Navigation Links
Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

ures of either 900 or 1,300 psi, changing the gap width or macrocarrier travel distance had very little effect on transient expression. These experiments indicate that for Biolistic transformation of cauliflower sections using the PDS-1000/He system, optimum transient expression is obtained when the sample is placed at level 2 (6 cm target distance) and bombarded at a helium pressure of 900 psi with either M17 tungsten or 1.6 mm gold microparticles.

Stable transformants can not be isolated from cauliflower tissue sections. However, these transient assays may be used as starting conditions for optimizing stable transformation. A rigorous experiment for resolving the optimal conditions for isolating stable transformants could be determined in a fractional factorial experiment as outlined in Table 4. Such an experiment should be undertaken only if experimental quantitation is relatively simple. A more practical approach would be to vary those parameters which have the greatest effect on transient expression, in this case helium pressure and target level. In optimizing for stable transformation, the most severe Biolistic conditions which are used should be the mildest conditions which produce a high level of transient expression (e.g., helium pressure of 900 psi and target at level 2). Optimization of stable transformation should use lower helium pressures and/or longer target distances than conditions for transient expression. (Also see comments in the Discussion section.) Since similar levels of GUS expression were obtained using M17 tungsten and 1.6 mm gold microparticles, it may be preferable to use gold microparticles for producing stable transformants due to the demonstrated toxicity of tung
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17

Related biology technology :

1. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
2. PCR Optimization
3. PCR Optimization Kit
4. The Flexible matriXarray Chip System: A New Perspective for Probe Optimization in Gene-Expression Profiling
5. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
6. The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
7. Simple Approaches for Optimization of RT-PCR
8. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
9. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
10. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
11. Comparison of Performance Characteristics of Different Biolistic Devices
Post Your Comments:
(Date:9/16/2014)... Toronto, Canada (PRWEB) September 16, 2014 ... Estate, the Toronto affiliate of ITRA Global, new development ... With the Toronto city council approving another 755 storeys ... buildings), it appears construction cranes will be dominating the ... that seems to have an unquenchable thirst for new ...
(Date:9/15/2014)... EnGeneIC, Ltd. , an emerging ... cancer through the targeted delivery of therapeutic agents ... the Asbestos Disease Research Institute (ADRI), it has ... (NSW) Premier,s Award for Excellence in Translational Cancer ... that will be involved in the clinical trial, ...
(Date:9/15/2014)... researchers led by Northeastern University has developed a ... and a variety of nanocarbon structures in carbon ... scalable, which will allow the researchers to tailor ... in applications ranging from electronic devices to CNT-reinforced ... sports equipment. , Their findings were published in ...
(Date:9/15/2014)... AUSTIN, Texas , Sept. 15, 2014 The ... changes in climate, says a new article published by the ... Ahmed , PhD, on climate change and its effects on ... part of an extensive study conducted by Dr. Ahmed in ... China and has implications for the future ...
Breaking Biology Technology:ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 2ITRA Global Reports Downtown Toronto Real Estate Development is in High Gear 3EnGeneIC Named a Recipient of the Cancer Institute NSW Premier's Award for Excellence in Translational Cancer Research 2EnGeneIC Named a Recipient of the Cancer Institute NSW Premier's Award for Excellence in Translational Cancer Research 3Northeastern University Researchers Develop Novel Method for Working with Nanotubes 2Tea Taste and Quality Affected by Climate Change 2Tea Taste and Quality Affected by Climate Change 3Tea Taste and Quality Affected by Climate Change 4
... Evaluating KRX-0401 in Advanced Renal Cell Carcinoma, Colorectal Cancer ... NEW YORK, May 18 Keryx Biopharmaceuticals, Inc. (Nasdaq: ... on KRX-0401 (Perifosine), the Company,s Akt-inhibitor for cancer, were ... the 45th Annual Meeting of the American Society of ...
... Elderly and Patients With Movement Disorders at Home and ... Millions of people in the US have diseases ... many of them, traditional walking aids such as canes ... to walk normally and live independently.Now there,s a high-tech ...
... Scientific Advisory Board Member Recognized for Development of Statistical ... Wong, Professor of Statistics and of Health Research & ... to the US National Academy of Sciences. Election ... the highest honors that can be accorded to a ...
Cached Biology Technology:Keryx Biopharmaceuticals Announces Poster Presentations Highlighting Clinical Activity of KRX-0401 (Perifosine) at the Upcoming Annual Meeting of the American Society of Clinical Oncology 2Keryx Biopharmaceuticals Announces Poster Presentations Highlighting Clinical Activity of KRX-0401 (Perifosine) at the Upcoming Annual Meeting of the American Society of Clinical Oncology 3Keryx Biopharmaceuticals Announces Poster Presentations Highlighting Clinical Activity of KRX-0401 (Perifosine) at the Upcoming Annual Meeting of the American Society of Clinical Oncology 4Now Available: State-of-the-Art Gait Training Device to Help Millions Walk More Normally Again 2Professor Wing Wong Elected to the National Academy of Sciences 2
(Date:9/16/2014)... impact that spelled doom for the dinosaurs 66 million ... to a much greater extent than their deciduous peers, ... results are published in the journal PLOS Biology ... trove of thousands of fossilized leaves of angiosperms ... able to reconstruct the ecology of a diverse plant ...
(Date:9/16/2014)... vessel returned to Spain culminating the around the world ... picture of how the global ocean works and what ... from the atmosphere is not limited to coastal areas, ... the planet, and it is already affecting the ocean ... in CSIC Residence for Researchers in Barcelona, in a ...
(Date:9/16/2014)... CRG researchers shed ... Journal of Cell Biology describes how Topo 2 disentangles ... At this very moment thousands of our body,s cells are ... body repairs damaged tissues and regenerates others like skin and ... during which the cell duplicates its genetic material and separates ...
Breaking Biology News(10 mins):Meteorite that doomed dinosaurs remade forests 2Meteorite that doomed dinosaurs remade forests 3Meteorite that doomed dinosaurs remade forests 4The Malaspina Expedition confirms that pollution reaches even the most remote areas of the ocean 2The Malaspina Expedition confirms that pollution reaches even the most remote areas of the ocean 3Unraveling cell division 2Unraveling cell division 3
... microarray that holds 1,200 individual cultures of fungi or ... to a study published in mBio , the ... Scientists at the University of Texas at ... Research at Fort Sam Houston have developed a microarray ...
... 24, 2013 /PRNewswire-iReach/ -- TabletKiosk®, a leading manufacturer ... today announces a new collaboration with SensoMotoric Instruments ... control, guidance and registration technology, to cooperatively develop ... PC with market ready eye tracking integration. ...
... This news release is available in ... Parentage-based tagging (PBT) is an emerging genetic-based fish tagging method ... approach to stock identification because the parents, not the offspring, ... This method provides the same information as traditional physical tags ...
Cached Biology News:New palm-sized microarray technique grows 1,200 individual cultures of microbes 2TabletKiosk and SMI Collaborate to Produce the Sahara EyeSlate 2TabletKiosk and SMI Collaborate to Produce the Sahara EyeSlate 3It's all in the genes -- including the tracking device 2
... The steadylite plus assay ... High assay sensitivity, Designed for ... Suitable for higher density microplates ... plates, Odor free, Convenient storage ...
Sf21 cells derived from Spodoptera frugiperda ovarian cells are recommended for high-level production of recombinant proteins. Higher expression levels of some proteins are seen due to the larger ce...
... assay system offers the following ... for batch-processing systems, Long lasting ... microplates such as 384-well and ... storage conditions ( 2 - ...
Sf9 cells derived from Spodoptera frugiperda ovarian cells (Sf21 cells) are commonly used to isolate and propagate recombinant AcMNPV viral stocks and to produce recombinant proteins....
Biology Products: