Navigation Links
Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns


Introduction
While full coverage C18 phases have been responsible for the growth of HPLC as a reliable high efficiency technique, it has become apparent in recent years that such column types are not suited for reliable analysis of highly polar compounds under low organic or aqueous mobile phase conditions. It was shown that by incorporating a polar group in the alkyl bonded phase reagent extra retention could be obtained for polar solutes while maintaining the operating characteristics of traditional reversed phase columns. Adding polarity to the bonded alkyl phase also overcame 'bonded phase collapse', the steady decline of retention times under (near) aqueous mobile phase conditions. In this note we report on an alternative method to obtain enhanced retention of polar solutes by creating a low density bonded phase that is fully accessible to water molecules.


Results
TSK-GEL ODS-100V columns contain high purity five micron silica particles with 100 angstrom pores and 450m2/g surface area, which are functionalized by an incomplete reaction with a difunctional octadecylsilane reagent (see Figure 1 ). The low density C18 monolayer is then endcapped with two difunctional dialkylsilane reagents to improve the peak shape of basic compounds, while maintaining stable analysis times in low organic or aqueous mobile phases.


To demonstrate the absence of accessible silanol groups, Figure 2 compares retention and peak shape for two tricyclic antidepressant drugs on four commercial water-wettable columns.


The same commercial columns were also evaluated wi
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. High-Fidelity PCR with a Novel Polymerase Mixture
3. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
6. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
7. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
8. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
9. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
10. Enhanced PCR Cloning System
11. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
Post Your Comments:
(Date:7/24/2014)... A novel combination of microscopy and data processing has ... National Laboratory an unprecedented look at the surface of ... properties. , The research team led by ORNL,s ... a perovskite manganite, a complex material that exhibits dramatic ... surface behavior could benefit researchers who are interested in ...
(Date:7/24/2014)... MENLO PARK, Calif. , July 24, 2014 ... biotechnology company in the emerging field of regenerative ... live webcast of a presentation to investors on ... 1:30 p.m. PDT.  The presentation will include an ... pipeline. To access the webcast, visit http://www.ustream.tv/channel/asterias-biotherapeutics ...
(Date:7/24/2014)... research team led by group leader Yung-Eun Sung ... to synthesize sulfur-doped and nitrogen-doped graphenes which can ... batteries and fuel cells. Yung-Eun Sung is both ... Research at Institute for Basic Science* (IBS) and ... This achievement has great significance with regards to ...
(Date:7/24/2014)... synthetic processes prefer to settle into equilibriuma state ... within the realm of non-equilibrium conditions where new ... energy and phases, such as temperature fluctuations, freezing ... to regulate their body temperature, airplanes to fly, ... , But even though these conditions exist ...
Breaking Biology Technology:ORNL study reveals new characteristics of complex oxide surfaces 2Asterias Biotherapeutics Announces Live Investor Webcast 2Asterias Biotherapeutics Announces Live Investor Webcast 3Asterias Biotherapeutics Announces Live Investor Webcast 4New approach to form non-equilibrium structures 2
... Oct. 23 BioWisdom Ltd (BioWisdom),the market ... industry, today announces the release of OmniViz,Visualization ... are increasingly being used in competitive business ... business decisions.,OmniViz Visualization Software can turn the ...
... Israel and JERSEY CITY, New Jersey, October 23, ... in,the development of microRNA-based diagnostics and therapeutics, today,announced ... Annual,Meeting on "Molecular Markers in Cancer". The event ... in Hollywood, Florida., The poster presentation, titled ...
... FRANCISCO, Calif., Oct. 23 Poniard,Pharmaceuticals, Inc. (Nasdaq: ... today announced that it will hold a conference ... provide a corporate update,including an update on the ... candidate., Jerry McMahon, Ph.D., chairman and chief ...
Cached Biology Technology:Rosetta Genomics to Present at the Upcoming EORTC-NCI-ASCO Annual Meeting on "Molecular Markers in Cancer" 2Rosetta Genomics to Present at the Upcoming EORTC-NCI-ASCO Annual Meeting on "Molecular Markers in Cancer" 3Poniard Pharmaceuticals to Host Third Quarter 2008 Financial Results Conference Call on October 30 2
(Date:7/24/2014)... wildlife loss leads to conflict among people around the ... Society (WCS) Health & Ecosystems: Analysis of Linkages (HEAL) ... approach to tackle global biodiversity decline. , The harvest ... world,s people and provides protein for more than a ... no surprise that today,s unprecedented loss of wildlife, is ...
(Date:7/24/2014)... Bushmeat, the use of native animal species for food ... be a significant factor in the decline of many ... new study indicates that more than half of the ... raptors and hornbills., "By surveying not only the meat ... being eaten inside the forest by hunters and brought ...
(Date:7/24/2014)... Angeles, where electric cars ply silent freeways, solar panels ... beneath the earth, from howling winds and from the ... that it is technically and economically feasible to convert ... renewable energy. Published in Energy , the plan ... energy supply in California that could create tens of ...
Breaking Biology News(10 mins):New study draws links between wildlife loss and social conflicts 2Stanford study shows how to power California with wind, water and sun 2Stanford study shows how to power California with wind, water and sun 3Stanford study shows how to power California with wind, water and sun 4
... French . Some of the human X chromosome ... Africa, according to an international team of researchers led by ... of Montreal and the CHU Sainte-Justine Research Center. The research ... and Evolution. "This confirms recent findings suggesting ...
... July 18, 2011 Researchers at UT Southwestern Medical ... involved in the formation of colon cancer, far more ... study, published in the July 2011 Cancer Research (Priority ... cancer treatments targeting multiple genes and pathways simultaneously. Current ...
... YORK, July 16, 2011 Experts from the Center ... Center will present new research at the 2011 Alzheimer,s ... in Paris, France from July 16 21. ... impairment in retired football players, with Stella Karantzoulis, PhD, ...
Cached Biology News:Genetic research confirms that non-Africans are part Neanderthal 2NYU Langone Medical Center's tip sheet to the 2011 Alzheimer's Association International Conference 2NYU Langone Medical Center's tip sheet to the 2011 Alzheimer's Association International Conference 3NYU Langone Medical Center's tip sheet to the 2011 Alzheimer's Association International Conference 4NYU Langone Medical Center's tip sheet to the 2011 Alzheimer's Association International Conference 5NYU Langone Medical Center's tip sheet to the 2011 Alzheimer's Association International Conference 6
For removal of the zona pellucida. Sterile, Mouse Embryo tested...
... The LXQ linear ion trap mass spectrometer ... analytical laboratory., The LXQ linear ion trap ... and proteomic applications, delivering the fast cycle ... linear ion trap at an attractive price. ...
... scientists who do not have access to microarray ... their biological samples tested for the concentration of ... 1-2-3 Chose the analytes you wish ... S&S antibody menu. Send your samples to ...
... Persistent Analysis Environment 2) Dot plot, ... 3) Software Compensation 4) Cell Cycle ... Platform 7) Intelligent batch generation of graphs ... 9) Single drag-and-drop operations to perform complex ...
Biology Products: