ts necessary to perform oligonucleotide
separations using the BioFocus capillary electrophoresis system:
Two fused silica capillaries, coated internally with poly(AAEE),
30 cm 75 m I.D. 375 m O.D., with detection
window 8 cm from the end
CE Oligonucleotide Run Buffer, 50 ml
CE Oligonucleotide Size Standards 8-32mer, 13 peaks, total 0.5
OD units (see Figure 1).
Purity Results Fast
The BioFocus system provides automated analysis of up to 28 samples in
one carousel setup. As Figure 2 illustrates, separations can be achieved
in 10 or 12 minutes. The BioFocus Integration Software can be programmed
to automatically integrate the results and print a quantitative report.
Accurate Size Estimation
The size standard can be used as an external standard to estimate the
size of the sample oligonucleotide. Figure 3 shows an overlay of an 8mer
synthysis product and the CE Oligonucleotide Size Standard.
Figure 2 shows an electropherogram of the CE Oligonucleotide Size Standard.
Figure 4 shows that typical peak efficiency for the 32mer component is
above 200,000 theoretical plates. Single base resolution is usually achievable
out to 40mer. Resolution decreases with increasing size of the oligonucleotide.
Table 1 shows the excellent reproducibility of the technique. Over a series
of 12 successive runs, the average %RSD for area is 1.54% and 0.82% for
Page: All 1 2 3 Related biology technology :1
Fast Results for a given sample are available in 12 minutes
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in Microarray Analysis