Navigation Links
New gravity-flow column gives faster and simpler purification of histidine-tagged proteins

A. Bergh, M. Carlsson, A. Karlsson, and H. Lindgren
GE Healthcare, Uppsala, Sweden


Histidine-tagged proteins* can be quickly and simply purified on His GraviTrap columns prepacked with Ni Sepharose 6 Fast Flow without the need for a pump or purification system. The protein binding capacity (approx. 40 mg/column) is among the highest available on the market, and separations can be completed in as little as 30 min. Large volumes of clarified or unclarified samples can easily be applied and the purified protein can be eluted in a small volume resulting in a highly concentrated target protein.

Introduction
Immobilized metal ion affinity chromatography (IMAC) is a widely used and highly effective technique that exploits the affinity of histidine-tagged proteins to metal ions attached to chelating separation media, such as Ni Sepharose 6 Fast Flow. His GraviTrap is a single-use column prepacked with Ni Sepharose 6 Fast Flow. His GraviTrap provides high protein binding capacity, short purification times, and simple operation.

The purification of histidine-tagged proteins on His GraviTrap can be divided into four stages: equilibration, sample application, washing, and elution (Fig. 1). Purifications can be performed either under native or denaturing conditions, and a number of additives can be used. In addition, new accessory products further increase speed and convenience.


