Navigation Links
Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System

iolistic transformation is the helium pressure. While the fungal and plant experiments reported here show that the range over which optimum transformation occurs is quite broad (there is little difference in transformation efficiency between 900 and 1,550 psi), this is not always the case. In other systems, such as the stable transformation of bacteria (Smith, et al., 1992) or plants (Russell, et al., 1992a), the optimum helium pressure range is narrower. Because microparticle bombardment will result in cell damage, the most gentle conditions which give a high transformation efficiency should be used. This is particularly true when transient gene assays are performed as preliminary experiments for determining conditions to use in generating stable transformants (see below).

The experimental results obtained here indicate that two of the parameters which can be varied on the PDS-1000/He instrument, the gap distance and the macrocarrier travel distance, may have little effect on the transformation efficiency. Varying either of these parameters and subsequently assaying their effect on yeast transformation (Table 2) or transient expression in cauliflower (Table 4) failed to show a significant effect. Table 6 summarizes the effect of changing the particle accelerator parameters on microparticle velocity and transformation efficiency.

Biolistic transformation of yeast and cauliflower stem sections has not been reported previously using the PDS-1000/He instrument. However, transformation of yeast and other plant tissues has been reported using the gunpowder-driven PDS- 1000 unit. Armaleo, et al. (1990) described optimized biological parameters for yeast, and recommended using 1.1 mm tungsten micropa
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17

Related biology technology :

1. Two-dimensional Optimization of a Semi-nested PCR for Detecting Listeria monocytogenes
2. PCR Optimization
3. PCR Optimization Kit
4. The Flexible matriXarray Chip System: A New Perspective for Probe Optimization in Gene-Expression Profiling
5. Rapid Chromatography Optimization by pH Scouting Using the New BioLogic Maximizer Valve System, Rev A
6. The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
7. Simple Approaches for Optimization of RT-PCR
8. Mobile Phase Optimization for the Analysis of an Antibody-Based Fusion Protein and Aggregates using a TSKgel Super SW3000 Size-Exclusion Column
9. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
10. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
11. Comparison of Performance Characteristics of Different Biolistic Devices
Post Your Comments:
(Date:10/30/2014)... Ras Al Khaimah, UAE (PRWEB) October 30, 2014 ... the appointment of Dr. James A. Manganello, Chairman of the ... (NSCEC) of The Bahamas. The appointment was made by ... The Bahamas on September 24th, 2014. , More ... Cell Therapy and Research Bill , Prime Minister Perry Christie ...
(Date:10/30/2014)... in the development of quantum technologies has been proposed ... published today (28 October) in Nature Communications , ... a new type of flexibly designed microscopic trap for ... and specialised superfast computers, often depend on harnessing the ... these tiny particles are hugely problematic because of the ...
(Date:10/27/2014)... 27, 2014 The Italian ... exhibit and sell its artificial "lower limb", after ... patent.      (Photo: http://photos.prnewswire.com/prnh/20141027/713097 ... fighting, the definitive judgement has arrived: Roadrunnerfoot, the ... and accessible hi-tech prostheses with composite materials, has ...
(Date:10/27/2014)... MA (PRWEB) October 27, 2014 ... profit. SoundConnect , an industry leading unified ... its partners and agents to deliver cloud-based audio ... accelerate growth opportunities. , With SoundConnect’s Q4 ... every twenty-five video and/or web conferencing licenses sold, ...
Breaking Biology Technology:Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 2Grace Century’s Stem Cell Biobank Project Announces Chairman’s Appointment to National Stem Cell Ethics Committee of The Bahamas 3Italian Lower Limb Prosthesis Company Wins Lawsuit Against German Giant Ottobock 2SoundConnect Unveils Q4 Partner Incentives 2
... will be celebrated with a special ceremony that features H.R.H. ... Kavli laureates. Acclaimed scientists in the fields of astrophysics, nanoscience ... The award ceremony, lectures and symposia are open to media. ... September 9, in Oslo Concert Hall prior to the ceremony, ...
... LAS VEGAS, Aug. 26 Desert Radiologists, Nevada,s,largest ... that five,new physicians have joined the team of ... years., (Logo: http://www.newscom.com/cgi-bin/prnh/20050322/DRLOGO ), ... 1995 when he graduated,from the University of Pennsylvania ...
... Cell,Culture, Life Science Research and Diagnostics Industry Adds Seasoned Sales, ... ... Safety and Time to Market, FORT COLLINS, Colo., ... of animal free,products that enhance productivity, safety and time to ...
Cached Biology Technology:Inaugural Kavli Prize ceremony and symposia: Sept. 8-11 (Oslo, Norway) 2Inaugural Kavli Prize ceremony and symposia: Sept. 8-11 (Oslo, Norway) 3Desert Radiologists Welcomes Five New Physicians to the Practice 2Desert Radiologists Welcomes Five New Physicians to the Practice 3InVitria Expands Sales Team 2InVitria Expands Sales Team 3
(Date:10/28/2014)... from North America, Europe and China have published a ... of Sciences that reveals important details about key ... planet., From strange and exotic algae, mosses, ferns, trees ... grains and vegetables we eat and the ornamental plants ... over a billion years of history., "Our study generated ...
(Date:10/28/2014)... be building a hybrid micro-grid which will integrate ... in the region, the hybrid micro-grid will test ... diesel, storage and power-to-gas technologies, and ensure these ... built under the new Renewable Energy Integration Demonstrator- ... located offshore at Semakau Landfill and is expected ...
(Date:10/27/2014)... This news release is available in German . ... rapidly propagate information in their interior via electrical signals, ... known as the synapses. Chemical messenger substances, the neurotransmitters, ... synapse becomes active, some of these vesicles fuse with ... that valuable time is not lost, synapses always have ...
Breaking Biology News(10 mins):New study uses DNA sequences to look back in time at key events in plant evolution 2New study uses DNA sequences to look back in time at key events in plant evolution 3NTU to build region's first renewable energy integration demonstration micro-grid 2NTU to build region's first renewable energy integration demonstration micro-grid 3Synapses always on the starting blocks 2Synapses always on the starting blocks 3
... brain imaging techniques, researchers at Georgetown University Medical Center have ... to learn novel tasks, an advance they say may help ... offer strategies to retrain people with perceptual deficits due to ... , the research team provides the first human evidence for ...
... An artificial nose could be a real benefit at ... explosives or drugs, for instance. Researchers at the Max ... Institute of Biochemistry recently revealed a technique for integrating ... important functions in the cell, one of which is ...
... from the University of Pittsburgh Cancer Institute's Center for ... contain substances that mimic the actions of estrogen, the ... and can concentrate chemicals from their habitat within their ... making their way into the region's waterways. , ...
Cached Biology News:Using brain scans, researchers find evidence for a two-stage model of human perceptual learning 2Using brain scans, researchers find evidence for a two-stage model of human perceptual learning 3Instruction manual for creating a molecular nose 2What's in the water? Estrogenic activity documented in fish caught in Pittsburgh's rivers 2
ANTI-HUMAN CYTOKINES, GROWTH FACTORS (polyclonal rabbit (or goat) antibodies) Human TARC - Biotin...
Mouse monoclonal [7C6] to Bcr-abl ( Abpromise for all tested applications). SwissProtID: P11274...
Rabbit polyclonal to Glutathione S Transferase alpha ( Abpromise for all tested applications). Antigen: Full length protein: Human Glutathione S Transferase alpha. Entrez GeneID: 2938 Swi...
Chicken polyclonal to Thioredoxin domain containing 9 ( Abpromise for all tested applications). Antigen: Full length protein (Human) Entrez Gene ID: 10190 Swiss Protein ID: O14530...
Biology Products: