( microcarriers and transfer them to the...)Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

microcarriers and transfer them to the center of a macrocarrier. An effort is made to remove equal amounts (500 mg) of microcarriers each time and to spread them evenly over the central 1 cm of the macrocarrier using the pipette tip. Desiccate immediately.

References
Armaleo, D., Ye, G.-N., Klein, T. M., Shark, K. B., Sanford, J. C. and Johnston, S. A., Biolistic nuclear transformation of Saccharomyces cerevisiae and other fungi, Curr. Genet., 17, 97-103 (1990).

Daniell, H., Krishnan, M. and McFadden, B. F., Transient expression of β-glucuronidase in different cellular compartments following Biolistic delivery of foreign DNA into wheat leaves and calli, Plant Cell Rep., 9, 615-619 (1991).

Hill, J. E., Myers, A. M., Koerner, T. J., and Tzagoloff, A., Yeast/ E. coli shuttle vectors with multiple unique restriction sites, Yeast, 2, 163-167 (1986).

Jefferson, R. A., Kavanagh, T. A. and Bevan, M. W., GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants, EMBO J., 6, 3901-3907 (1987).

Johnston, S. A., Anziano, P. Q., Shark, K., Sanford, J. C. and Butow, R. A., Mitochondrial transformation in yeast by bombardment with microprojectiles, Science, 240, 1538-1541 (1988).

Kempthorne, O., The Design and Analysis of Experiments, R. E. Krieger Publishing Co., Malabar, Florida (1983).

Klein, T. M., Fromm, M., Weissinger, A., Tomes, D., Schaaf, S., Sletten, M. and Sanford, J. C., Transfer of foreign genes into intact maize cells with high-velocity microprojectiles, Proc. Natl. Acad. Sci. USA, 85, 4305-4309 (1988a).

Klein, T. M., Harper, E. C., Svab, Z., Sanford, J. C
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