Navigation Links
Novel Bonding Chemistry Imparts Enhanced Polar Selectivity to TSK-GEL ODS-100V Reversed Phase Columns

While full coverage C18 phases have been responsible for the growth of HPLC as a reliable high efficiency technique, it has become apparent in recent years that such column types are not suited for reliable analysis of highly polar compounds under low organic or aqueous mobile phase conditions. It was shown that by incorporating a polar group in the alkyl bonded phase reagent extra retention could be obtained for polar solutes while maintaining the operating characteristics of traditional reversed phase columns. Adding polarity to the bonded alkyl phase also overcame 'bonded phase collapse', the steady decline of retention times under (near) aqueous mobile phase conditions. In this note we report on an alternative method to obtain enhanced retention of polar solutes by creating a low density bonded phase that is fully accessible to water molecules.

TSK-GEL ODS-100V columns contain high purity five micron silica particles with 100 angstrom pores and 450m2/g surface area, which are functionalized by an incomplete reaction with a difunctional octadecylsilane reagent (see Figure 1 ). The low density C18 monolayer is then endcapped with two difunctional dialkylsilane reagents to improve the peak shape of basic compounds, while maintaining stable analysis times in low organic or aqueous mobile phases.

To demonstrate the absence of accessible silanol groups, Figure 2 compares retention and peak shape for two tricyclic antidepressant drugs on four commercial water-wettable columns.

The same commercial columns were also evaluated wi th organic acids under low pH conditions in 2% acetonitrile, as is shown in Figure 3 .

The ability to provide symmetrical peak shapes for basic as well as acidic compounds make TSKgel ODS-100V the column of choice for method development and quantitative analysis of small molecular weight compounds.

Ordering Information

More Infomation
TSKgel ODS-100V



Page: All 1 2

Related biology technology :

1. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
2. High-Fidelity PCR with a Novel Polymerase Mixture
3. Challenge the Performance of Your Hot-Start PCRs with FastStart Taq DNA Polymerase and the Novel FastStart High Fidelity PCR System
4. Combine High Yield, Great Accuracy and the Prevention of Carry-over Contamination by Using the Novel Expand High FidelityPLUS PCR System
5. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
6. A Novel Process for Gene Expression Profiling of Rat and Mouse Tissues from Formalin-Fixed Paraffin-Embedded Sections Using Microarrays
7. Use of Novel FlashPlate Technology to Measure cAMP Accumulation in Chinese Hamster Ovary Cells Expressing Human -2 Adrenoreceptors
8. ESR1 Gene Expression in Oncology and Metabolic Diseases Using the ASCENTATM System Target Validation and Identification of Novel Disease Indications
9. Unique Surface Treatment of Novel TSK-GEL ODS Columns Provide Separation of Aqueous and Fat Soluble Vitamins
10. Enhanced PCR Cloning System
11. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
Post Your Comments:

(Date:11/24/2015)... 2015 /CNW Telbec/ - ProMetic Life Sciences Inc. (TSX: PLI) ... Pierre Laurin , President and Chief Executive Officer of ... Piper Jaffray 27 th Annual Healthcare Conference to be ... 2015. st , at 8.50am (ET) and ... the day. The presentation will be available live via a ...
(Date:11/24/2015)...  PDL BioPharma, Inc. (PDL) (NASDAQ: PDLI ) today ... and chief executive officer, will present at the 27 th ... New York City . The presentation will be ... at 9:30 a.m. EST. and go ... least 15 minutes prior to the presentation to allow for ...
(Date:11/24/2015)... According to two new studies, fewer men are having PSA ... doctors, scientists, and public health experts have been pushing for ... being done, will there be more men dying of ... "Despite the efforts made in regards to early detection for ... of death in men, killing approximately 27,500 men this year. ...
(Date:11/23/2015)... 23, 2015 China Cord Blood Corporation (NYSE: ... provider of cord blood collection, laboratory testing, hematopoietic stem ... its preliminary unaudited financial results for the second quarter ... 30, 2015. --> --> ... Revenues for the second quarter of fiscal 2016 increased ...
Breaking Biology Technology:
(Date:10/29/2015)... MINNETONKA, Minn. , Oct. 29, 2015   ... that supports the entire spectrum of clinical research, is ... the Minnesota High Tech Association (MHTA) as one of ... in the "Software – Small and Growing" category. The ... and individuals who have shown superior technology innovation and ...
(Date:10/29/2015)... October 29, 2015 NXTD ... company focused on the growing mobile commerce market ... that StackCommerce, a leading marketplace to discover and ... Wocket® smart wallet on StackSocial for this holiday ... or the "Company"), a biometric authentication company focused ...
(Date:10/26/2015)... , October 26, 2015 ... --> adds Biometrics Market ... 2021 as well as Emerging Biometrics ... reports to its collection of IT ... . --> ...
Breaking Biology News(10 mins):