from coating the membrane. Good blocking is necessary to
minimize background problems.
The NorthernMax and NorthernMax-Gly kits include
Hybridization Buffer, which can be used for both prehybridization and
hybridization. Although double-stranded DNA probes must be denatured prior
to use, RNA probes and single-stranded DNA probes can be diluted in a
small amount of ULTRAhyb and then added to the prehybridized blot. ULTRAhyb
can increase sensitivity up to 100-fold compared to other hybridization
solutions (Figure 2) by pushing hybridization to completion without increasing
background. As few as 10,000 molecules can be detected. Because ULTRAhyb
maximizes blot sensitivity, hybridization can be performed in just 2 hours
for many messages. The largest increase in sensitivity is seen using random-primed
DNA probes and ULTRAhyb. Under these conditions, signals are as intense
as those seen with RNA probes.
Figure 2. ULTRAhyb versus a standard hybridization
buffer using DNA and RNA probes. Identical
Northern blots of indicated amounts of mouse thymus total RNA were hybridized
overnight with radiolabeled StripAble DNA or RNA probe. The blots were
incubated with probe in either ULTRAhyb or standard hybridization buffer
as indicated. Standard low and high stringency SSC/SDS washes were used
to remove nonspecifically bound probe. Blots were exposed to film for
2.5 hours with a single intensifying screen.
(Date:12/24/2014)... AUSTIN, Texas , Dec. 24, 2014 /PRNewswire/ ... company focused on gynecologic disease, announced today that ... with investors including Oracle Investment Management, Jack ... Vermillion directors. Total proceeds were $10.5 million, before ... working capital and general corporate purposes. ...
(Date:12/24/2014)... 23, 2014 “Preparative & Process ... Flash), Consumables (Columns, Solvents, Buffers, Valves, Guages, Seals), ... Agriculture) - Forecasts to 2019” provides a detailed ... current market trends, and strategies impacting the preparative ... and forecasts of the revenue and share analysis. ...
(Date:12/24/2014)... Earlier this year in a June 24 international ... Adult Stem Cell Technology Center, LLC ( ASCTC ) ... unique property of adult tissue stem cells. His ... the Past, Important for the Future,” embodied the essence ... the address at the 4th World Congress on Cell ...
(Date:12/24/2014)... 2014 On Friday, December 19, ... the Omnibus and Continuing Resolution Appropriations Act of ... a condition eligible to receive funding through the ... the Department of Defense (DoD). The Hydrocephalus Association ... Capitol Hill, is celebrating this victory for the ... Breaking Biology Technology:Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 2Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 4Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 2Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 4Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2
... ISO 9001:2000 Certified USDA Research Registered OLAW ... Training AALAS Certified Technicians ... GLP Documentation Upon Request Focus ... for all your custom immunology service needs. ...
Applications: Western blotting ...