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Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged ,,, proteins

Ni-NTA Superflow Columns enable up to 24 automated large-scale 6xHis-tagged protein preparations to be performed in parallel on the BioRobot 3000 workstation. Using cleared lysates from bacterial cultures as starting material, up to 15 mg protein per column can be purified under native or denaturing
conditions using ready-to-run protocols. The increase in yield means that more experiments can be performed using a single batch of protein, with enough protein for a structural determination being obtained from a single column.

Ni-NTA Superflow Columns offer:
  • High yields - up to 15 mg highly pure 6xHis-tagged protein per column
  • Automated purification - multiparallel processing of up to 24 samples on the BioRobot 3000 workstation
  • High purity - cross-contaminationCfree processing with high column-to-column reproducibility
  • Convenience - ready-to-run protocols for purification under native or denaturing conditions
Purification using proven 6xHis-tagNi-NTA technology Ni-NTA Superflow Columns
Automated Procedure
6xHis-tagged proteins are efficiently purified on Ni-NTA Superflow Columns using the highly selective interaction between an affinity tag of 6 consecutive histidine residues (the 6xHis tag) and nickel-nitrilotriacetic acid (Ni-NTA). The four chelating sites of NTA bind nickel ions more tightly than alternative metalchelation purification systems that use ligands with only 3 chelating sites. The extra chelating site of N
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