His GraviTrap and accessories
His GraviTrap contains 1 ml of Ni Sepharose 6 Fast Flow, which delivers a protein binding capacity of approximately 40 mg histidine-tagged protein/column. The columns are made of bio-inert polypropylene, and special frits protect the medium from running dry during use. The column packaging can be converted into a stand (Workmate), and the plastic tray in this package can b
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
2. TSKgel ODS-100Z and TSKgel ODS-100V column series provides a universal solution for the analysis of samples containing acidic, basic, polar & non-polar compounds
3. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
4. Laser microdissection and nucleic acid purification - a Leica - QIAGEN cooperation
5. DirectPrep 96 Miniprep System for cost-effective, high-throughput plasmid DNA purification
6. Ni-NTA Superflow Columns - automated large-scale purification of 6xHis-tagged proteins
7. Walkaway automation for nucleic acid purification
8. DNA purification from pig ears to bird blood using proven DNeasy Technology
9. Addition of imidazole during binding improves purity of histidine-tagged proteins
10. 2100 expert software Powerful software for the analysis of RNA, DNA, proteins, and cells with the Agilent 2100 bioanalyzer
11. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
Post Your Comments:
(Date:9/29/2014)... the start of the military conflicts in Iraq and ... United States with traumatic brain injury caused by exposure ... improvised explosive devices, or IEDs. Symptoms of traumatic brain ... headaches and nausea, to more severe impairments in memory ... of Defense has recognized the critical importance and complexity ...
(Date:9/29/2014)... Sept. 29, 2014 Research and Markets ... Market by Product & Services, End-User , Application - ... The global oligonucleotide synthesis market is ... million in 2014, growing at a CAGR of 9.8% ... is categorized on the basis of products and services, ...
(Date:9/29/2014)... global challenges in food security, emerging diseases and ... study published online in Science Express ... University of Arizona College of Agriculture and Life ... team of nine scientists has reviewed progress in ... medicine and environmental management using approaches that consider ...
(Date:9/29/2014)... Research and Markets  has announced the ... and Gas Chromatograph Market by Type , Technology, Spectroscopy, ... & Forecast to 2013 - 2020" report to ... a process analytical instrument that provides chemical composition data ... way by which companies carry out their production and ...
Breaking Biology Technology:Modeling shockwaves through the brain 2Modeling shockwaves through the brain 3Modeling shockwaves through the brain 4Oligonucleotide Synthesis (Diagnostics, PCR, QPCR, Gene Synthesis, NGS, DNA, RNAi) Market - Global Forecast to 2019 2Oligonucleotide Synthesis (Diagnostics, PCR, QPCR, Gene Synthesis, NGS, DNA, RNAi) Market - Global Forecast to 2019 3Evolutionary biology: It's not just for textbooks anymore 2Evolutionary biology: It's not just for textbooks anymore 3Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 2Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 3Global Process Liquid Analyzer, Gas Analyzer, and Gas Chromatograph (PH/ORP, Chlorine, Oxygen, Carbon dioxide) Market - Trends & Forecast to 2020 4
... the appointment of James Stoppert as President and Chief Executive Officer ... ... EON) September 3, 2008 -- Segetis is the first and ... wide range of industrial chemicals and plastics that are ultimately derived ...
... China, Sept. 3 ,Acesys Pharmatech and Provid Pharmaceuticals ... expertise and project management,capabilities of Provid in the ... structure of Acesys in China., The combined ... of medicinal chemistry for clients in the pharmaceutical ...
... tested new molecular tools for stem cell research to direct ... development programmes. , A collaborative team of scientists from ... (NESCI) have developed two synthetic molecules which can be used ... into other forms of tissue. , Their use ...
Cached Biology Technology: Stoppert Named as President and CEO at Breakthrough Renewable Technology Firm Segetis, Inc 2Acesys and Provid Announce Alliance for Medicinal Chemistry Services 2New stem cell tools to aid drug development 2New stem cell tools to aid drug development 3
(Date:9/30/2014)... German . ... risky situations such as exposure to predators. Researchers from ... a long-term study on different populations of great tits ... ambient temperature. High metabolic rates and low temperatures were ... birds were more likely to approach potential predators. , ...
(Date:9/30/2014)... Coral reefs face a suite of perilous threats ... coastal development and climate change, fragile coral ecosystems ... Despite this trend, some species of corals surrounding ... a natural protector in their tropical environment: coral ... Natural History,s Smithsonian Marine Station scientist Seabird McKeon ...
(Date:9/30/2014)... -- Winners of The Economist,s prestigious Innovation awards 2014 ... place at the JW Marriott Hong Kong on Friday, October 10 ... their experiences and the lessons those hold for others. The awards, ... the past decade, will be presented at a ceremony in ... will be the first time the ceremony has come to ...
Breaking Biology News(10 mins):Risky metabolism 2Smithsonian scientists discover coral's best defender against an army of sea stars 2Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 2Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 3Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 4Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 5Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 6Hear from The Economist's 2014 Innovation Award Winners at Innovation Summit on October 10th in Hong Kong 7
... like AND and OR Boolean logic gates, producing an output ... in the May issue of Nature Chemical Biology , ... tiny circuits. Study leader Takanari Inoue, Ph.D., an assistant ... the Institute of Basic Biomedical Sciences, Center for Cell Dynamics ...
... of published studies on the impact of bark beetles ... more complete picture of the effect of this destructive ... a wide variety ofand often contradictoryconclusions about the bark ... researchers from the USDA Forest Service,s Pacific Southwest Research ...
... Calif.) Synthetic platelets have been developed by UC ... Research Institute and Sanford-Burnham Institute in La Jolla, Calif. ... Materials in a paper titled "Platelet Mimetic Particles ... the components of blood that allow it to prevent ...
Cached Biology News:Training cells to perform Boolean functions? It's logical 2Training cells to perform Boolean functions? It's logical 3New report examines effects of trees killed by bark beetles on wildfire 2UC Santa Barbara researchers develop synthetic platelets 2
Conveniently document gels, films and membranes with a high quality thermal printer. , Compact, footprint printer , Supplies 256 gray scale for high resolution, archive-quality thermal film prints...
... ICE-LAP3 Mch3 ... study of enzyme regulation and kinetics, cleavage of target ... 15,000 units/mg protein. One unit is defined ... nmol of Ac-DEVD-pNA Cat. No. 235400 per hour at ...
Request Info...
Bovine Coronary Artery Endothelial Cells (BCAEC) (>500,000 cells)...
Biology Products: