Navigation Links
New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays

compared, the results are highly similar, with a regression ratio of 0.94. Similar results were obtained across multiple tissues and with replicates (data not shown). This similarity was also observed on arrays representing different organisms. The regression ratios for these comparisons were in the 0.92-0.93 range for M. musculus, A. thaliana and S. cerevisiae , and 0.96-0.97 for D. melanogaster and E. coli .

The occurrence of negative values in MAS 4.0, seen in the two left quadrants in Figure 2B, has been eliminated from MAS 5.0. The corresponding output in MAS 5.0, termed “Signal” has no negative and zero values as is evident in the lower two quadrants of Figure 2B.

Added Statistical Quality Measures and Tunable Parameters

An added benefit of the statistical algorithms in MAS 5.0 is the inclusion of probability values (statistical significance) associated with detection and comparison calls. This additional metric allows users to assess the significance of results, and if desired, adjust the balance between sensitivity and specificity. For example, higher confidence in expression calls (greater specificity) can be achieved by accepting fewer Present calls (lower sensitivity). Conversely, greater sensitivity may be achieved at the expense of lower specificity. The inverse relationship between sensitivity and specificity in MAS 5.0 was examined by monitoring detection calls in the set of experiments described below. As shown in Figure 3, a greater number of accurate Present calls is made by MAS 5.0 at concentrations below 4 pM using the default p-value setting. However, the small number of false positives is also slightly greater for MAS 5.0 in this experiment, as seen at the 0 pM concentration. The sensitivity and specificity may be varied in a predictable fashion by varying the default setting for p-value cutoff.

Figure 4
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Versatile Reporter Vectors for Monitoring Viral Transduction
2. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
3. Monitoring Microbial Populations Using Real-Time qPCR on the MJ Research Opticon 2 System
4. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
5. Direct Monitoring of Solid-Phase Peptide Synthesis by MALDI-TOF MS
6. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
7. Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation
8. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
9. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
10. High-Level Expression of Peanut Allergens Affected by Rare Codon Usage
11. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
Post Your Comments:
(Date:5/21/2015)... Germany (PRWEB) May 21, 2015 ... that its manufacturing facility in Worms, Germany has ... an independent subsidiary of the International Pharmaceutical Excipient ... Grace facilities that produce its SYLOID® FP brand ... received GMP certification, following the Curtis Bay, Maryland ...
(Date:5/21/2015)... 21, 2015  CytRx Corporation (NASDAQ: CYTR ... in oncology, today announced positive updated results from ... for the treatment of unresectable glioblastoma multiforme (GBM), ... multisite trial is designed to investigate the preliminary ... tumors have progressed following prior treatment with surgery, ...
(Date:5/21/2015)... , May 20, 2015 Research and ... the addition of the "2015 Global Survey ... their offering. The primary goal of ... flow cytometry instruments and reagents. Key information the ... the selection of flow cytometers, predominantly used applications ...
(Date:5/21/2015)... 21, 2015 Veolia’s environmental monitoring ... North American distribution agreement with VWR to distribute ... With more than 160 years of experience, VWR, ... for laboratory and production facilities, has cultivated a ... differentiated services to enable science. Endetec’s TECTA™ ...
Breaking Biology Technology:Grace European Facility Receives GMP Excipient Certification for SYLOID® FP Silica Gel 2CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 2CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 3CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 4CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 5CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 6CytRx Reports Positive Updated Phase 2 Aldoxorubicin Clinical Trial Results in Glioblastoma Multiforme (Brain Cancer) 7Global Survey on Flow Cytometry Adoption Trends 2015 2Veolia’s TECTA™ B16 Instrument for E. Coli Detection is Offered Exclusively Through VWR 2
... Being the right size and existing in the limbo between ... secrets to improving the efficiency of chemical catalysts that can ... matter is in this transitional state, a catalyst can achieve ... size and temperature, according to a pair of Duke University ...
... Oct. 21 Transgenomic, Inc. (OTC Bulletin Board: ... has joined the company as Medical Director for Laboratory ... be responsible for leading the strategic vision and managing ... Dr. Johnson brings to Transgenomic nearly ...
... CAMBRIDGE, Mass., Oct. 21 Sermo ( http://www.sermo.com ), ... free Sermo Report titled, " Physician Participation in the ... and participation rates in the FDA-required Erythropoiesis-Stimulating Agents (ESAs) ... According to the results, 83% of Hematologists/Oncologists view the ...
Cached Biology Technology:Smaller is better in the viscous zone 2Dr. Eric Johnson Joins Transgenomic as Medical Director for Laboratory Services 2Dr. Eric Johnson Joins Transgenomic as Medical Director for Laboratory Services 3Dr. Eric Johnson Joins Transgenomic as Medical Director for Laboratory Services 4Sermo Report Indicates Possible Decline in Prescribing of Erythropoiesis-Stimulating Agents 2
(Date:5/8/2015)... 2015 Synaptics Inc. (NASDAQ: SYNA ), the ... of the executive management team will present at the following ... and Telecom Conference Date: May 18, 2015 Time: ... MA Cowen and Company Technology, Media ... Location: The New York Palace Hotel, New York, ...
(Date:4/27/2015)... , Apr. 27, 2015 NXT-ID, Inc. (NASDAQ: ... authentication company focused on the growing mobile commerce market, ... to pre-order customers the first week of May, 2015 ... month of May. Gino Pereira ... milestone for the company as Wocket® enters the consumer ...
(Date:4/17/2015)... BURNABY, Canada , April 17, 2015 /PRNewswire/ ... projects, growing security concerns, and technological advancement to ... Arabia until 2020   ... " Saudi Arabia Biometric Systems Market Forecast &  Opportunities, 2020 ", biometric ... is projected to register growth at CAGR ...
Breaking Biology News(10 mins):Synaptics to Present at Upcoming Investor Conferences 2Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 2Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 3Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 2Saudi Arabia Biometric Systems Market to Grow at 22% Through 2020, Says TechSci Research 3
... the mechanism by which the new genetically engineered vaccine ... Management who will test it in clinical trials early ... this vaccine has now been deciphered (Journal of Clinical ... the number one killer in adults. Moreover, multi-drug-resistant tuberculosis ...
... The Burnham Institute's Maurizio Pellecchia, Ph.D., has identified inhibitors ... could be developed into an emergency treatment for exposure ... the Proceedings of the National Academy of Sciences by ... June 20th. , Bacillus anthracis ("anthrax") is a proven ...
... Sultans of Soil, they're just clever Lab guys. A team ... week's Science Magazine with a new way to count bugs ... beneath our feet. , "Computational Improvements Reveal Great Bacterial Diversity ... Wolinsky and John Dunbar, of Los Alamos' Bioscience Division, describes ...
Cached Biology News:Anthrax inhibitors identified by Burnham team 2Anthrax inhibitors identified by Burnham team 3Comparison of Cocaine, Methamphetamine ‘Highs?Finds Differences 2
Rab GDP dissociation inhibitor beta...
... Drummond Scientific has a long and ... microinjection apparatus. Several years ago we ... displacement microdispenser was being modified to ... unit by redesigning the microdispenser to ...
Coating : Poly-D-Lysine ,Surface : ECM/attachment factors ,Surface area : 9.6 cm2 ,Dim nominal : 35 mm x 10 mm ,Vol working : 2.5-3.0 ml ...
Rabbit polyclonal to Synapsin II ( Abpromise for all tested applications). entrezGeneID: 6854 SwissProtID: Q92777...
Biology Products: