Kit incorporates many of the special features of our
original NorthernMax Kit, but uses a glyoxal/DMSO loading
solution for sample denaturation. Sample denaturation in
glyoxal/DMSO instead of formaldehyde eliminates the need to
pour and run gels in a fume hood, as well as the safety issues
associated with use of formaldehyde. Also, RNA samples
denatured with glyoxal may show sharper bands on Northern
blots compared to samples denatured and run in the presence of
formaldehyde. The streamlined NorthernMax-Gly procedure
(adapted from Molecular Cloning, A Laboratory Manual,
second edition, eds. Sambrook, Fristch and Maniatis) is more
convenient and faster than the standard glyoxal gel protocol.
A single reagent is used for sample denaturation/loading, the
incubation time for sample denaturation has been reduced, and
no recirculation of buffer is required during electrophoresis.
The volume ratio of glyoxal denaturation solution to sample
RNA is lower than in other published protocols, so that sample
precipitation prior to gel loading is usually not required.
Excellent results are typically seen using up to 30 g of RNA
per lane. The NorthernMax-Gly Kit can be used with either
radiolabeled or nonisotopically labeled RNA or DNA probe