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Northern Analysis: The Basics

RNA and DNA probes. The NorthernMax Kits have been optimized in conjunction with the BrightStar BioDetect Kit for ultrasensitive nonisotopic Northern blots with a high signal-to-noise ratio and low background. This optimization of the Northern blotting method also yields excellent results with radiolabeled probes.


Stripping and Reprobing

Standard methods for removing probes from blots to allow subsequent hybridization with a different probe often include harsh treatments with boiling 0.1% SDS or even autoclaving. These procedures not only damage the membrane, but can also damage the target RNA bound to the membrane, potentially decreasing the signal obtained from the next hybridization. However, probes prepared with one of the Strip-EZ Probe Synthesis and Removal Kits can be removed with mild washing conditions, allowing a blot to be stripped and re-hybridized up to 10 times without a significant loss of signal or damage to the membrane. The Strip-EZ Kits include the reagents necessary to synthesize a probe containing a specific modified nucleotide. The modification does not affect the hybridization characteristics of the probe, but enables selective degradation of the probe molecule using a specially designed buffer. In a typical experiment, a StripAble probe is synthesized, incubated with a blot, washed, and the probe signal is detected via autoradiography or nonisotopic se
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