miRNA Array Technology
Enables sensitive miRNA expression
profiling between samples--providing critical information
about gene expression regulation
Involves a comprehensive probe set corresponding to all known human and mouse miRNAs--allowing thorough analysis
Detects as little as 0.1 fmol miRNA--allowing analysis of low amounts of starting material
The miRNA array protocol (Figure 1), which is composed of the mirVana miRNA Labeling Kit and mirVana miRNA Probe Set, enables sensitive analyses of miRNA expression profiles. This miRNA expression profiling system can identify miRNAs that are differentially expressed across tissues and cell lines, as well as those that are differentially expressed in virtually any biological sample.
Figure 1. miRNA Array Procedure. (A) miRNA are purified from tissue or cell samples (e.g. using Ambion's mirVana miRNA Isolation Kit and an electrophoresis unit specialized for small RNA purification). Amine NTPs are incorporated during tailing with poly(A) polymerase. miRNA are then labeled with amine reactive dyes. After hybridization with probes on a microarray slide, dye signals are analyzed. (B) Representative hybridization signals from an miRNA array procedure. Red: Cy3; Green: Cy5.
mirVana miRNA Labeling Kit
* Novel technology efficiently labels all miRNAs in the sample
* Highly labeled miRNAs suitable for array analysis
* Simple procedure is compatible with any amine reactive dye, including Cy3 and Cy5
* Positive control target matches control probe in the mirVana miRNA Probe Set
mirVana miRNA Probe Set
* Large probe set is specific for nearly 200 unique human and mouse miRNAs
* Each probe has been functionally verified
* The simple printing process is compatible with both epoxy and aldehyde slides
* Data normalization is easy with provided 4 negative controls and one positive control.
To see miRNA expression profiling data generated using this new system, see MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers. For more information, or to view the entire miRNA array protocol, visit the miRNA Online Resource at www.ambion.com/miRNA.