Navigation Links
New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays

between replicates. Results are shown in Figure 6.

The results show that for both MAS 4.0 and MAS 5.0, over 95% (at some concentrations 100%) correct No Change calls are made.

The concordance between Increase calls made by MAS 4.0 and MAS 5.0 are shown in Figure 7. The same transcript group was compared in pairs of experiments to evaluate the calls for a 2-fold and 4-fold change in concentration. For example, in the Latin Square represented in Figure 1, transcript group B was compared between Experiments 1 and 2, then 2 and 3, then 3 and 4, and so on, to determine the fraction correctly assigned an Increase call at the expected 2-fold change. For the 4-fold change, transcript group B was compared between Experiments 1 and 3, 2 and 4, 3 and 5 etc., such that all transcript groups were evaluated.

In this data set, MAS 5.0 shows greater accuracy than does MAS 4.0 at both 2-fold and 4-fold Increase levels. For example, at the 2 pM spike in Figure 7B, MAS 5.0 gives a nearly 10% greater fraction of correct Increase calls than does MAS 4.0. The drop in accuracy seen at very high concentration is due to the saturation of probe sets by the vast excess of spiked transcripts.

To understand the difference in the performance of calls generated by MAS 4.0 and MAS 5.0 on a biological sample, we assessed the results using an independent method. We used the power of replicates and the wellknown student’s t-test as a reference.

We analyzed a number of replicates in two tissues and then asked, “How well do the results from any single comparison match results from the group as a whole?”

The group was built from two sets of experiments—mouse brain and mouse heart—containing six replicates for each tissue. Each replicate was compared to every other replicate with both MAS 4.0 and MAS 5.0 (36 comparisons of 12488 probe sets). The t-test was then used to
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Versatile Reporter Vectors for Monitoring Viral Transduction
2. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
3. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
4. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
5. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
6. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
7. Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation
8. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
9. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
10. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
11. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
Post Your Comments:
(Date:8/31/2015)... Aug. 31, 2015  For people with Down syndrome, ... day. The biotechnology company, located in the Science + ... the best potential therapies yet for people with Down syndrome ... Correction of Down syndrome and Edwards syndrome aneuploidies in ... DNA Research . Elixirgen plans to develop this technology ...
(Date:8/31/2015)... , Aug. 31, 2015  RXi Pharmaceuticals ... focused on discovering and developing innovative therapies primarily ... announced that the 30-day review period following the ... for ocular indications with the U.S. Food and ... is now active. Logo -  ...
(Date:8/31/2015)... and SHANGHAI , Aug. 31, ... company developing next-generation antibiotics, today announced positive top-line ... for its lead drug candidate MRX-I. MRX-I is ... bacteria such as MRSA and VRE, while offering ... therapeutic option than currently available oxazolidinone agents. ...
(Date:8/31/2015)... 2015 LabStyle Innovations Corp. (OTCQB: DRIO), ... announced it recently closed a round of funding ... to support the global rollout and initial U.S. ... diabetes management solution that includes novel software applications ... monitoring device, and for working capital purposes. The ...
Breaking Biology Technology:New Down syndrome therapy discovered 2RXi Pharmaceuticals Announces Advancements in its Ophthalmology Clinical Program with RXI-109 2RXi Pharmaceuticals Announces Advancements in its Ophthalmology Clinical Program with RXI-109 3RXi Pharmaceuticals Announces Advancements in its Ophthalmology Clinical Program with RXI-109 4MicuRX Reports Positive Top-Line Results In Phase 2 Clinical Trial For Novel Antibiotic MRX-I In Complicated Skin And Soft Tissue Infections 2LabStyle Innovations Closes $2.5 Million Funding to Support Global Rollout of Dario Diabetes Management Solution 2LabStyle Innovations Closes $2.5 Million Funding to Support Global Rollout of Dario Diabetes Management Solution 3LabStyle Innovations Closes $2.5 Million Funding to Support Global Rollout of Dario Diabetes Management Solution 4
... Molecules through ... Gene-based Search, ... the addition of 1,300 bioactive small molecules to,its gene-based search ... data on the functional relationship between,bioactive small molecules and genes ...
... Imaging Biomarker Production Facility to Enable Healthcare ... Providers Access ... Siemens Healthcare, (http://www.siemens.com/healthcare ) today announced its first ... facility will,enable the healthcare providers in and around Chennai ...
... MD, Executive Vice,President and Physician-in-Chief at The University ... the 40th President of the Society of,Gynecologic Oncologists ... 9-12, 2008 in Tampa, Florida., Dr. Burke ... in New Orleans, Louisiana. He served both his ...
Cached Biology Technology:Sigma-Aldrich Partners With Sunset Molecular Discovery to Expand Your Favorite Gene Search Tool 2Siemens to Launch PETNET Services in India 2Siemens to Launch PETNET Services in India 3Thomas W. Burke, MD, Elected 40th President of the Society of Gynecologic Oncologists 2Thomas W. Burke, MD, Elected 40th President of the Society of Gynecologic Oncologists 3
(Date:8/6/2015)... 2015  Today, U.S. Congressman Mike Honda ... to learn firsthand how Silicon Valley technologies can ... a world leader in multi-factor identity management and ... federal agencies and commercial organizations achieve new levels ... "The vast majority of network breaches can be ...
(Date:8/5/2015)... MOUNTAIN VIEW, Calif. , Aug. 5, 2015 /PRNewswire/ ... it exhibits continuous growth in applications, penetration into newer ... year after year. The global biosensors space has seen ... having exited the market so far. (Photo ... from Frost & Sullivan, Analysis of the Global ...
(Date:7/31/2015)... 31 de julio de 2015 BGI llevará a ... ) del 22 al 25 de octubre de 2015, ... La conferencia celebra su décimo aniversario este año. Desde ... una de las reuniones anuales más influyentes del mundo ... de las conferencias científicas más dinámicas, entusiastas y amenas. ...
Breaking Biology News(10 mins):Rep. Honda to Visit Crossmatch in Redwood City 2Innovative Biosensors Incite Use in Non-Traditional Applications 2Innovative Biosensors Incite Use in Non-Traditional Applications 3Innovative Biosensors Incite Use in Non-Traditional Applications 4La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 2La 10ª Conferencia Internacional sobre Genómica (ICG-10) comienza en octubre 3
... is a well-known cause of cardiovascular disease, research from the ... revealed that the body,s obesity hormones adiponectin - are ... Our skeleton is more than just bones, vertebrae ... is constantly linked to our brain, our muscles and our ...
... LANSING, Mich. Discovering new treatments and battling dangerous ... researchers targeting the genetic disorder cystic fibrosis with projects ... research studies, with support from a $110,000 grant from ... and Translational Sciences Institute, expand the relationship between the ...
... 1st, 2011) Policy decisions and poor management have ... national security, according to an article available today in ... Scientists, published by SAGE. The article calls into question ... scientists for the decline. , According to George Mason ...
Cached Biology News:4 projects target cystic fibrosis with Hunt for a Cure funds 2Structure, not scientists to blame for Los Alamos failings 2Structure, not scientists to blame for Los Alamos failings 3