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Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

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TaqPlus Long System

7.6 1.2

0.5-1

0-35

0-18.5

Taq DNA Polymerase

8.0 3.9

1

0-10

0-5

*Error rate equals mutation frequency per base pair per duplication.1

PfuTurbo DNA Polymerase for Higher-Throughput PCR Applications

In addition to achieving higher PCR product yields, PfuTurbo DNA polymerase allows the use of shorter extension times, reduced cycle numbers or lower DNA template concentrations than are required in standard PCR protocols based on Pfu DNA polymerase. The data in Figure 1 and figure 2 demonstrate that PfuTurbo DNA polymerase synthesizes moderate-to-high yields of PCR product using extension times of 1 minute per kb of target, as compared to 2 minutes per kb of target, which is usually required for Pfu DNA polymerase.2 The utility of PfuTurbo DNA polymerase in PCR applications requiring fewer cycle numbers or lower
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