Navigation Links
Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns


Introduction
While full coverage C18 phases have been responsible for the growth of HPLC as a reliable high efficiency technique, it has become apparent in recent years that such column types are not suited for reliable analysis of highly polar compounds under low organic or aqueous mobile phase conditions. It was shown that by incorporating a polar group in the alkyl bonded phase reagent extra retention could be obtained for polar solutes while maintaining the operating characteristics of traditional reversed phase columns. Adding polarity to the bonded alkyl phase also overcame 'bonded phase collapse', the steady decline of retention times under (near) aqueous mobile phase conditions. In this note we report on an alternative method to obtain enhanced retention of polar solutes by creating a low density bonded phase that is fully accessible to water molecules.


Results
TSK-GEL ODS-100V columns contain high purity five micron silica particles with 100 angstrom pores and 450m2/g surface area, which are functionalized by an incomplete reaction with a difunctional octadecylsilane reagent (see Figure 1 ). The low density C18 monolayer is then endcapped with two difunctional dialkylsilane reagents to improve the peak shape of basic compounds, while maintaining stable analysis times in low organic or aqueous mobile phases.


To demonstrate the absence of accessible silanol groups, Figure 2 compares retention and peak shape for two tricyclic antidepressant drugs on four commercial water-wettable columns.


The same commercial columns were also evaluated wi th organic acids under low pH conditions in 2% acetonitrile, as is shown in Figure 3 .



Conclusion
The ability to provide symmetrical peak shapes for basic as well as acidic compounds make TSKgel ODS-100V the column of choice for method development and quantitative analysis of small molecular weight compounds.


Ordering Information


More Infomation
TSKgel ODS-100V



'"/>

Source:


Page: All 1 2

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. High-Fidelity PCR with a Novel Polymerase Mixture
3. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
6. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
7. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
8. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
9. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
10. Enhanced PCR Cloning System
11. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:9/20/2017)... ... ... Diversity focused business accelerator, The Refinery , announced today they are hosting ... technology-driven, women-led startups in Boston, MA, New Haven/Hamden, CT, and Newark, NJ. Fueling ... that week – in Boston, it will be part of the City of Boston's ...
(Date:9/20/2017)... ... September 20, 2017 , ... ... examining the effects of exoskeleton-assisted walking on gait parameters and neuromuscular activity ... "Neuromechanical adaptations during a robotic powered exoskeleton assisted walking session" (doi:10.1080.10790268.2017.1314900) was ...
(Date:9/20/2017)... Aurora, Ohio (PRWEB) , ... September 20, 2017 ... ... automation, systems integration, and building management solutions, announced today the opening of an ... customers in Taiwan and the Greater China region, while developing new relationships in ...
(Date:9/19/2017)... ... September 19, 2017 , ... ... group of funded early-stage tech companies. “Grit” author Angela Duckworth and her team ... the ic@3401 community is Cooley, an international law firm with decades of experience ...
Breaking Biology Technology:
(Date:4/11/2017)... , Apr. 11, 2017 Research and ... Market 2017-2021" report to their offering. ... The global eye tracking market to grow at a ... report, Global Eye Tracking Market 2017-2021, has been prepared based on ... covers the market landscape and its growth prospects over the coming ...
(Date:4/5/2017)... YORK , April 5, 2017 Today ... is announcing that the server component of the HYPR ... known for providing the end-to-end security architecture that empowers ... HYPR has already secured over 15 million ... makers including manufacturers of connected home product suites and ...
(Date:3/30/2017)... HONG KONG , March 30, 2017 ... developed a system for three-dimensional (3D) fingerprint identification by adopting ground ... technology into a new realm of speed and accuracy for use ... applications at an affordable cost. ... ...
Breaking Biology News(10 mins):