by changing either the gap width or the macrocarrier travel distance under conditions which were optimal for transformation (M17 tungsten microparticles, target at level 2, and helium pressure of 1,100 psi). Again, there was no significant effect on the number of yeast transformants (results not shown).


Discussion
Transient expression of the GUS gene in cauliflower stem sections and stable transformation of a yeast ura auxotroph with the wild type URA gene were used as model systems to identify those bombardment parameters which are important for optimization of Biolistic transformation. The parameters and their optimum conditions are summarized in Table 5 for both systems. In both cases, the most important parameter to optimize is target distance; there is a significant decrease in expression when the target level is changed from the optimum of 6 cm. This is apparent when cauliflower sections are transformed with M17 tungsten microparticles (Table 1) and when
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17

Related biology technology :

1. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
2. PCR Optimization
3. PCR Optimization Kit
4. The Flexible matriXarray Chip System: A New Perspective for Probe Optimization in Gene-Expression Profiling
5. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
6. The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
7. Simple Approaches for Optimization of RT-PCR
8. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
9. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
10. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
11. Comparison of Performance Characteristics of Different Biolistic Devices
Post Your Comments:
(Date:7/30/2015)... --  Senomyx , Inc. (NASDAQ: SNMX ), a ... develop, and commercialize novel flavor ingredients for the food, ... for the second quarter 2015. "Moving ... track to achieve our commercial and financial goals," stated ... Company. "Since our last quarterly earnings report, our two ...
(Date:7/30/2015)... , July 30, 2015  Discovery Laboratories, ... it has received the second $1.0M tranche under ... Research (SBIR) grant valued at up to $3.0 ... aerosolized KL4 surfactant as a potential medical countermeasure ...  Discovery Labs was awarded an initial $1.0 million ...
(Date:7/29/2015)... , July 30, 2015 ... santé, publie ses résultats pour le premier semestre ... commente les résultats. Visionner l,interview vidéo ... http://www.eurobusinessmedia.com/ceo-direct/sanofi/sanofi-h1-2015-results-interview-with-ceo-olivier-brandicourt?utm_source=ceo-direct&utm_medium=wire Au sommaire de l,interview ... - Moteurs de croissance - Diabète ...
(Date:7/29/2015)... 2015 /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ) today ... Financial Officer has resigned.  The Company has hired ... retired in December 2014, as full time interim CFO ... Andy Ashworth began his employment ... smooth transition.  Andy was the Company,s CFO from September ...
Breaking Biology Technology:SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 2SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 3SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 4SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 5SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 6SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 7SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 8SENOMYX ANNOUNCES SECOND QUARTER 2015 FINANCIAL RESULTS 9Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 2Discovery Labs Receives $1.0 million under an SBIR Grant in Continuing Support of the Development of Aerosolized KL4 Surfactant to Address Radiation-Induced Lung Injury 3BioAmber Announces Change in Chief Financial Officer 2BioAmber Announces Change in Chief Financial Officer 3
... thrive in today,s,competitive and changing marketplace, sales force ... doesn,t just happen. Productive,companies train, structure, deploy, and ... to ensure high success rates., Best Practices ... sales forces to evolve and mature to capture ...
... Opportunities -, BRIDGEWATER, N.J. and NEW YORK, June ... announced,its launch backed by Warburg Pincus, the leading global ... in GANIC from Warburg,Pincus Private Equity X, L.P., a ... Pharmaceuticals was founded by a highly seasoned group of ...
... a,global point-of-care diagnostic test manufacturer and wholly owned,subsidiary ... today announced,that its HemoCue Albumin 201 System is ... and monitoring microalbuminuria to be,granted a CLIA waiver ... With,the FDA CLIA waiver, non-laboratory trained physicians and ...
Cached Biology Technology:Increasing Sales Productivity Through Superior Sales Force Performance Management 2GANIC Pharmaceuticals Launched with Premier Pharmaceutical Industry Executives 2GANIC Pharmaceuticals Launched with Premier Pharmaceutical Industry Executives 3HemoCue Receives First FDA CLIA Waiver in Diagnostics Industry for Quantitative Point-of-Care Test for Microalbuminuria, a Kidney and Cardiovascular Disease Risk Marker 2HemoCue Receives First FDA CLIA Waiver in Diagnostics Industry for Quantitative Point-of-Care Test for Microalbuminuria, a Kidney and Cardiovascular Disease Risk Marker 3HemoCue Receives First FDA CLIA Waiver in Diagnostics Industry for Quantitative Point-of-Care Test for Microalbuminuria, a Kidney and Cardiovascular Disease Risk Marker 4HemoCue Receives First FDA CLIA Waiver in Diagnostics Industry for Quantitative Point-of-Care Test for Microalbuminuria, a Kidney and Cardiovascular Disease Risk Marker 5
(Date:7/21/2015)... GOTHENBURG, Sweden , July 21, 2015 ... sensor FPC1025. Already received as well as expected revenues in 2015 ... included in FPC,s communicated revenue guidance of approximately 2,200 MSEK for ... is a leading smartphone manufacturer in China ... selected FPC1 02 5 for Axon ...
(Date:7/13/2015)... OXFORD, Conn. , Jul. 13, 2015 /PRNewswire/ ... the "Company"), a biometric authentication company focused on ... it has filed provisional patent 62/188684 for ... NXT-ID achieves another new convenient and secure method ... expands new, innovative payment methods, introduced with its ...
(Date:7/8/2015)... YORK , July 8, 2015 Summary ... globally, and the fourth most fatal, with a mortality ... The poor prognosis of pancreatic cancer patients has highlighted ... treatment, which is not being met by the current ... an array of products with varying molecule types and ...
Breaking Biology News(10 mins):FPC's Touch Fingerprint Sensor FPC1025 in ZTE's Smartphone Axon 2NXT-ID Patents Behavior-Directed Payments 2NXT-ID Patents Behavior-Directed Payments 3NXT-ID Patents Behavior-Directed Payments 4Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 2Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 3
... team of Montral researchers at the IRCM led by Dr. ... team at Duke University in the United States, discovered that ... by affecting certain types of cognitive performance such as anxiety, ... scientific journals Proceedings of the National Academy of Sciences ...
... simple experiment, a scientist exposes rats to a certain ... symptoms remarkably similar to those seen in Parkinson,s patients. ... disease, which affects 7 million to 10 million people ... Chemical & Engineering News , the weekly newsmagazine of ...
... science team led by researchers at Scripps Institution of Oceanography ... the impact of fishing on a group of fish known ... in the journal Proceedings of the Royal Society B ... conservation targets to protect and preserve fragile coral reefs. ...
Cached Biology News:An important discovery related to anxiety disorders and trauma 2An important discovery related to anxiety disorders and trauma 3Scripps leads first global snapshot of key coral reef fishes 2Scripps leads first global snapshot of key coral reef fishes 3
Recombinant Viral CCI/Fc Chimera, CF...
... DeStreak Reagent, 1 ml. Improves ... gels by preventing streaking.Eliminates extra ... of proteins.Stabilizes proteins throughout entire ... well-resolved protein patterns.Ideal for both ...
LIVE/DEAD® Cell-Mediated Cytotoxicity Kit *for animal cells* *2000 assays*...
SlowFade Antifade Kit with DAPI...
Biology Products: