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Oil-Free PCR Using the Hot Top Assembly

oCycler family feature a unique four-block design and a robotic arm that moves a paddle containing PCR tubes from block to block at user-specified times. The standard paddle of the main unit of the RoboCycler temperature cycler can be replaced by the Hot Top Assembly, which is an independently powered unit.

RoboCycler units fitted with Hot Top Assemblies heat reaction tubes from the top and sides as well as the bottom. This uniform heating dramatically reduces the amount of condensation that forms during cycling and eliminates the need for oil or wax overlays on PCR reactions. Because the Hot Top Assembly features an automatic shut-off function and over-temperature sensor, PCR amplifications can be run safely overnight. Individual at-temperature and power-on indicator lights provide a quick visual appraisal of the instruments status. The Hot Top Assembly is now available either as an individual unit that can be added to any existing RoboCycler units or as an option on newly purchased RoboCycler 40 or 96 temperature cyclers.1

Each purchase of a Hot Top Assembly for the RoboCycler 96 temperature cycler includes an upgraded version of the software that controls the main unit. The software PC card containing the updated software is simply inserted into the PC card slot located on the right side of the RoboCycler main unit, adapting any RoboCycler 96 temperature cycler for use with the Hot Top Assembly. The updated version of the software comes installed on new RoboCycler 96 temperature cyclers purchased with the Hot Top Assembly option. The upgraded version of the software provides more precise temperature control when using the Hot Top Assembly and, in the gradient model, maintains a linear, 11C temperature gradient across the annealing block, from a minimum temperature of 45C up to a maximum temperature of 99C. In single-temperature mode, the annealing block maintains a stable temperature bet
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