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Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase


pfuturbo DNA polymerase for robust, high-fidelity PCR amplification

Holly Hogrefe Brad Scott Kirk Nielson Valerie Hedden Connie Hansen Janice Cline Frances Bai Jeff Amberg Ronda Allen Mark Madden
Stratagene

Stratagene announces the release of pfuturbo DNA polymerase,*, an improved version of Pfu DNA polymerase for high-fidelity PCR. PfuTurbo DNA polymerase is a blend of cloned Pfu DNA polymerase and a novel thermostable factor that enhances PCR product yields without altering the fidelity of DNA replication. PfuTurbo DNA polymerase can be used to amplify complex genomic DNA targets up to 10 kb in length and vector targets up to 15 kb in length. PfuTurbo DNA polymerase amplifies complex targets in higher yield than Taq DNA polymerase or other commercially available proofreading PCR enzymes. The error rate of PfuTurbo DNA polymerase is equal to that of Pfu DNA polymerase and is significantly lower than the error rates of other proofreading enzymes, DNA polymerase mixtures and Taq DNA polymerase. The enhanced performance of PfuTurbo DNA polymerase allows the use of shorter extension times, fewer PCR cycles and lower concentrations of DNA templates than are required for Pfu DNA polymerase. These attributes make PfuTurbo DNA polymerase ideally suited for both high-fidelity and higher-throughput PCR applications.

Pfu DNA polymerase exhibits the lowest error rate of any thermostable DNA polymerase characterized to date,1 making it the enzyme of choice for high-fidelity PCR applications. Pfu DNA polymerase can amplify high yields of genomic DNA targets up to 4 kb in length using PCR extension times of 2 minute per kb of target.2 Longer genomic targets may be successfully amplified with Pfu DNA polymerase using higher amounts of polymerase, longer extension times or modified PCR reaction buf
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