Navigation Links
Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

fers.3 In general, however, PCR product yields decrease as the length of an amplicon increases, irrespective of the PCR enzyme used.

The recent development of DNA polymerase mixtures has largely overcome the problem of reduced yields when amplifying longer targets (e.g., >4-kb genomic targets) with single DNA polymerases. For example, Stratagene has developed two DNA polymerase mixtures containing Taq and Pfu DNA polymerases: TaqPlus Long PCR system**,, for amplifying targets up to 35 kb in length4 and TaqPlus Precision PCR system**, for high-fidelity amplification of genomic targets up to 10 kb and cloned targets up to 15 kb.5 DNA polymerase mixtures typically consist of a blend of a nonproofreading DNA polymerase (e.g., Taq DNA polymerase) and a proofreading DNA polymerase (e.g., Pfu DNA polymerase). The addition of the proofreading enzyme is thought to enhance amplification of longer targets by repairing mispaired primer termini that Taq DNA polymerase cannot extend.6 However, because DNA polymerase mixtures are composed of a significant proportion of a nonproofreading DNA polymerase, error rates for mixtures are closer to that of Taq DNA polymerase and approximately 3- to 6-fold higher than the error rate of Pfu DNA polymerase.1,4,5

In a continuing effort to improve high-fidelity PCR performance, Stratagene has discovered a novel thermo- stable factor that enhances the performance of Pfu DNA polymerase in PCR amplifications without altering the enzymes high replication fidelity. This PCR enhancing factor was isolated from Pyrococcus and shown to lack both DNA polymerase and exonuclease activities (data not shown). PfuTurbo DNA polymerase is an optimized blend of cloned Pfu DNA polymerase and this PCR enhancing factor. PfuTurbo
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11

Related biology technology :

1. High-Fidelity PCR with a Novel Polymerase Mixture
2. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
3. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
4. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
5. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
6. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
7. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
10. Enhancing PCR* Efficiency with MasterTaq
11. Enhancing Ruggedness and Full-Scan MS Sensitivity Using Ion Sweep Technology
Post Your Comments:
(Date:12/24/2014)... December 23, 2014 On Friday, ... H.R. 83, the Omnibus and Continuing Resolution Appropriations ... made hydrocephalus a condition eligible to receive funding ... administered by the Department of Defense (DoD). The ... allies on Capitol Hill, is celebrating this victory ...
(Date:12/24/2014)... Nashville Fertility Center announced that Dr. ... American Society for Reproductive Medicine (ASRM), a leading professional ... medicine. In his new role as treasurer, Dr. ... and will have a voice in furthering the mission ... ASRM since 1984 when he joined while conducting fellowship ...
(Date:12/24/2014)... 23, 2014 China Biologic Products, Inc. ... leading fully integrated plasma-based biopharmaceutical company in ... majority-owned subsidiary, Shandong Taibang Biological Products Co. Ltd., ... the China Food and Drug Administration (the "CFDA") ... previously disclosed in the Company,s public filings, the ...
(Date:12/24/2014)... LOUIS , Dec. 23, 2014 ... today that the waiting period under the Hart-Scott-Rodino ... the acquisition expired on December 22, 2014, thereby ... review requirement for the acquisition of the Company ... U.S. antitrust clearance satisfies another condition to closing ...
Breaking Biology Technology:Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 2Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 3China Biologic Receives GMP Certification for New Coagulation Factor Facility 2China Biologic Receives GMP Certification for New Coagulation Factor Facility 3Acquisition of Sigma-Aldrich by Merck KGaA, Darmstadt, Germany, Receives U.S. Antitrust Clearance 2Acquisition of Sigma-Aldrich by Merck KGaA, Darmstadt, Germany, Receives U.S. Antitrust Clearance 3
... Everyone wants to design the dsRNA that , ... length correlate with silencing efficiency? Ambion scientists , ... dsRNA of different sizes and directed , ... (Figure 1A), were prepared using the MEGAscript RNAi Kit ...
... Large scale gene expression studies , ... purify high quality RNA from a large number of samples. Although the , ... dsRNA production and , clean up, ... format. Thus for high-throughput applications, we recommend , ...
... , ... heterogenous samples is a major challenge in studying gene , ... microdissection (LCM), developed in a collaborative ... and Arcturus, Inc. specifically to isolate , ...
Cached Biology Technology:RNAi: Size Does Matter 2RNAi for High Throughput Analysis 2RNA from LCM Samples 2RNA from LCM Samples 3
(Date:11/21/2014)... REDMOND, Wash. , Nov. 20, 2014 ... access for the Internet of Things (IoT), today announced ... position of chief operating officer. Previously a strategic advisor ... marketing, finance, and operations. Mr. Traynor is based out ... . He reports to Chris Muench , ...
(Date:11/18/2014)... WASHINGTON , Nov. 18, 2014  The ... Security Industry Association today jointly announce the formation ... and release of its Identity and Biometric ... (Framework). The Framework received official support from BORDERPOL, ... governments to improve and provide expertise regarding border ...
(Date:11/18/2014)... 2014 The Parenteral Drug Association (PDA) today confirmed ... speak and at least seven more will participate in the ... Shoreham Hotel in Washington D.C. , Dec. ... significant support from the regulatory agencies in the ... in our effort to help advance the use of metrics ...
Breaking Biology News(10 mins):C-Labs Names Former Microsoft and Bsquare Executive as Chief Operating Officer 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2
... percent of mares have difficulties conceiving. The most common cause ... the mucusal membrane of the uterus caused by the bacteria ... noticed that many horses that test negative for streptococci have ... of symptoms, however, it still seems to reduce the fertility ...
... richer the assortment of amphibian species living in a pond, ... has against a parasitic infection that can cause severe deformities, ... study by the University of Colorado Boulder. The ... support the idea that greater biodiversity in larger-scale ecosystems, such ...
... NY. (Feb. 13, 2013) Researchers at the Johns ... Research Centre in Warsaw, Poland, have found that nonself-donated ... immunocompetent research mice when the grafts are injected into ... into the forceps minor (FM) region. In their ...
Cached Biology News:Researchers make racehorses fertile 2CU-Boulder amphibian study shows how biodiversity can protect against disease 2CU-Boulder amphibian study shows how biodiversity can protect against disease 3CU-Boulder amphibian study shows how biodiversity can protect against disease 4Impact of stem cell transplantation location in brain a crucial factor for cell survival 2
MAb to HLA, Class II-DR+DP Dry Ice: No Preservative: NaN3 Availability When Not in Inventory: 14-21 Business Days...
... surface is produced by a patented microwave ... giving more consistent, even cell attachment, increased ... attachment and growth uunder difficult conditions, including ... may provide a more ecominic alternative to ...
... array system, 100-240 V, uses multiplex suspension ... analysis of up to 100 proteins and ... suspension array is composed of up to ... may be conjugated with a unique reactant ...
... PYREX Delong style shaker flasks have three ... agitation of solutions to improve oxygen or ... reciprocating shakers. The long neck reduces splashing ... Their uniform wall thickness provides the proper ...
Biology Products: