( DNA polymerase hasbeen shown to be i...)Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

DNA polymerase has been shown to be ideally suited for both high-fidelity and higher-throughput PCR applications. In this article, we demonstrate that PCR product yields and replication fidelity are significantly higher with PfuTurbo DNA polymerase than with other PCR enzymes.

PCR Performance of PfuTurbo DNA Polymerase

Figure 1 shows the results of amplifying 0.9-kb and 5.2-kb targets from human genomic DNA using PfuTurbo DNA polymerase and several other PCR enzymes. For these PCR amplifications, extension times of 1 minute per kb of target and varying amounts of each polymerase (from 0.5 to 5 U) were used. All of the enzymes tested successfully amplified the 0.9-kb target. The reaction products shown in lanes 1-7 were generated using 2 to 2.5 U of each DNA polymerase, with the exception that KOD DNA polymerase required 1.25 U to obtain sufficient yields with minimal smearing. Higher yields of PCR products were obtained when 5 U of Pfu, Pwo or Taq DNA polymerase was used (data not shown). Smearing or reduced yield was observed when higher amounts of KOD, Vent_R or Deep Vent_R DNA polymerases were used (data not shown).

The 5.2-kb target was also amplified with varying amounts (from 0.5 to 5 U) of each DNA polymerase. The reactions shown in Figure 1 (lanes 8-14) are those that exhibited the highest yields of PCR products (for PfuTurbo and Taq DNA polymerases) or those that were carried out using the highest possible number of polymerase units before excessive smearing was observed. Only PfuTurbo (lane 8) and Taq (lane 14) DNA polymerases successfully amplified the 5.2-kb target, and
'"/>

Source:

Page: All 1 2 3 4 5 6 7 8 9 10 11
Related biology technology :

1. High-Fidelity PCR with a Novel Polymerase Mixture
2. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
5. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
6. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
7. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
10. Enhancing PCR* Efficiency with MasterTaq
11. Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology