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Northern Analysis: The Basics

target=_top>Increasing Sensitivity in Northern Analysis with RNA Probes."

The quickest way to obtain high specific activity DNA probes is a 10-minute random-priming reaction using the DECAprime II or Strip-EZ DNA Kits. For more sensitive DNA probes, use the Strip-EZ PCR Kit to produce StripAble DNA probes by asymmetric PCR.


Denaturing Agarose Gel Electrophoresis

Once RNA samples are isolated, the first step in Northern analysis is denaturing agarose gel electrophoresis. Formaldehyde has traditionally been used as the denaturant, although the glyoxal system has several advantages over formaldehyde.

The NorthernMax Kit contains a complete set of RNase-free reagents including high quality agarose, gel loading solution, gel preparation and gel running buffers, transfer buffer, hybridization buffer, wash solutions and RNaseZap, a nontoxic ribonuclease decontamination solution for equipment and work surfaces for running formaldehyde-based Northern analysis. This kit is ideal for the investigator who wants to use familiar reagents while taking advantage of the NorthernMax Kit'
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