Navigation Links
New Yeast Cloning System for Producing Proteins with Native Amino Acid,,,Sequences

one agarose, the beads were washed and the fusion protein was eluted with 50 mM reduced glutathione. Samples of cells from induced conditions are shown (Figure 3A). Of the eluted GST-calmodulin fusion protein, 20 g was cleaved in a 60-l reaction volume using Stratagenes Enterokinase (figure 3B) at room temperature for 10 or 20 hours.

Acknowledgments

We would like to thank Peter Vaillancourt, Alan Greener and Denise Wyborski for discussions and suggestions and Cherie Dewar for technical assistance.

REFERENCES

  1. Lu, Q., Jerpseth, B., Sanchez, T., Bauer, J.C., and Greener, A. (1997) Strategies 10: 4-6.

  2. Aslanidis, C., and de Jong, P.J. (1990) Nucleic Acids Res. 18: 6069-6074.

  3. Huan, R.S., et al. (1992) Biotechniques 13: 515-518.

  4. Huan, R., and Moss, J. (1992) Gene 112: 37-43.


'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Epitope-Tagging Vectors for Functional Analysis in Yeast
2. Yeast Protein Production System Features High Yields and One-Step Purification
3. Electroporation of Yeast Artificial Chromosomes
4. New Automated Yeast Cell Counter Ends Tedium & Errors of Manual Counting
5. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation
6. Enhanced PCR Cloning System
7. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
8. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
9. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
10. QIAGEN PCR CloningPlus Kit
11. Cloning Based on Efficient Three-Fragment Assembly DNA Ligation
Post Your Comments:
(Date:7/11/2014)...  Researchers have pioneered a revolutionary new way ... called Virtual Finger, allows scientists to move through ... synapses using the flat surface of their computer ... studies orders of magnitude more efficient, saving time, ... many areas of experimental biology. The software and ...
(Date:7/10/2014)... 10, 2014 Women in the ... to an additional family planning option. Sayana® Press ... at all levels of the health system and ... a widely used contraceptive—Pfizer’s Depo-Provera® (depot medroxyprogesterone acetate)—with ... is the first of four African countries expected ...
(Date:7/10/2014)... Deep Knowledge Ventures last week sponsored ... Longevity Research’ and welcomed a host of London-based ... Innovation Center for the event, organised by the ... The meeting highlighted the need for both philanthropic ... disease, as well as the crucial role of ...
(Date:7/10/2014)... Mass. (PRWEB) July 10, 2014 Terascala, ... today announced that Alan Swahn, a former vice president ... executive team as vice president of marketing. Terascala’s software ... Dell and NetApp create the highest performance and most ... been appointed to guide Terascala’s channel expansion and broaden ...
Breaking Biology Technology:Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 2Virtual Finger Enables Scientists To Navigate And Analyze 3D Images Of Complex Biological Structures 3Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 2Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 3Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 4Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 5Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 6DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 2DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 3DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 4DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 5DKV Sponsors Forum on Commercialising Longevity Research; Brings Together Scientists, Investors, Entrepreneurs and Celebrities 6Terascala Expands Executive Team 2Terascala Expands Executive Team 3
... Madison Internet service provider SupraNet plans to ... the Aidmatrix Foundation, a global disaster-relief firm run by ... CEO Bryan Chan said in a press release that ... number of recent disasters the charity is responding to, ...
... of Wisconsin-Madison MBA program was ranked 51st in ... U.S. public university programs by the London Financial Times ... , ,These rankings are based on alumni careers and ... data provided by the schools and a survey of ...
... development assessment group for its high quality of life and ... States, released last week by the non-profit Corporation for Enterprise ... in business vitality and development capacity, based on 2004 data. ... subcategories that made up those three overall marks. , ,Perhaps ...
Cached Biology Technology:
(Date:7/11/2014)... 2014 Researchers have pioneered a revolutionary new ... technology, called Virtual Finger, allows scientists to move ... and synapses using the flat surface of their ... imaging studies orders of magnitude more efficient, saving ... across many areas of experimental biology. The software ...
(Date:7/11/2014)... hippocampus, the marginal division of the striatum is ... the impact degree of substance P in the ... Yan Yu and his team, College of Biophotonics, ... staining, that substance P receptor, neurokinin 1 was ... division of normal rats. Unilateral or bilateral injection ...
(Date:7/11/2014)... DUBLIN , July 11, 2014 ... the "International Biometrics Technology Market - Industry Analysis ... report to their offering. ... attacks and crimes has created a need for high ... methods used for personnel authentication such as username and ...
Breaking Biology News(10 mins):Virtual finger enables scientists to navigate and analyze complex 3D images 2International Biometrics Technology Market - Industry Analysis Size Share Growth Trends and Forecast to 2019 2
... the Hebrew University of Jerusalem have achieved, for the ... cells of Fragile X patients. The discovery paves the ... gene expression in Fragile X patients. Fragile X ... retardation, affecting hundreds of thousands of patients worldwide. The ...
... differences between plants and animals, subtle ones lie concealed within ... animal cells, the nucleus contains DNA, which condenses into ... counted at that stage, revealing the chromosome number ... number spans a relatively short range across animal species (2-296: ...
... (Santa Barbara, Calif.) After an announcement by federal ... searching for a way to treat Alzheimer,s Disease are ... them to test a therapy for a genetically predestined ... incredible," said UC Santa Barbara neuroscientist Ken Kosik, who ...
Cached Biology News:Stem cell research paves way for progress on dealing with Fragile X retardation 2Does polyploidy play a role in the onset of the Italian endemic flora? 2Clinical trials for Alzheimer's disease preventative drug to begin early 2013 2Clinical trials for Alzheimer's disease preventative drug to begin early 2013 3
Syd-2 (cL-19)...
Anti-MD-2 (IN) Host: rabbit polyclonal Species Reactivity: human, mouse, rat Applications: WB, IHC Storage: 4C...
TNFalpha-IP 2 (G-17)...
The product contains 2 ml Dynabeads® Epoxy (4.5 μm). Supplied at 4 x 10e8 beads/ml...
Biology Products: