Navigation Links
Northern Analysis: The Basics

.g., cDNA from a different species or genomic DNA fragments that might contain an intron) may be used as probes.

Despite these advantages, there are limitations associated with Northern analysis. First, if RNA samples are even slightly degraded, the quality of the data and the ability to quantitate expression are severely compromised. For example, even a single cleavage in 20% of 4 kb target molecules will decrease the returned signal by 20%. Thus, RNase-free reagents and techniques are essential. Second, a standard Northern procedure is, in general, less sensitive than nuclease protection assays and RT-PCR, although improvements in sensitivity can be achieved by using high specific activity antisense RNA probes, optimized hybridization buffers and positively charged nylon membranes. Sensitivity can be further improved with oligo dT selection for enrichment of mRNA, since physical constraints of gel electrophoresis and membrane transfer limit the amount of RNA that can be analyzed without loss of resolution and saturation of the transfer membrane. Ambion's NorthernMax reagents in combination with ULTRAhyb (see below) can dramatically increase the sensitivity of Northerns to the level of nuclease protection assays. A third limitation of Northern blotting has been the difficulty associated with multiple probe analysis. To detect more than one message, it is usually necessary to strip the initial probe before hybridizing with a second
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Related biology technology :

1. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
2. Array Analysis: The Basics
3. Real-Time PCR/Melt-Curve Analysis: SNP Detection With FRET, Rev A
4. Fine Tuning Your Data Analysis: Tunable Parameters of the Affymetrix Expression Analysis Statistical Algorithms
5. Nuclease Protection Assays: The Basics
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Northern Analysis The Basics

(Date:7/31/2015)... ... July 31, 2015 , ... Global Resorcinol Market ... industry insights on the global resorcinol industry. The report highlights the various growth ... market scenarios and analysis of primary and secondary inputs from the existing players ...
(Date:7/30/2015)... ... ... Ralco is honored to announce that it is the primary sponsor of ... 5-9 in Marshall. The Ralco Enrichment Center is an interactive experience where the public ... daily lives. This unique exhibit also features a birthing center for fair goers to ...
(Date:7/30/2015)... France and Dallas, TX, USA (PRWEB) , ... ... ... of innovative nano and microsystems, has appointed Francois Vieillard as Sales & Marketing ... Marketing at executive positions, Francois Vieillard will be responsible for reinforcing Tronics’ business ...
(Date:7/30/2015)... San Diego , CA (PRWEB) , ... July ... ... Biopharma, announced the successful completion of a field clinical study of its canine ... being developed together with Aratana Therapeutics (Kansas City, KS) and will be marketed ...
Breaking Biology Technology:Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 2Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 3Resorcinol Market to Grow at 6% CAGR to 2019 Says New Research Report at ReportsnReports.com 4Ralco Announces Primary Sponsorship of Ralco Enrichment Center at Lyon County Fair 2Tronics appoints Francois Vieillard as Sales and Marketing Director 2VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 2VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 3
... 2 Sicel Technologies, Inc., announced today that ... bankruptcy. Effective August 31, 2010, Paul McDaniel became ... the company. Currently, Sicel,s operations are continuing and ... returned to work.Media Contact: Lisa P. Sumner, Esq., ...
... Cell Treatments -- SHENZHEN, China, Sept. 2 /PRNewswire-Asia/ -- ... ... ... // Results are keyed by longUrl, so we need to grab the first one. for (var r ...
... growing interest in using nanoparticles for everything from ... the need to understand the environmental, health and ... at the National Institute of Standards and Technology ... nanocrystals that will enable studies of certain physical ...
Cached Biology Technology:New Research Demonstrates Safety of Cord-blood-derived Stem Cell Treatments 2New Research Demonstrates Safety of Cord-blood-derived Stem Cell Treatments 3New Research Demonstrates Safety of Cord-blood-derived Stem Cell Treatments 4New Research Demonstrates Safety of Cord-blood-derived Stem Cell Treatments 5New Research Demonstrates Safety of Cord-blood-derived Stem Cell Treatments 6New Research Demonstrates Safety of Cord-blood-derived Stem Cell Treatments 7New Research Demonstrates Safety of Cord-blood-derived Stem Cell Treatments 8The perfect nanocube: Precise control of size, shape and composition 2
(Date:7/8/2015)... 2015 Summary Pancreatic cancer is the ... fatal, with a mortality rate of 10.9 deaths per ... cancer patients has highlighted a significant need for new ... met by the current market. A highly active ... varying molecule types and mechanisms of action, which provides ...
(Date:7/7/2015)... , July 7, 2015  Based on its ... & Sullivan recognizes Credence ID, LLC with the ... Company of the Year Award. Credence ID has ... in its mission of offering enrollment and identification ... As Credence ID was formed by experts from ...
(Date:6/30/2015)... , June 30, 2015 To bolster its efforts ... HYPR Corp. announced today the addition of two new team ... as board advisor and David Raviv will act ... underscore HYPR Corp.,s commitment to providing the most secure solutions ... Sirota co-founded Layer 7 Technologies, a provider of security ...
Breaking Biology News(10 mins):Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 2Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 3Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 2Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 3Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 4Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 5HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 3
... typically associated with trees; they can take centuries to grow. However, ... in the Proceedings of the National Academy of Sciences ... growing faster than they have in the past 225 years. The ... to ,climate change. For more than 20 years forest ecologist ...
... day care workers and kindergarten teachers tend to offer young humans ... sharing just comes naturally. In fact, according to a pair ... like bonobos never seem to learn how not to share. ... by physical aggression if necessary. While chimps will share as youngsters, ...
... one day walk on walls, like Spider-Man? A palm-sized device ... adhesive bond just might make it possible. The rapid ... gloves that stick and unstick to walls, or Post-it-like notes ... chemical and biomolecular engineering, who invented the device with Michael ...
Cached Biology News:Ecologists discover forests are growing faster 2Ecologists discover forests are growing faster 3'Peter Pan' apes never seem to learn selfishness 2'Peter Pan' apes never seem to learn selfishness 3New adhesive device could let humans walk on walls 2
...
Mouse polyclonal antibody raised against a partial recombinant DDEF2. NCBI Entrez Gene ID = 8853...
Mouse monoclonal antibody raised against a full length recombinant SCGB3A2. NCBI Entrez Gene ID = SCGB3A2...
Mouse monoclonal antibody raised against a partial recombinant QARS. NCBI Entrez Gene ID = QARS...
Biology Products: