Navigation Links
Northern Analysis: The Basics

target=_top>Increasing Sensitivity in Northern Analysis with RNA Probes."

The quickest way to obtain high specific activity DNA probes is a 10-minute random-priming reaction using the DECAprime II or Strip-EZ DNA Kits. For more sensitive DNA probes, use the Strip-EZ PCR Kit to produce StripAble DNA probes by asymmetric PCR.


Denaturing Agarose Gel Electrophoresis

Once RNA samples are isolated, the first step in Northern analysis is denaturing agarose gel electrophoresis. Formaldehyde has traditionally been used as the denaturant, although the glyoxal system has several advantages over formaldehyde.

The NorthernMax Kit contains a complete set of RNase-free reagents including high quality agarose, gel loading solution, gel preparation and gel running buffers, transfer buffer, hybridization buffer, wash solutions and RNaseZap, a nontoxic ribonuclease decontamination solution for equipment and work surfaces for running formaldehyde-based Northern analysis. This kit is ideal for the investigator who wants to use familiar reagents while taking advantage of the NorthernMax Kit'
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Related biology technology :

1. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
2. Array Analysis: The Basics
3. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
4. Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms
5. Nuclease Protection Assays: The Basics
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Northern Analysis The Basics

(Date:7/1/2015)... InferMed , s ... will augment Elsevier ... olutions suite   Elsevier , a world-leading ... announced today the acquisition of  InferMed , a ... InferMed,s Arezzo technology supports clinicians in choosing the most ...
(Date:7/1/2015)... NEW YORK , July 1, 2015 /PRNewswire/ ... G ), a global leader in designing, ... the Aecus Innovation Award in recognition of their ... operational efficiencies in UCB,s global business services (GBS) ... acknowledge service providers and clients that work together ...
(Date:6/30/2015)... , June 30, 2015 /PRNewswire/ - Resverlogix ... of common shares of Resverlogix have approved the ... Eastern Capital Limited of 5,600,000 units at a ... Placement"), each unit being comprised of one common ... aggregate proceeds of approximately CAD$15 million; and (ii) ...
(Date:6/30/2015)...  Juniper Pharmaceuticals, Inc. (Nasdaq: JNP ), a ... address unmet medical needs in women,s health, today ... present at the Cantor Fitzgerald Inaugural Healthcare Conference ... 4:45 PM EDTLocation: , Le Parker Meridien Hotel, ... www.juniperpharma.com, under  ,Investor, or  click here ...
Breaking Biology Technology:Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 2Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 3Elsevier Acquires InferMed, Provider of Clinical Decision Support Technology 4UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 2UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 3UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 4UCB and Genpact Receive Aecus Innovation Award for Success in Global Business Services 5Resverlogix Corp. Shareholders Approve Matters Relating to Licensing and Equity Arrangement 2Resverlogix Corp. Shareholders Approve Matters Relating to Licensing and Equity Arrangement 3Juniper Pharmaceuticals to Present at Cantor Fitzgerald Inaugural Healthcare Conference on July 8, 2015 2
... , , ... Calibration of a dissolution apparatus is necessary to ... and to allow comparison between batches and between laboratories. USP ... in USP Dissolution. 1 Each individual ...
... INTRODUCTION , ... bacterial enzymes which hydrolyze β -lactam antibiotics. The majority of β ... successful approach to combating the serine active site β -lactamases is ... combination with a β -lactam antibiotic 4 . , There is ...
... Lam, B.S., ... , , INTRODUCTION , ... flu-orogenic peptide substrates. Enzyme selectivity is obtained by choosing a ... particular enzyme. The chromophore or fluorophore is attached near the ...
Cached Biology Technology:Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 2Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 3Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 4Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 5Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 6Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 7Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 8Using the SpectraMax Plus for USP Dissolution Calibration (MaxLine Application Note #27) 9A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20) 2A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20) 3A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20) 4A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20) 5A Microtiter-based Assay for the Determination of ID50s of b-lactamase Inhibitors Employing Reporter Substrates Detected at UV or Visible Wavelengths (MaxLine Application Note #20) 6Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35) 2Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35) 3Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35) 4Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35) 5Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35) 6
(Date:6/30/2015)... -- Genisphere announced Tom Bliss ... develop Genisphere,s therapeutics capabilities and portfolio. Mr. Bliss comes ... of the last 25 years in leadership positions at ... & Johnson. Tom started his career in Investment Banking, ... He graduated from Dartmouth College with a BA in ...
(Date:6/25/2015)...  TAKE Solutions Ltd., a global business technology solutions ... Patent and Trademark Office (USPTO) for its "Method for ... Clinical Accelerators to reduce the time taken to standardize ... without the accelerators), thus reducing time to market. ... the heart of the patented Clinical Data Standardization Process ...
(Date:6/24/2015)... Biometry authentication provider KeyLemon today announced ... one face in. Logo - ... in, entering the expanding biometric authentication market in ... answer to the password problem. With advanced face ... KeyLemon, one face in allows users to securely ...
Breaking Biology News(10 mins):Genisphere Appoints New CEO, Thomas Bliss 2Genisphere Appoints New CEO, Thomas Bliss 3TAKE Solutions Awarded Patent By USPTO 2KeyLemon Announces Partnership With Biometric Password Solution onefacein 2
... the soil can transform the most commonly used flame retardant ... could be harmful to humans, according to a new laboratory ... Chemical Society journal, Environmental Science & Technology . The ... issue of the journal. , The finding, by a team ...
... nicotine is a key to developing treatment for this ... neural machinery by which nicotine switches on the brain's ... 15, 2006, Neuron, Jean-Pierre Changeux of CNRS, Coll├Ęge de ... Pasteur and CNRS and colleagues revealed key details of ...
... demonstrated for the first time the ability to ... images for "fly-through" and "fly-around viewing" of cancer ... study in the July issue of the Journal ... meld functional data with accurate anatomical information of ...
Cached Biology News:Microbes transform 'safest' PBDEs into more harmful compounds 2Dissecting the machinery of nicotine's reward 2Coming soon: 3-D imaging that flies 'through' and 'around' cancer 2Coming soon: 3-D imaging that flies 'through' and 'around' cancer 3
Hamster serum...
... Reactive Dye Pack, 12 x Cy5. ... optimized for microarray labeling.Higher reactivity Cy3 ... dye per vial, at > 75% ... dispensed, and packed in foil packs ...
Collected from sexually mature, mixed sex, mixed breed dogs. Serum from specific breeds available by request....
... EPS 301 Power Supply, 1. ... maximum.Constant voltage or constant current modes.Single-unit ... precision and reproducibility.Suitable for submarine, mini ... as well as semidry and mini ...
Biology Products: