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Northern Analysis: The Basics

s high sensitivity. The NorthernMax Kit is compatible with DNA, RNA or oligonucleotide probes labeled isotopically or nonisotopically.

The NorthernMax-Gly Kit incorporates many of the special features of our original NorthernMax Kit, but uses a glyoxal/DMSO loading solution for sample denaturation. Sample denaturation in glyoxal/DMSO instead of formaldehyde eliminates the need to pour and run gels in a fume hood, as well as the safety issues associated with use of formaldehyde. Also, RNA samples denatured with glyoxal may show sharper bands on Northern blots compared to samples denatured and run in the presence of formaldehyde. The streamlined NorthernMax-Gly procedure (adapted from Molecular Cloning, A Laboratory Manual, second edition, eds. Sambrook, Fristch and Maniatis) is more convenient and faster than the standard glyoxal gel protocol. A single reagent is used for sample denaturation/loading, the incubation time for sample denaturation has been reduced, and no recirculation of buffer is required during electrophoresis. The volume ratio of glyoxal denaturation solution to sample RNA is lower than in other published protocols, so that sample precipitation prior to gel loading is usually not required. Excellent results are typically seen using up to 30 g of RNA per lane. The NorthernMax-Gly Kit can be used with either radiolabeled or nonisotopically labeled RNA or DNA probe
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