Navigation Links
Northern Analysis: The Basics

probes, and that RNA probes provide an additional 10-fold increase in sensitivity. RNA probes have the added advantage that they can be hybridized and washed under more stringent conditions, which results in lower background and fewer problems with cross-hybridization. For these reasons, Ambion has long been a proponent of using RNA probes for assaying Northern blots, although the use of ULTRAhyb Ultrasensitive Hybridization Buffer (see below) drastically reduces the disparity in sensitivity between RNA and DNA probes. Both types of probes can detect fewer than 100,000 molecules on a blot with ULTRAhyb, making the decision to use RNA or DNA probes primarily a matter of preference for a particular labeling technique.

RNA probes can be produced by in vitro transcription reactions using Ambion's MAXIscript or Strip-EZ RNA Kits. Strip-EZ RNA probes include a modification that allows them to be easily removed or "stripped" off blots, in contrast to standard RNA probes. Isotopic or nonisotopic labeled nucleotides can be incorporated directly during synthesis with either kit, or the RNA can be synthesized unlabeled and subsequently treated with Psoralen-Biotin to produce biotinylated probes for blot hybridizations. For more information about using RNA probes in Northern analysis, see "'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Related biology technology :

1. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
2. Array Analysis: The Basics
3. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
4. Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms
5. Nuclease Protection Assays: The Basics
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Northern Analysis The Basics

(Date:12/24/2014)... On Friday, December 19, 2014, President ... and Continuing Resolution Appropriations Act of 2015, which ... eligible to receive funding through the Congressionally Directed ... of Defense (DoD). The Hydrocephalus Association (HA), working ... is celebrating this victory for the over 1 ...
(Date:12/24/2014)... December 23, 2014 Nashville Fertility Center ... elected treasurer for the American Society for Reproductive Medicine ... and research in reproductive medicine. In his new ... on the executive board and will have a voice ... Hill has actively supported ASRM since 1984 when he ...
(Date:12/24/2014)... Dec. 23, 2014 China Biologic Products, ... a leading fully integrated plasma-based biopharmaceutical company in ... its majority-owned subsidiary, Shandong Taibang Biological Products Co. ... from the China Food and Drug Administration (the ... As previously disclosed in the Company,s public filings, ...
(Date:12/24/2014)... LOUIS , Dec. 23, 2014 ... today that the waiting period under the Hart-Scott-Rodino ... the acquisition expired on December 22, 2014, thereby ... review requirement for the acquisition of the Company ... U.S. antitrust clearance satisfies another condition to closing ...
Breaking Biology Technology:Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 2Nashville Fertility Center Announces Dr. George A. Hill Selected to Serve as ASRM Treasurer for 2014-15 3China Biologic Receives GMP Certification for New Coagulation Factor Facility 2China Biologic Receives GMP Certification for New Coagulation Factor Facility 3Acquisition of Sigma-Aldrich by Merck KGaA, Darmstadt, Germany, Receives U.S. Antitrust Clearance 2Acquisition of Sigma-Aldrich by Merck KGaA, Darmstadt, Germany, Receives U.S. Antitrust Clearance 3
... 2011 Psoriasis is a persistent, long-lasting chronic skin ... more than 125 million individuals.  For some, it is ... On a continuing quest to find more effective ... Journal of Investigative Dermatology reported that the ...
... 14, 2011 Merriman Capital, ... (NASDAQ: MERR ), today announced that it ... Circadian Technologies Limited (ASX: CIR; OTCQX: CKDXY) as it ... http://photos.prnewswire.com/prnh/20100914/SF64919LOGO ) Circadian is an Australian biotechnology ...
... June 14, 2011 OncoSec Medical Inc. (OTCBB: ... to treat solid tumor cancers, announced today two ... the clinical operations of OncoSec,s ElectroOncology therapies. ... Dhillon, OncoSec,s President and CEO, said, "OncoSec has ...
Cached Biology Technology:Actinobac Biomed, Inc. Demonstrates In Vivo Efficacy for Psoriasis Treatment 2Merriman Capital Announces Sponsorship of Circadian Technologies on OTCQX 2Merriman Capital Announces Sponsorship of Circadian Technologies on OTCQX 3OncoSec Announces Clinical Leadership Appointments 2OncoSec Announces Clinical Leadership Appointments 3OncoSec Announces Clinical Leadership Appointments 4
(Date:12/10/2014)... CIE San Diego has launched an innovative ... tissue that enhances care coordination among social service and ... information; earned a second $1 million grant from the ... aging in community and; will be recognized as a ... 4-6p. CIE San Diego today announced ...
(Date:12/10/2014)... California , December 9, 2014 ... with ITN,s BCARD Platform   ... cloud-based enterprise meeting scheduling solutions for business-to-business (B2B) ... the world leader in mobile near-field communication (NFC), ...      (Logo: http://photos.prnewswire.com/prnh/20140612/691055) , ...
(Date:12/5/2014)... , Dec. 4, 2014  Tute Genomics, a ... $2.3 million in Series A1 funding led by UK-based ... also participated in the investment round. "We ... healthcare community adopts next-generation sequencing and seeks new approaches ... Reid Robison , MD MBA, and CEO of ...
Breaking Biology News(10 mins):Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 2Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 3Community Information Exchange San Diego Launches Pilot, Secures $1 Million Expansion Grant, and Becomes a "Live Well San Diego" Partner 4Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 3Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3
... showed that they could cut the lives of disease-carrying mosquitoes ... from fruit flies. Now, a new report in the December ... suggests that their strategy might do one better: The Wolbachia ... viruses that are a growing threat to humans, including those ...
... Scientists have shown for the first time that insects, ... They made the discovery thanks to high-speed video cameras ... that they used to film desert locusts stepping along ... new light on insects, ability to perform complex tasks, such ...
... Glaciers along the Gulf of Alaska are enriching stream and ... carbon contained in glacial runoff, researchers from four universities and ... issue of the journal Nature *. In ... Environmental Science Program at the University of Alaska Southeast, set ...
Cached Biology News:New tool in the fight against mosquito-borne disease: A microbial 'mosquito net' 2Ladder-walking locusts show big brains aren't always best 2Glacier melt adds ancient edibles to marine buffet 2
... the biological data hidden in your samples with ... allows you to create your own microarray designs ... your own pace and receive delivery of your ... is a secure, online array creation application tool ...
Acetylcholinesterase (human brain, AChE)...
... meets the demands of the routine trace ... from reliable, robust and simple to use ... in the most challenging sample matrices found ... suited to the toughest analytical challenges found ...
C1-inhibitor (C1-INH)...
Biology Products: