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Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

amounts of DNA template is demonstrated in figure 3. Similar amounts of a 1.9-kb PCR product were amplified from genomic DNA after 30 cycles with Pfu DNA polymerase and after 24 cycles with PfuTurbo DNA polymerase. When the DNA template concentration was varied, PfuTurbo DNA polymerase generated the same amount of PCR product as Pfu DNA polymerase using approximately 75% less genomic DNA. These results suggest that PfuTurbo DNA polymerase is useful for both high-fidelity and higher-throughput PCR applications.

figure 3.

PCR Applications of PfuTurbo DNA Polymerase

The features of Stratagenes PCR enzymes are summarized (table 1). As described in this article, PfuTurbo DNA polymerase is a robust PCR enzyme that amplifies genomic DNA targets up to 10 kb in length and cloned targets up to 15 kb in length with the highest replication fidelity possible. In general, PfuTurbo DNA polymerase amplifies complex targets in higher yield than Taq DNA polymerase or other commercially available proofreading PCR enzymes. Moreover, high-fidelity amplification of vector targets up to 10 kb in length and genomic targets up to 6 kb in length can be achieved using 1-minute-per-kb extension times rather than the 2-minute-per-kb extension times suggested for Pfu DNA polymerase.2

Stratagene recommends the TaqPlus Precision or TaqPlus Long PCR systems when amplifying genomic DNA targets greater than 6 kb with 1-minute-per-kb extension times. The TaqPlus Precision PCR system exhibits an error rate that is significantly lower than that of Taq DNA polymerase and is the enzyme mixture of choice when replication fidelity is the primary consideration. The TaqPlus Long PCR System, on
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