Navigation Links
Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

ymerase was shown to successfully amplify vector targets up to 10 kb using 1-minute-per-kb extension times (lanes 13, 15 and 17) and a 14-kb lambda DNA target using 2-minute-per-kb extension times (lane 19). When relatively short vector targets were amplified, the most dramatic differences in PCR product yield between PfuTurbo and Pfu DNA polymerases were observed when limiting amounts of DNA template were used. For example, PfuTurbo DNA polymerase produced significantly more 1.9-kb PCR product when amplification was performed for 30 cycles using 1 pg of plasmid DNA (lane 13). PfuTurbo and Pfu DNA polymerases generated comparable yields of product when 10 pg of plasmid DNA was used (data not shown). Dramatic differences in yield between PfuTurbo and Pfu DNA polymerases were also observed when amplifying longer vector targets. For example, Pfu DNA polymerase amplified an 8-kb target from lambda phage DNA (lane 16), but not a 14-kb lambda DNA target (lane 20) nor a 10-kb, 40-copy amplicon from transgenic mouse genomic DNA (lane 18). In contrast, PfuTurbo DNA polymerase synthesized high yields of all of the longer vector targets (lanes 15, 17 and 19).

In total, the data demonstrate that PCR product yields generated with PfuTurbo DNA polymerase are higher than those generated with Pfu DNA polymerase (figure 2) or with other single PCR enzymes (Figure 1). PfuTurbo DNA polymerase can be used routinely in all PCR applications that utilize Taq DNA polymerase or a proof- reading PCR enzyme (e.g., Pfu, VentR, Deep VentR, Pwo, KOD and Ultma DNA polymerases). PfuTurbo DNA polymerase is particularly suited for amplifying longer (>2 kb) or complex genomic DNA targets, which are not readily amplified by single D
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11

Related biology technology :

1. High-Fidelity PCR with a Novel Polymerase Mixture
2. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
5. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
6. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
7. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
10. Enhancing PCR* Efficiency with MasterTaq
11. Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology
Post Your Comments:
(Date:8/31/2015)... SAN DIEGO , Aug. 31, 2015 ... drug development company announces that the Company,s lead antibody, ... subject of five separate presentations during the upcoming World ... Hawaii September 2-5, 2015. Researchers from the ... (MSK) will present results on the use of MabVax,s ...
(Date:8/31/2015)... Research and Markets ( http://www.researchandmarkets.com/research/cbgl72/middle_east_and ) has ... Africa Biomedical Sensors Market - Growth, Trends & Forecasts ... The Middle East & Africa ... 2018 at a CAGR of 3.26% over the period ... sensors that are adaptable to the genetic formulation of ...
(Date:8/31/2015)... 31, 2015  US-Australian drug discovery company, Novogen ... confirmed its comprehensive scientific review has identified high ... areas of unmet patient need. Novogen is moving ... projects prior to entering into Phase 1 clinical ... Acting Chief Executive Officer, Iain Ross , ...
(Date:8/31/2015)... Aug. 31, 2015 Trimb Healthcare AB ("Trimb") ... acquire YouMedical Group BV ("YouMedical"), a Dutch OTC company ... annual sales of approximately EUR 16 million, with solid ... "Our ambition is to build a leading international ... toward that goal. YouMedical gives us a stronger North ...
Breaking Biology Technology:MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 2MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 3MabVax Therapeutics HuMab 5B1 Antibody Is Highlighted at Upcoming World Molecular Imaging Congress in Five Separate Presentations 4Middle East & Africa Biomedical Sensors Market Report 2015 - Growth, Trends & Forecasts to 2020 2Novogen Announces The Outcome Of A Comprehensive Science Review 2Novogen Announces The Outcome Of A Comprehensive Science Review 3Novogen Announces The Outcome Of A Comprehensive Science Review 4Novogen Announces The Outcome Of A Comprehensive Science Review 5Novogen Announces The Outcome Of A Comprehensive Science Review 6
... now has the most,consumer-friendly healthcare system in Europe, ... The EHCI compares all the EU 27 national,healthcare ... point of view, measured by 27 indicators across ... 2006 winner,France in third place. Closely behind the ...
... Abiant, Inc., an Illinois based company,specializing advanced ... disease diagnosis, announces the successful closing of ... an angel,network specializing in early-stage technology, syndicated ... participants. Abiant has developed,partnerships with large pharmaceutical ...
... A novel device, developed by a team led ... traps, detects and manipulates the single spin of ... prevented progress toward spintronics and spin-based quantum computing., ... the research paper brings closer to reality electronic ...
Cached Biology Technology:European Union has 'Three-Speed' Healthcare System - Many Countries Lagging in Reform 2Abiant, Inc. Announces Close on Investment Round 2Quantum device traps, detects and manipulates the spin of single electrons 2
(Date:8/12/2015)...  New research unveiled at Black Hat 2015 ... steal users, fingerprints. Information released today by HYPR ... on mobile devices, making the security of this ... mobile devices, HYPR Corp. recently released a biometric ... with strong cryptographic security. More information on this ...
(Date:8/10/2015)... 10, 2015 The latest 364 page ... analysis of the global border security market . ... $16.4bn in 2015. Now: Border security is hard ... of the business critical issue you need to know about ... ,s objective analysis of how this will impact your company ...
(Date:8/5/2015)... According to a new market research report, ... (BFSI, Airport, IT & Telecom, Utilities, Education, Defense & ... published by MarketsandMarkets, defines and segments the Physical Identity ... global PIAM Market is estimated to grow from $272.2 ... a CAGR of 14.9 % from 2014 to 2019. ...
Breaking Biology News(10 mins):HYPR Corp. Addresses Android Vulnerability That Exposes Ecosystem-wide Biometric Security Challenges 2HYPR Corp. Addresses Android Vulnerability That Exposes Ecosystem-wide Biometric Security Challenges 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 2Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 4Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 5Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 6Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 7Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 8Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 9Physical Identity and Access Management Market Worth $546.2 Million by 2019 2Physical Identity and Access Management Market Worth $546.2 Million by 2019 3Physical Identity and Access Management Market Worth $546.2 Million by 2019 4
... tidal energy sufficient to power about half of Scotland could ... north coast of the country, engineers say. Researchers ... tidal power could be generated by turbines placed in the ... gigawatts (GW) could be available. The in-depth assessment by ...
... unusually strong El Ninos, such as the 1983 heatwave ... are likely to double in number as our planet ... the ARC Centre of Excellence for Climate System Science ... CSIRO, published their findings in the journal Nature ...
... in parts of Africa, Asia and South America contributes to ... world,s poor. The parasitic worm lives in the soil and ... blood, the worms cause anemia and, in children, stunted growth ... of Medicine in St. Louis have decoded the genome of ...
Cached Biology News:Island channel could power about half of Scotland, studies show 2Get used to heat waves: Extreme El Nino events to double 2Decoded: DNA of blood-sucking worm that infects world's poor 2
... DNA Visualizer Extraction Kit is manufactured for ... DNA damage byexposure to UV light. The ... is often used for DNA-cloning work. The ... bromide and exposure of UV light damages ...
CleanBox DNA workstation with two 254nm UV lambs & 12-hr timer, 90cm (36-inch)width, single door, 115V (w/crating)...
Mouse monoclonal antibody raised against a full length recombinant PPIL2. NCBI Entrez Gene ID = PPIL2...
Mouse monoclonal antibody raised against a partial recombinant ALAS2. NCBI Entrez Gene ID = ALAS2...
Biology Products: