Navigation Links
Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

s. An example of this type of experiment, in which the macrocarrier travel distance was varied along with the helium pressure and target distance, is shown in Table 4. Analysis of the three variables indicates that the maximum number of transformants is obtained with the sample at level 2 and the helium pressure between 900 and 1,500 psi. The macrocarrier travel distance had little effect on either increasing or decreasing the transformation efficiency. Similar results were found when the gap width was varied as a function of the helium pressure and target distance; neither increasing nor decreasing the gap width affected the number of transformants produced (results not shown). These results were further verified by changing either the gap width or the macrocarrier travel distance under conditions which were optimal for transformation (M17 tungsten microparticles, target at level 2, and helium pressure of 1,100 psi). Again, there was no significant effect on the number of yeast transformants (results not shown).


Discussion
Transient expression of the GUS gene in cauliflower stem sections and stable transformation of a yeast ura auxotroph with the wild type URA gene were used as model systems to identify those bombardment parameters which are important for optimization of Biolistic transformation. The parameters and their optimum conditions are summarized in Table 5 for both systems. In both cases, the most important parameter to optimize is target distance; there is a significant decrease in expression when the target level is changed from the optimum of 6 cm. This is apparent when cauliflower sections are transformed with M17 tungsten microparticles (Table 1) and when
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17

Related biology technology :

1. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
2. PCR Optimization
3. PCR Optimization Kit
4. The Flexible matriXarray Chip System: A New Perspective for Probe Optimization in Gene-Expression Profiling
5. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
6. The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
7. Simple Approaches for Optimization of RT-PCR
8. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
9. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
10. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
11. Comparison of Performance Characteristics of Different Biolistic Devices
Post Your Comments:
(Date:8/31/2015)... , Aug. 31, 2015 FLX Bio, ... development of novel cancer immunotherapies, announced today that it ... Chief Executive Officer and William Ho , M.D., ... join an experienced management team led by veterans of ... , Ph.D., Chief Scientific Officer; Jay Powers , ...
(Date:8/31/2015)... FL (PRWEB) , ... August 31, 2015 , ... ... bring together the world's most successful food and beverage processors, equipment manufacturers and ... competencies in product inspection and industrial weighing and formulation for manufacturers of bakery ...
(Date:8/28/2015)... DUBLIN , Aug. 28, 2015 ... announced the addition of the "Global & USA ... offering. The highly awaited US Biosimilar market ... highly awaited filigrastim biologic, Zarxio. How will the global ... The author reveals that biosimilars could save the US ...
(Date:8/28/2015)... 28, 2015 /PRNewswire/ - RepliCel Life Sciences Inc. ... company focused on the development of autologous cell ... ended June 30, 2015, corporate highlights, and near-term ... are available at www.sedar.com ,  www.edgar.com ... "RepliCel expects to launch its RCS-01 treatment ...
Breaking Biology Technology:FLX Bio Announces Senior Management Hires and Initiation of Phase 1 Trial of FLX925 2FLX Bio Announces Senior Management Hires and Initiation of Phase 1 Trial of FLX925 3FLX Bio Announces Senior Management Hires and Initiation of Phase 1 Trial of FLX925 4METTLER TOLEDO Hosts Food Safety Educational Center at 2015 PROCESS EXPO 2Global & USA BioSimilar Market Analysis Report 2015-2021 2Global & USA BioSimilar Market Analysis Report 2015-2021 3RepliCel Reports Second Quarter 2015 Financial Results 2RepliCel Reports Second Quarter 2015 Financial Results 3RepliCel Reports Second Quarter 2015 Financial Results 4RepliCel Reports Second Quarter 2015 Financial Results 5
... University of California, San Diego electrical engineers are building a ... solar energy without using fossil fuels and harvest it for ... the team said nanowires, which are made from abundant natural ... way to deliver hydrogen fuel on a mass scale. ...
... March 7, 2012 Neuralstem, Inc. (NYSE Amex: ... cells in the cervical (upper back) region of the spine ... trial of its spinal cord neural stem cells in amyotrophic ... also the first woman to be treated in the trial. ...
...   Camelot Healthcare Training Institute , a post-secondary nursing ... has been acquired by Medtech College.  Camelot now joins ... College once appropriate state approvals have been obtained. ... campuses offering accredited training in nursing and a number ...
Cached Biology Technology:Nanotrees harvest the sun's energy to turn water into hydrogen fuel 2Fourteenth Patient Dosed in Neuralstem ALS Stem Cell Trial 2Fourteenth Patient Dosed in Neuralstem ALS Stem Cell Trial 3Camelot Healthcare Training Institute Acquired by Medtech 2Camelot Healthcare Training Institute Acquired by Medtech 3
(Date:8/24/2015)... 2015  Based on its recent analysis of the biometric ... African Biometrics Company of the Year Award. DERMALOG is the ... in Africa . It has particularly gained ... implemented one of the largest biometric mass applications in the ... Central Bank of Nigeria with its ...
(Date:8/20/2015)... OXFORD, CT ., Aug. 20, 2015 ... "Company"), a biometric authentication company focused on the growing ... wallet, announces it has joined The Smart Card Alliance ... the Smart Card Alliance and the Smart Card Alliance ... and visibility to industry peers and thought leaders promoting ...
(Date:8/18/2015)... DUBLIN , Aug. 17, 2015 ... the addition of the "Global Biometric Authentication ... Application - Estimation & Forecast, 2015-2020" report ... biometric authentication and identification systems market is expected ... from 2015 to 2020 and generate over $25 ...
Breaking Biology News(10 mins):Frost & Sullivan Applauds DERMALOG's Rise as the Supplier of Best-in-class Biometric Solutions in Africa 2Frost & Sullivan Applauds DERMALOG's Rise as the Supplier of Best-in-class Biometric Solutions in Africa 3Frost & Sullivan Applauds DERMALOG's Rise as the Supplier of Best-in-class Biometric Solutions in Africa 4Frost & Sullivan Applauds DERMALOG's Rise as the Supplier of Best-in-class Biometric Solutions in Africa 5Frost & Sullivan Applauds DERMALOG's Rise as the Supplier of Best-in-class Biometric Solutions in Africa 6NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 2NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 4Global Biometric Authentication & Identification Market 2015: Focus On Modality, Motility & Application - Estimation & Forecasts to 2020 for the $25 Billion Industry 2
... when cosmetics, shampoos, skin creams, and other personal care ... to plant-derived extracts and other natural ingredients government ... the silicone-based ingredients long used in these products. That,s ... of Chemical & Engineering News (C&EN), ACS, weekly newsmagazine. ...
... Weizmann Institute scientists have added another piece to the obesity ... active in a small part of the brain contributes to ... . Prof. Ari Elson and his team in ... with female mice that were genetically engineered to lack this ...
... A group of 12 biology educators at ... argues in the May issue of BioScience ... and teaching of ecology conducted at these institutions. ... dedicated to continental-scale observations, argues that better coordination ...
Cached Biology News:Weizmann Institute scientists discover: A protein that contributes to obesity 2
sPLA2-XII Human, Rabbit Polyconal Antibody Anti-Human sPLA2-XII Antibody was raised in rabbits against Human sPLA2-XII His-Tagged Fusion Protein Antigen: The recombinant human protein sPLA-XII...
... computing power of your ValveBank system! Program ... PC computer running Windows 98, 2000, XP using ... to disk, print out program listings, then download ... simple step., EasyCode is used for programming before ...
Porcine Aortic Smooth Muscle Cells (PAOSMC) (>500,000 cells)...
Bovine Renal Artery Smooth Muscle Cells (BRASMC) (>500,000 cells)...
Biology Products: