Navigation Links
Novel MicroRNA Array Technology for Sensitive miRNA Profiling

Uncover Critical Regulatory Pathways by Analyzing a Complete Set of Human and Mouse miRNAs


The miRNA gene family contains hundreds of translation regulators, yet their presence and function has only recently been reported in the literature (see MicroRNAs: Isolation to Functional Analyses and MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers for a detailed overview of the miRNA field). The miRNA array protocol described here is the first commercial solution for sensitive miRNA expression profiling.


miRNA Array Technology

Enables sensitive miRNA expression profiling between samples--providing critical information about gene expression regulation
Involves a comprehensive probe set corresponding to all known human and mouse miRNAs--allowing thorough analysis
Detects as little as 0.1 fmol miRNA--allowing analysis of low amounts of starting material

The miRNA array protocol (Figure 1), which is composed of the mirVana miRNA Labeling Kit and mirVana miRNA Probe Set, enables sensitive analyses of miRNA expression profiles. This miRNA expression profiling system can identify miRNAs that are differentially expressed across tissues and cell lines, as well as those that are differentially expressed in virtually any biological sample.

Figure 1. miRNA Array Procedure. (A) miRNA are purified from tissue or cell samples (e.g. using Ambion's mirVana miRNA Isolation Kit and an electrophoresis unit specialized for small RNA purification). Amine NTPs are incorporated during tailing with poly(A) polymerase. miRNA are then labeled with amine reactive dyes. After hybridization with probes on a microarray slide, dye signals are analyzed. (B) Representative hybridization signals from an miRNA array procedure. Red: Cy3; Green: Cy5.


mirVana miRNA Labeling Kit

* Novel technology efficiently labels all miRNAs in the sample

* Highly labeled miRNAs suitable for array analysis

* Simple procedure is compatible with any amine reactive dye, including Cy3 and Cy5

* Positive control target matches control probe in the mirVana miRNA Probe Set


mirVana miRNA Probe Set

* Large probe set is specific for nearly 200 unique human and mouse miRNAs

* Each probe has been functionally verified

* The simple printing process is compatible with both epoxy and aldehyde slides

* Data normalization is easy with provided 4 negative controls and one positive control.

To see miRNA expression profiling data generated using this new system, see MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers. For more information, or to view the entire miRNA array protocol, visit the miRNA Online Resource at www.ambion.com/miRNA.


back to top


Ordering Information Cat# Product Name Size 1562 mirVana miRNA Labeling Kit 20 rxns 1564V1 mirVana miRNA Probe Set 1 set
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. High-Fidelity PCR with a Novel Polymerase Mixture
3. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
6. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
7. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
8. Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns
9. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
10. MicroRNA Profiling by Array Analysis Reveals Critical BioMarkers
11. Getting Started with MicroRNA Research
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/11/2019)... Md. and ROANOKE, Va. (PRWEB) , ... June ... ... care optimized for microlearning engagement, and Carilion Clinic, a national leader in opioid ... the latest in virtual care. , Mytonomy recently deployed its virtual ...
(Date:5/31/2019)... ... May 29, 2019 , ... ... the USPTO providing proprietary interest to our methodology, processes, and diagnostic techniques. ... AND TREATMENT… extends Somnology’s IP rights including our proprietary sleep scoring methodology. ...
(Date:5/21/2019)... Ethiopia (PRWEB) , ... May 21, 2019 , ... ... Brauerei in Berlin (VLB) today officially opened the inaugural Africa Brewing Conference dedicated ... and malting industry in Africa. The event, which is supported through a partnership ...
(Date:5/8/2019)... ... ... 16.2 hands, Atlas is on the large side for a Quarter Horse. He and ... activities. When loping around a corner in late 2017, Atlas tripped and partially tore ... being able to walk. Fortunately Atlas’ owner, Kelly, sought the opinion of her veterinarian, ...
Breaking Biology Technology:
(Date:6/19/2019)... ... June 19, 2019 , ... Dahryn Trivedi, today released research results on ... antibiotic which can prove to be beneficial for treating multiple bacterial diseases. , The ... , Over 68% decrease in weight , Over 8% ...
(Date:6/16/2019)... ... June 14, 2019 , ... Emtec, ... will deliver a joint presentation with Versiti’s CIO at the Healthcare Industry ... Rosen Shingle Creek Resort. , Versiti Vice President and Chief Information Officer, Lynne ...
(Date:6/13/2019)... ... June 13, 2019 , ... ... organization exclusively serving the end-to-end clinical and ancillary supply chain management needs of ... and medical research organizations, today announced it will sponsor a summer education program ...
Breaking Biology News(10 mins):