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Northern Analysis: The Basics

condary detection. The blot is then incubated in Probe Degradation Buffer, which cleaves the probe at the site of the modified nucleotides, reducing the RNA or DNA probe to short oligonucleotides. The degraded probe is easily dissociated from its target during the mild degradation wash (10 min., 68C), allowing the blot to be probed again without residual signal or loss of bound target.

Strip-EZ StripAble Probe Synthesis and Removal Kits are available for the synthesis of DNA probes by random-priming (Strip-EZ DNA Kit) or asymmetric PCR (Strip-EZ PCR Kit) and the synthesis of RNA probes by in vitro transcription (Strip-EZ RNA Kit).


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Ordering Information
Cat# Product Name Size 1308 MAXIscript SP6 Kit 30 rxns 1310 MAXIscript SP6 Kit 100 rxns 1312 MAXIscript T7 Kit 30 rxns<
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