Navigation Links
Oil-Free PCR Using the Hot Top Assembly

Hot Top Assembly now available for Robocycler 96 temperature cyclers

Michelle Cayouette Jane Moores Michael Steege Jason Capodanno Michael Rahilly
Stratagene Cloning Systems, Inc.

With the introduction of the Hot Top Assembly for RoboCycler 96 temperature cyclers, researchers can begin running oil-free PCR amplifications. Each Hot Top Assembly for RoboCycler 96 cyclers is supplied with a software upgrade card, which provides more accurate temperature control. Hot Top Assemblies are available as options on newly purchased RoboCycler temperature cyclers.# Individual units that can be added to existing RoboCycler 40 or 96 units are also available. Using both gradient and single-temperature RoboCycler 96 models, we show the yield and specificity of PCR products to be equivalent before and after the addition of the Hot Top Assembly and software upgrade. Furthermore, PCR results at a given annealing temperature were reproducible whether the annealing block was run in gradient or single-temperature mode, an important feature for optimizing annealing temperatures. When used for small-volume cycle sequencing experiments, the Hot Top Assembly did not affect results adversely, and gel loading was quicker and easier without oil or wax overlays on reactions.

Stratagenes line of RoboCycler temperature cycler currently includes four models. The RoboCycler 40 temperature cyclers accommodate up to forty 600-l individual PCR tubes; the RoboCycler 96 units are high-throughput models that accommodate either a 96-well plate or ninety-six 200-l individual or strip tubes. Both the RoboCycler 40 and 96 temperature cyclers are available in either gradient or nongradient models. Gradient models facilitate PCR optimization by allowing researchers to simultaneously test multiple annealing temperatures. All members of the Rob
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
2. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
3. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
4. Using XL1-Red Mutator Strain to Generate Esterase Variants
5. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
6. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
7. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
8. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
9. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
10. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
11. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Oil Free PCR Using the Hot Top Assembly

(Date:9/19/2014)... 2014  Nektar Therapeutics (NASDAQ: NKTR ... the analgesic profiles of a series of the ... molecules. The preclinical research candidates were created using ... The analgesic properties of kappa receptor agonism ... Kappa opioid receptors are expressed in the ...
(Date:9/19/2014)... Florida , September 19, 2014 ... release latest developments, patents and results of studies and ... Eli Lilly and Company (NYSE: LLY ), ... Corporation (NASDAQ: CELG ) and Arena Pharmaceuticals ... a leader in developing prodrug therapeutics for the treatment ...
(Date:9/19/2014)... Minnesota (PRWEB) September 19, 2014 ... related food industries will gather October 5–8, 2014, ... the Rhode Island Convention Center in Providence, Rhode ... feature the work of food scientists, chemists, microbiologists, ... control, and food production management. To date, 1,000 ...
(Date:9/19/2014)... Massachusetts , September 19, 2014 ... the 53rd European Society for Paediatric Endocrinology (ESPE) Meeting  ... of science and medical research in the field of ... Merck KGaA, Darmstadt, Germany , today ... Innovation (GGI) for 2014. The awards were announced during ...
Breaking Biology Technology:Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 2Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 3Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 4Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 5Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 2Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 3Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 4Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 5Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 6Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 7Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 2Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 3EMD Serono Awards Grant for Growth Innovation (GGI) for the First Time 2EMD Serono Awards Grant for Growth Innovation (GGI) for the First Time 3
... , RESEARCH TRIANGLE PARK, N.C. , Feb. 4 ... company developing orally available antiviral therapeutics, announced today that ... as Chief Medical Officer.   , Dr. Painter has more ... work in clinical development, clinical safety and pharmacovigilance, and occupational ...
... ... at India World CEO Forum , ... New Delhi (Vocus) -- Industry has a unique opportunity today to engage, innovate and ... P. Vergnano at the World CEO Forum of the Delhi Sustainable Development Summit ...
... RICHMOND, Calif. , Feb. 3 Sangamo BioSciences, Inc. (Nasdaq: ... and accomplishments and provided an outlook for 2010. , For the fourth ... of $2.4 million , or $0.05 per share, compared to ... share, for the same period in 2008. As of December 31, 2009 ...
Cached Biology Technology:Chimerix Names Wendy Painter, M.D., M.P.H., as Chief Medical Officer 2Chimerix Names Wendy Painter, M.D., M.P.H., as Chief Medical Officer 3Sustainability Is A Business Imperative 2Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 2Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 3Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 4Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 5Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 6Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 7Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 8Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 9Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 10Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 11Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 12Sangamo BioSciences Reports Fourth Quarter and Full Year 2009 Financial Results 13
(Date:9/19/2014)... Nxt-ID, Inc. (Nasdaq: NXTD and NXTDW) ... growing m-commerce market, updates the "Wocket in Your Pocket" celebrity ad ... light middleweight weight classes, Vinny Pazienza (PAZ). ... Game of Thrones actor Ciaran Hinds and ... Katey Sagal have been cast in Bleed for ...
(Date:9/18/2014)... moon of Uranus, is one of the most visually ... its relatively small size, Miranda appears to have experienced ... formation of at least three remarkable and unique surface ... coronae are visible in Miranda,s southern hemisphere, and each ... the largest, has ridges and troughs with up to ...
(Date:9/18/2014)... matter how many times it,s demonstrated, it,s still ... , But by using a signaling system ... their behavior to suit their population. In short, ... present, and act accordingly. , Once the population ... change from innocuous to pathogenic, or from unhelpful ...
Breaking Biology News(10 mins):NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 2NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 3Miranda: An icy moon deformed by tidal heating 2Miranda: An icy moon deformed by tidal heating 3Miranda: An icy moon deformed by tidal heating 4Miranda: An icy moon deformed by tidal heating 5Miranda: An icy moon deformed by tidal heating 6Miranda: An icy moon deformed by tidal heating 7Researchers study vital 'on/off switches' that control when bacteria turn deadly 2Researchers study vital 'on/off switches' that control when bacteria turn deadly 3
... the scientific journal Zootaxa , researchers from Madagascar and ... The new bird was named Mentocrex beankaensis , ... and the new species beankaensis being coined after ... This species was distinguished from another in the same genus, ...
... by Edward Yu of Iowa State University and the Ames Laboratory ... pumps toxins from bacteria and allows them to resist antibiotics. ... journal Nature . The paper describes the co-crystal structure ... removes heavy metal toxins from bacteria. A research team led by ...
... compounds during the last year in search of potential new ... that one substance shows unusual promise. The early positive signs ... and cell cultures, they say in a report in ACS, ... colleagues, who include Nobel Laureate Stanley B. Prusiner, explain that ...
Cached Biology News:New bird to science emphasizes the critical need to conserve the remaining dry forests of Madagascar 2Iowa State, Ames Lab researchers describe the pump that bacteria use to resist drugs 2
Human Norrin Biotinylated Affinity Purified PAb Protein Family: Other Growth Factors, Other Wnt-related Molecules...
Human VASA Biotinylated Affinity Purified PAb...
Immunogen: Synthetic Peptide: C N(185) D G T E F G G S I Y Q K(197) Storage: -20 C, Avoid Freeze/Thaw Cycles...
interferon-related developmental regulator 2...
Biology Products: