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Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase

e 3, the temperature cycling parameters for the 1.9-kb target were as follows: 1 cycle at 95C for 1 minute; 20-30 cycles at 95C for 1 minute (denaturation), 50C for 1 minute (annealing) and 72C for 1 minute and 54 seconds (extension) and a final extension cycle of 72C for 10 minutes.

REFERENCES

  1. Cline, J., Braman, J.C., and Hogrefe, H.H. (1996) Nucleic Acids Res. 24: 3546-3551.

  2. Nielson, K.B., Costa, G.L., and Braman, J. (1996) Strategies 9: 24-25.

  3. Nielson, K.B., Braman, J., and Kretz, K. (1995) Strategies 8: 26.

  4. Nielson, K.B., et al. (1994) Strategies 7: 64.

  5. Nielson, K.B., Cline, J., Bai, F., McMullan, D., McGowen, B., and Hogrefe, H. (1997) Strategies 10: 29-32.

  6. Barnes, W.M. (1994) Proc. Natl. Acad. Sci. USA 91: 2216-2220.

  7. Guide to Pfu DNA Polymerase. (1996) Stratagene, La Jolla, California.

* Patent pending
** US Patent No. 5,556,772 and patents pending


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