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Omniscript RT Kit

For standard reverse transcription Omniscript Reverse Transcriptase is specially designed for all reverse transcription with any amount of RNA from 50 ng to 2 g per reaction. A higher affinity for RNA results in higher sensitivity and superior performance even with low copy numbers (see figure "Sensitive RT-PCR of =10 Copies"). Omniscript RT is also usually the enzyme of choice with viral RNA due to the presence of carrier RNA in most viral RNA preparations. In comparative experiments, Omniscript RT consistently outperforms other reverse transcriptases over a wide range of starting RNA amounts (see figures"Comparison of Various Reverse Transcriptases" and "Sensitive RT-PCR of =10 Copies"). Sensitive RT-PCR of =10 Copies A 700 nt in vitro transcript was reverse transcribed from 10 to 104 copies of starting template (in 200 ng total RNA) followed by PCR. 1/4 of each reverse-transcription reaction (corresponding to 2.5 to 2500 cDNA copies) was used for PCR amplification of a 330 bp fragment.



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