Navigation Links
Oil-Free PCR Using the Hot Top Assembly

b>

Duplicate cycle sequencing reactions were set up and performed under identical cycling parameters on a RoboCycler 96 temperature cycler. The first set of reactions was performed with the standard paddle and an oil overlay. The standard paddle was then removed and replaced with the Hot Top Assembly. The second set of reactions was prepared with the oil overlay omitted and cycled using the Hot Top Assembly. After cycling, equal volumes of the sequencing products were electrophoresed on a sequencing gel using the CastAway system.6 The gel was fixed, rinsed with water, dried and exposed to film. When either the standard paddle or the Hot Top Assembly was used, equivalent cycle sequencing results were obtained (figure 3). Similar results were observed when the experiment was performed using a RoboCycler 40 temperature cycler (data not shown).

Conclusions

By heating PCR tubes from both the top and sides, the Hot Top Assembly for RoboCycler temperature cyclers dramatically reduces the amount of condensation formed during cycling and eliminates the need to add oil or wax overlays to reaction tubes. The Hot Top Assembly makes postcycling manipulations, such as gel loading, significantly faster and easier, especially when many samples are analyzed. Our results demonstrate that the addition of the Hot Top Assembly to RoboCycler units did not compromise the quality of PCR or cycle sequencing results when compared to those obtained using a standard paddle and an oil overlay. By installing the new software upgrade PC card, which comes with the Hot Top Assembly for the RoboCycler 96 unit, any RoboCycler 96 temperature cycler can be retrofitted to become an oil-free PCR machine. Hot Top Assemblies are now available for any RoboCycler 96 or RoboCycler 40 temperature cycler,
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
3. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
4. Using XL1-Red Mutator Strain to Generate Esterase Variants
5. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
6. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
7. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
8. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
9. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
10. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
11. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Oil Free PCR Using the Hot Top Assembly

(Date:3/27/2015)... LONDON , March 27, 2015 ... Royal Free Hospital in London , Richmond ... for a global Phase 3 study for an investigational RNAi ... an orphan disease affecting the nerves and heart. ... Record Detail Based at St Georges University ...
(Date:3/27/2015)... Governor Andrew M. Cuomo today announced ... - the world’s largest business idea competition with $5 ... $1 million, six $500,000 awards and four $250,000 awards. ... compete for prizes that will turn ideas with high ... York region. , "Last year's successful 43North competition ...
(Date:3/26/2015)... SQI Diagnostics Inc. ("SQI" or the "Company") (TSX-V: SQD; ... including the appointment of auditors, its annual and special ... "Meeting") was adjourned, as previously disclosed on March 25, ... Toronto time). The date of the ... The Meeting will reconvene at the offices of the ...
(Date:3/26/2015)... and CINCINNATI , March ... (LSE: SHP, NASDAQ: SHPG ) and Cincinnati ... broad research collaboration for rare diseases. The goal of ... therapies to treat rare diseases with high unmet ... with Cincinnati Children,s research expertise. As a nationally ...
Breaking Biology Technology:Governor Andrew M. Cuomo Anounces Launch of Year Two of World's Largest Business Idea Competition and Opening of New Incubator Space in Buffalo, New York 2Governor Andrew M. Cuomo Anounces Launch of Year Two of World's Largest Business Idea Competition and Opening of New Incubator Space in Buffalo, New York 3Governor Andrew M. Cuomo Anounces Launch of Year Two of World's Largest Business Idea Competition and Opening of New Incubator Space in Buffalo, New York 4Governor Andrew M. Cuomo Anounces Launch of Year Two of World's Largest Business Idea Competition and Opening of New Incubator Space in Buffalo, New York 5SQI Diagnostics Inc. Announces Adjournment of Meeting 2Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 2Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 3Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 4Shire and Cincinnati Children's Establish Rare Disease Research Collaboration 5
... - Asian populations with regard to risk associated in the ... osteoporosis or cardiovascular disease, ... in response to nutritional ... WALTHAM, Mass., Feb. 28 Interleukin Genetics,Inc. (Amex: ILI ...
... Transgenomic Inc. (OTC,Bulletin Board: TBIO) announces the following Webcast:, What: ... Conference Call, When: ... http://www.transgenomic.com/events.asp?id=6 , How: ... address above., ...
... ANDS ) today announced that patient dosing has ... oral TLR7 agonist prodrug, in,patients with advanced solid tumors. ... to identify pharmacologically active doses,and preliminary antitumor activity as ... II trials., The Phase I trial is a ...
Cached Biology Technology:Interleukin Genetics Enters into New Clinical Research Collaboration with Alticor 2Interleukin Genetics Enters into New Clinical Research Collaboration with Alticor 3Interleukin Genetics Enters into New Clinical Research Collaboration with Alticor 4Interleukin Genetics Enters into New Clinical Research Collaboration with Alticor 5Anadys Pharmaceuticals Commences Phase I Clinical Trial of ANA773 in Cancer Patients 2Anadys Pharmaceuticals Commences Phase I Clinical Trial of ANA773 in Cancer Patients 3Anadys Pharmaceuticals Commences Phase I Clinical Trial of ANA773 in Cancer Patients 4
(Date:3/4/2015)... Research and Markets ( http://www.researchandmarkets.com/research/48tj8z/global_biometrics ... "Global Biometrics Market Forecast and Opportunities, 2020" ... for biometric authentication systems is projected to grow ... The driving forces for this highly ... projects and constant development in technology. In 2014, ...
(Date:3/2/2015)... , March 2, 2015 Synaptics Inc. ... of human interface solutions, today announced a new ... designed to enable rapid and secure transactions and ... for gaming is a turnkey solution providing mouse ... to quickly integrate fingerprint ID solutions into their ...
(Date:3/2/2015)... --  Neurotechnology , a provider of high-precision biometric, robotics ... Development Kit (SDK). The SentiGaze algorithm enables designers ... to track eye movements and generate heatmaps based on ... be used for applications such as analysis of websites ... can also be used to develop interfaces for a ...
Breaking Biology News(10 mins):Global Biometrics Market Forecast and Opportunities, 2020 2Global Biometrics Market Forecast and Opportunities, 2020 3Synaptics Unveils New Fingerprint ID Solution for Gamers 2Synaptics Unveils New Fingerprint ID Solution for Gamers 3Neurotechnology Releases SentiGaze SDK for Eye Movement Tracking Using Ordinary Webcams 2Neurotechnology Releases SentiGaze SDK for Eye Movement Tracking Using Ordinary Webcams 3
... (March 30, 2011)The Society of Interventional Radiology Foundation,s Discovery ... invasive medicine into new areas of discovery, announced a ... provider of medical devices for vascular access and the ... in Marlborough, Mass., has pledged $500,000 and was named ...
... German . , Innovations relating to mobility, energy, ... at the 2011 Hannover Messe from April 4 8. ... a hybrid Porsche racing car, developments in battery research, materials ... European energy supply system, and the Energy Solution Center (EnSoC) ...
... blood be the key to creating cyborg interfaces? Circuitry that ... device, such as a robotic limb or artificial eye might ... components. Writing in the International Journal of Medical ... a "memristor" can be made using human blood. Memristors were ...
Cached Biology News:Fueling interventional radiology growth: SIR Foundation announces Navilyst Medical Pledge 2KIT presents innovations at the 2011 Hannover Messe 2KIT presents innovations at the 2011 Hannover Messe 3KIT presents innovations at the 2011 Hannover Messe 4KIT presents innovations at the 2011 Hannover Messe 5Blood simple circuitry for cyborgs 2
Borosilicate Glass. Certified for use with the Beacon 2000 Systems, 1,000 tubes/case...
... 2000 One-Step FP Standardization Kit is ... low fluorescence polarization values of fluorescein ... Beacon 2000 System. It also allows ... generated by their instrument to the ...
Whole blood is collected from mice (Swiss Webster, 8-10 weeks old,either sex) and processed within 45 minutes of collection. The complement is then filtered and pooled on ice, bottled and stored at -...
Whole blood is collected from healthy guinea pigs of either sex from US source which are free of visible diseases at time of blood collection. Hemolytic titer: CH50 = 435 units...
Biology Products: